FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002488336

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488336
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences354864
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTTAAATACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA21680.6109382749447676No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA14220.40071689435952934No Hit
CCTACCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA13760.3877541818837639No Hit
CCCTTTACACAATGTGGTTACCCAGCATTAATGCCTTTGTATGCATGTAT13480.379863835159385No Hit
GTGTAAAGGGAGCGGCAAAGCCCAAAAGCCCCACAATGCGTTGACATACT13150.3705644979485099No Hit
GTCTGTAAATACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA11940.33646692817530094No Hit
CCTCTCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA11740.330830966229316No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG10860.3060327336669823No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC9990.28151629920194776No Hit
ATTTACATACTCTGTGGAAGGCGGGTATTTTATATAAGAGAGAAACTACT9380.2643266152666937No Hit
ATGTAAGACCTTGGGCAAGACCTGGCGGGCGTTCACGGTGGTCTCCATGC8940.25192749898552685No Hit
GGACTCGTGCAGGTCTTGCATGGTCCCGTGCTGGTAGTTGATGTTCCTGG8440.2378375941205645No Hit
CTCACCAACCTCTTGTCCTCCAATTTGTCCTGGTTATCGCTGGATGTGTC8320.23445601695297352No Hit
GTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATATGTTCAGG8110.22853825690968937No Hit
AGCGAGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATACTCAAGAA8100.2282564588123901No Hit
GGATATTATCAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAACG7890.22233869876910592No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA7790.21952071779611343No Hit
GCGGTAGGAGTGCGAATCCACACTCCAAAAGACACCAAATACTCAAGAAC7730.21782992921231797No Hit
CCTAAACATCAGACAACTATTGTGGTTTCACATTTCCTGTCTTACTTTTG7610.21444835204472698No Hit
ATCCTAACCTTACCAAATATTTGCCCTTAGATAAAGGCATTAAACCTTAT7600.21416655394742773No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAGACC7460.2102213805852383No Hit
GGTTAGGATAGAACCTAGCAGGCATAATCAATTTTAATCTCCTTTTTTCG7440.20965778439063978No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGGCCG7060.1989494566932684No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTGATAATATCCTCTTTC6710.18908652328779477No Hit
GTATCGAGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG6620.18655034041210156No Hit
TCTCAATGTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGGC6590.18570494612020377No Hit
ACATCGAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGACC6490.18288696514721134No Hit
TTTACATACTCTGTGGAAGGCGGGTATTTTATATAAGAGAGAAACTACTC6280.17696920510392714No Hit
GCTTACTACTAATTCCCTGGATGATGGGTCTTCCAAATTACTTCCCACCC6170.17386942603363542No Hit
GTATACATGCATACAAAGGCATTAATGCTGGGTAACCACATTGTGTAAAG6120.1724604355471392No Hit
CTCCATATTCGGTGCAGGGTCCCCAGTCCTCGAGAAGATTGACGATATGG6010.16936065647684748No Hit
CCCAGTACAAAGATCATTAACCGAGTCTCCTCCCCCAACTCCTCCCAGTC6000.16907885837954822No Hit
ATATGTTCCTGTGGCAAGGTACCCCAACTTCCAATTACATATCCCATGAA5960.16795166599035122No Hit
CTCCTCTCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCG5880.16569728121195726No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC5800.1634428964335633No Hit
GGTTAGTGAGTGATTGGAGGTTGGGGACTGCAAATTTTGGCCAGGACACG5750.16203390594706704No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN5740.1617521078497678No Hit
