FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002488337

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488337
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences354864
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTTAAATACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA16980.4784931692141215No Hit
GTGTAAAGGGAGCGGCAAAGCCCAAAAGCCCCACAATGCGTTGACATACT13050.3677465169755174No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA12300.34661165967807384No Hit
CCCTTTACACAATGTGGTTACCCAGCATTAATGCCTTTGTATGCATGTAT12170.34294828441318365No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG12050.3395667072455927No Hit
CCTACCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA11700.32970377384011906No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGGCCG11090.31251408990486496No Hit
CCTCTCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA10420.2936336173858154No Hit
GTCTGTAAATACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA9930.2798255106181523No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC9400.2648902114612922No Hit
GTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATATGTTCAGG9280.26150863429370125No Hit
ATTTACATACTCTGTGGAAGGCGGGTATTTTATATAAGAGAGAAACTACT8510.23981018080165922No Hit
CTCACCAACCTCTTGTCCTCCAATTTGTCCTGGTTATCGCTGGATGTGTC8140.22938365120158707No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAGACC7740.21811172730961723No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA7560.21303936155823078No Hit
CCTAAACATCAGACAACTATTGTGGTTTCACATTTCCTGTCTTACTTTTG7560.21303936155823078No Hit
GGTTAGGATAGAACCTAGCAGGCATAATCAATTTTAATCTCCTTTTTTCG7400.20853059200144278No Hit
GGATATTATCAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAACG7300.20571261102845032No Hit
GGACTCGTGCAGGTCTTGCATGGTCCCGTGCTGGTAGTTGATGTTCCTGG7220.20345822625005636No Hit
ATGTAAGACCTTGGGCAAGACCTGGCGGGCGTTCACGGTGGTCTCCATGC6920.19500428333107894No Hit
ATCCTAACCTTACCAAATATTTGCCCTTAGATAAAGGCATTAAACCTTAT6760.190495513774291No Hit
CTCCATATTCGGTGCAGGGTCCCCAGTCCTCGAGAAGATTGACGATATGG6730.18965011948239324No Hit
GTATCGAGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG6730.18965011948239324No Hit
GCGGTAGGAGTGCGAATCCACACTCCAAAAGACACCAAATACTCAAGAAC6700.18880472519049551No Hit
GCTTACTACTAATTCCCTGGATGATGGGTCTTCCAAATTACTTCCCACCC6630.18683213850940078No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTGATAATATCCTCTTTC6440.1814779746607151No Hit
GTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC6360.1792235898823211No Hit
TCTCAATGTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGGC6170.17386942603363542No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGCCCGTGTG5880.16569728121195726No Hit
AGCGAGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATACTCAAGAA5880.16569728121195726No Hit
ACATCGAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGACC5770.16259750214166555No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN5740.1617521078497678No Hit
CATTTAAACCCTAATAAAACCAAACGTTGGGGCTATTCCCTTAACTTCAT5660.15949772307137383No Hit
CTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGATTATGCCTGCTAGG5640.15893412687677533No Hit
CCTTTACCCCGTTGCTCGGCAACGGTCAGGTCTTTGCCAAGTGTTTGCGG5630.15865232877947608No Hit
GCACTAAGGCAAGCTATTCTGTGTTGGGGTGAGTTAATGAATCTGGCCAC5590.1575251363902791No Hit
ATATGTTCCTGTGGCAAGGTACCCCAACTTCCAATTACATATCCCATGAA5550.1563979440010821No Hit
GTATACATGCATACAAAGGCATTAATGCTGGGTAACCACATTGTGTAAAG5450.15357996302808963No Hit
GGTTAGTGAGTGATTGGAGGTTGGGGACTGCAAATTTTGGCCAGGACACG5430.15301636683349115No Hit
TTTACATACTCTGTGGAAGGCGGGTATTTTATATAAGAGAGAAACTACTC5420.1527345687361919No Hit
CTCCTCTCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCG5400.1521709725415934No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAAGATGATGGGATGGGAATACAAG5370.15132557824969567No Hit
GCCTAATCATCTCATGTTCATGTCCCACTGTTCAAGCCTCCAAGCTGTGC5160.14540781820641147No Hit
GCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT5030.14174444294152125No Hit
GCACGATTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTGTTGCTGTACA5010.14118084674692277No Hit
GTAGTCAGCTATGTCAACGTTAATATGGGCCTAAACATCAGACAACTATT4980.14033545245502504No Hit
GGGTAAAGGTGCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA4970.1400536543577258No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG4970.1400536543577258No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC4810.13554488480093782No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA4810.13554488480093782No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA4810.13554488480093782No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA4800.13526308670363857No Hit
ATTTATAGGAAGTTTCCGAAAACATTGTTTGATTTTGTGTACAATATGTT4740.1335722981198431No Hit
GGATAGAACCTAGCAGGCATAATCAATTTTAATCTCCTTTTTTCGTTAAC4710.13272690382794536No Hit
ATATAAGAGAGAAACTACTCGCAGCGCTTCATTTTGTGGGTCACCATATT4710.13272690382794536No Hit