CCTTTACCCCGTTGCTCGGCAACGGTCAGGTCTTTGCCAAGTGTTTGCGG5700.1606249154605708No Hit
CATTTAAACCCTAATAAAACCAAACGTTGGGGCTATTCCCTTAACTTCAT5680.1600613192659723No Hit
GCACTAAGGCAAGCTATTCTGTGTTGGGGTGAGTTAATGAATCTGGCCAC5650.15921592497407458No Hit
GCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT5650.15921592497407458No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG5570.1569615401956806No Hit
CTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGATTATGCCTGCTAGG5540.15611614590378284No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAAGATGATGGGATGGGAATACAAG5510.1552707516118851No Hit
GTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC5470.15414355922268813No Hit
GATCATTAACCGAGTCTCCTCCCCCAACTCCTCCCAGTCTTTAAATACAC5390.15188917444429417No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA5340.15048018395779791No Hit
GCCTAATCATCTCATGTTCATGTCCCACTGTTCAAGCCTCCAAGCTGTGC5230.1473804048875062No Hit
GACCTTTAACCGAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAATACAC5190.14625321249830922No Hit
AATATGGAGAGCACCACATCAGGATTCCTAGGACCCCTGCTCGTGTTACA5170.14568961630371072No Hit
CATCGAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGACCT5140.14484422201181296No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT5140.14484422201181296No Hit
GGGTAAAGGTGCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA5100.14371702962261598No Hit
AAGCGGTAGGAGTGCGAATCCACACTCCAAAAGACACCAAATACTCAAGA5000.14089904864962352No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG4970.1400536543577258No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGATTGCCGTG4970.1400536543577258No Hit
GAAGTTTCCGAAAACATTGTTTGATTTTGTGTACAATATGTTCCTGTGGC4910.13836286577393028No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG4880.13751747148203255No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGACCTGGCGGGCGTTC4850.1366720771901348No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGA4780.1346994905090401No Hit
GTAGTAAGCTATGTCAACGTTAATATGGGCCTAAAAATCAGACAACTATT4730.13329050002254386No Hit
ATTTATAGGAAGTTTCCGAAAACATTGTTTGATTTTGTGTACAATATGTT4710.13272690382794536No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGCGGTAAAAA4700.1324451057306461No Hit
GCACGATTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTGTTGCTGTACA4690.13216330763334685No Hit
CCCAGTACAAAGACCTTTAACCGAATCTCCTCCCCCAACTCCTCCCAGTC4650.13103611524414988No Hit
ATTGACAACAGTACCAGCAGCGCCTCCTCCTGCCTCCACCAATCGGCAGT4590.1293453266603544No Hit
GTAGTCAGCTATGTCAACGTTAATATGGGCCTAAACATCAGACAACTATT4550.1282181342711574No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA4520.12737273997925966No Hit
ATATAAGAGAGAAACTACTCGCAGCGCTTCATTTTGTGGGTCACCATATT4520.12737273997925966No Hit
CTCCTACCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCG4500.12680914378466115No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAGATC4430.12483655710356642No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTAAGGCA4300.12117318183867622No Hit
CTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCTCTTTTCCTCAT4290.12089138374137699No Hit
GTGTAAACAATATCTGCACCTTTACCCCGTTGCTCGGCAACGGTCAGGTC4280.12060958564407774No Hit
GGATAATAAGGTTTAATGCCTTTATCTAAGGGCAAATATTTGGTAAGGTT4280.12060958564407774No Hit
ATACAGCACTAAGGCAAGCTATTCTGTGTTGGGGTGAGTTAATGAATCTG4280.12060958564407774No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAATTGC4270.12032778754677849No Hit
GGATAGAACCTAGCAGGCATAATCAATTTTAATCTCCTTTTTTCGTTAAC4260.12004598944947924No Hit
GGCCTATACTTTCCTGCTGGTGGCTCCAGTTCCGGAACAGTAAACCCTGT4260.12004598944947924No Hit
CCTATAAATAGACCTATTGATTGGAAAGTATGTCAACGCATTGTGGGGCT4240.11948239325488075No Hit
GGGTTTACTGTTCCGGAACTGGAGCCACCAGCAGGAAAGTATAGGCCCCT4170.11750980657378601No Hit
CCCATAGGAATCTTGCGAAAGCCCAAGATGATGGGATGGGAATACAAGTG4170.11750980657378601No Hit
GCATTAATGCCTTTGTATGCATGTATACAAGCTAAGCAGGCTTTCACTTT4120.11610081608728977No Hit
CTCCTACCTGGTGTGCCTCTGGCCATTGATCCTTGTTGGGGTTGAAGTCC4110.11581901798999052No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA4060.1144100275034943No Hit
GGATTAAAGACAGGTACAGTAGAAGAATAAAGCCCAGTAAAGTTTCCCAC4010.11300103701699807No Hit
CAATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGG3990.11243744082239956No Hit
CTCCAGCACCTTCCACCAAGCTCTGCTAGATCCCAGAGTGAGGGGCCTAT3980.11215564272510031No Hit
TCACCATACAGCACTAAGGCAAGCTATTCTGTGTTGGGGTGAGTTAATGA3980.11215564272510031No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT3970.11187384462780109No Hit
GGCTTTCTTCCTGACTGCCGATTGGTGGAGGCAGGAGGAGGCGCTGCTGG3960.11159204653050184No Hit
GTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCCGCCTGTAACACG3960.11159204653050184No Hit
ACTCTTATCGGAACCGACAACTCTGTTGTCCTCTCTCGGAAATACACCTC3960.11159204653050184No Hit
TACATACTCTGTGGAAGGCGGGTATTTTATATAAGAGAGAAACTACTCGC3960.11159204653050184No Hit
GTATTGGGGGCCAAGTCTGTACAACATCTTGAATCCCTTTTTACCGCTAT3940.11102845033590333No Hit
CCCCAAAAGATTGCCGTGTGGTGGAGTGAACCCTGGCCCGAAGGATCCCA3930.11074665223860408No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACA3910.11018305604400559No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCTGATGGCC3900.10990125794670634No Hit
CCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAAGAGTCCTCTTATGT3890.10961945984940709No Hit
ATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGG3860.10877406555750936No Hit
GTATAGGCCCCTCACTCTGGGATCTAGCAGAGCTTGGTGGAAGGTGCTGG3850.10849226746021011No Hit
GATAATATCCTCTTTCAAATGAATATGAGGAAAAGAGGGAGTTTGCCAGT3840.10821046936291086No Hit
ATAATATCCTCTTTCAAATGAATATGAGGAAAAGAGGGAGTTTGCCAGTC3820.10764687316831237No Hit
GGTCTTGCATGGTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGAC3810.10736507507101312No Hit
GTGTTGGGGTGAGTTAATGAATCTGGCCACCTGGGTGGGAAGTAATTTGG3800.10708327697371386No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT3770.10623788268181614No Hit
GAATACAAGTGCAATTTCCGTCCGAAGGTTTTGTACAGCAACAAGAGGGA3740.1053924883899184No Hit
GTAGAAGAATAAAGCCCAGTAAAGTTTCCCACCTTATGAGTCCAAGGGAT3720.10482889219531989No Hit
ACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTT3720.10482889219531989No Hit
GGTCTATTTATAGGAAGTTTCCGAAAACATTGTTTGATTTTGTGTACAAT3680.10370169980612291No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAATCCCTTTTTACC3680.10370169980612291No Hit
GAATATGGAGAGCACCACATCAGGATTCCTAGGACCCCTGCTCGTGTTAC3650.10285630551422516No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTG3610.10172911312502818No Hit
CTTGTATTCCCATCCCATCATCTTGGGCTTTCGCAAGATTCCTATGGGAG3570.10060192073583119No Hit
GGAACATATTGTACACAAAATCAAACAATGTTTTCGGAAACTTCCTATAA3570.10060192073583119No Hit
CTTCTGGACTACCAAGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGAAC3550.10003832454123271No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACCTTGCCACAG3550.10003832454123271No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)