GTAGTAAGCTATGTCAACGTTAATATGGGCCTAAAAATCAGACAACTATT4700.1324451057306461No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG4700.1324451057306461No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG4660.1313179133414491No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGATTGCCGTG4630.13047251904955137No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAGATC4620.13019072095225212No Hit
GTGTAAACAATATCTGCACCTTTACCCCGTTGCTCGGCAACGGTCAGGTC4620.13019072095225212No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGA4610.1299089228549529No Hit
GAAGTTTCCGAAAACATTGTTTGATTTTGTGTACAATATGTTCCTGTGGC4600.12962712475765364No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT4580.12906352856305514No Hit
GTATTGGGGGCCAAGTCTGTACAACATCTTGAATCCCTTTTTACCGCTAT4450.12540015329816492No Hit
CTCCTACCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCG4420.1245547590062672No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTAAGGCA4410.12427296090896794No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT4410.12427296090896794No Hit
AATATGGAGAGCACCACATCAGGATTCCTAGGACCCCTGCTCGTGTTACA4410.12427296090896794No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCTGATGGCC4380.12342756661707022No Hit
GCATTAATGCCTTTGTATGCATGTATACAAGCTAAGCAGGCTTTCACTTT4360.12286397042247171No Hit
GTTTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCGGGGCCG4350.12258217232517246No Hit
GGCCTATACTTTCCTGCTGGTGGCTCCAGTTCCGGAACAGTAAACCCTGT4340.12230037422787321No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGCGGTAAAAA4320.12173677803327472No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT4300.12117318183867622No Hit
GGATTAAAGACAGGTACAGTAGAAGAATAAAGCCCAGTAAAGTTTCCCAC4250.11976419135217999No Hit
GGGTTTACTGTTCCGGAACTGGAGCCACCAGCAGGAAAGTATAGGCCCCT4210.11863699896298299No Hit
GTGTTGGGGTGAGTTAATGAATCTGGCCACCTGGGTGGGAAGTAATTTGG4200.11835520086568374No Hit
CTCCAGCACCTTCCACCAAGCTCTGCTAGATCCCAGAGTGAGGGGCCTAT4120.11610081608728977No Hit
ATTGACAACAGTACCAGCAGCGCCTCCTCCTGCCTCCACCAATCGGCAGT4100.11553721989269129No Hit
CTCCTACCTGGTGTGCCTCTGGCCATTGATCCTTGTTGGGGTTGAAGTCC4090.11525542179539204No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAATTGC4080.11497362369809279No Hit
CTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCTCTTTTCCTCAT4000.11271923891969882No Hit
GGCTTTCTTCCTGACTGCCGATTGGTGGAGGCAGGAGGAGGCGCTGCTGG4000.11271923891969882No Hit
ATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGG3980.11215564272510031No Hit
ATAATATCCTCTTTCAAATGAATATGAGGAAAAGAGGGAGTTTGCCAGTC3960.11159204653050184No Hit
GTTTGGGACTCTACCGTCCCCTTCTTCGTCTGCCGTTCCGGCCGACCACG3950.11131024843320259No Hit
GTTCAGGATAATAAGGTTTAATGCCTTTATCTAAGGGCAAATATTTGGTA3940.11102845033590333No Hit
AAGCGGTAGGAGTGCGAATCCACACTCCAAAAGACACCAAATACTCAAGA3920.11046485414130484No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAATCCCTTTTTACC3920.11046485414130484No Hit
ATACAGCACTAAGGCAAGCTATTCTGTGTTGGGGTGAGTTAATGAATCTG3920.11046485414130484No Hit
GATCATTAACCGAGTCTCCTCCCCCAACTCCTCCCAGTCTTTAAATACAC3880.10933766175210784No Hit
GTAGAAGAATAAAGCCCAGTAAAGTTTCCCACCTTATGAGTCCAAGGGAT3800.10708327697371386No Hit
CCTATAAATAGACCTATTGATTGGAAAGTATGTCAACGCATTGTGGGGCT3760.10595608458451689No Hit
GCTTTCTTCCTGACTGCCGATTGGTGGAGGCAGGAGGAGGCGCTGCTGGT3750.10567428648721763No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACCTTGCCACAG3750.10567428648721763No Hit
GTATAGGCCCCTCACTCTGGGATCTAGCAGAGCTTGGTGGAAGGTGCTGG3740.1053924883899184No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGACCTGGCGGGCGTTC3740.1053924883899184No Hit
GATAATATCCTCTTTCAAATGAATATGAGGAAAAGAGGGAGTTTGCCAGT3740.1053924883899184No Hit
ATATGGGAGAGGCAATAGTCGGAACAGGGTTTACTGTTCCGGAACTGGAG3730.10511069029261914No Hit
CTCCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCG3720.10482889219531989No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTG3710.10454709409802064No Hit
CTACCGTCCCCTTCTTCGTCTGCCGTTCCGGCCGACCACGGGGCGCACCT3700.10426529600072139No Hit
CATCGAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGACCT3690.10398349790342216No Hit
CTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGAAGAGAAACTGTTCT3680.10370169980612291No Hit
TCACCATACAGCACTAAGGCAAGCTATTCTGTGTTGGGGTGAGTTAATGA3650.10285630551422516No Hit
CCCCAAAAGATTGCCGTGTGGTGGAGTGAACCCTGGCCCGAAGGATCCCA3650.10285630551422516No Hit
GGTTGGGGCTTGGCCATAGGCCATCAGCGCATGCGTGGAACCTTTGTGGC3640.10257450741692593No Hit
CAATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGG3640.10257450741692593No Hit
GTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCCGCCTGTAACACG3620.10201091122232743No Hit
GGATAATAAGGTTTAATGCCTTTATCTAAGGGCAAATATTTGGTAAGGTT3570.10060192073583119No Hit
GGAACATATTGTACACAAAATCAAACAATGTTTTCGGAAACTTCCTATAA3560.10032012263853193No Hit
GAATACAAGTGCAATTTCCGTCCGAAGGTTTTGTACAGCAACAAGAGGGA3550.10003832454123271No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)