FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002488338

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488338
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences345600
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC30420.8802083333333334No Hit
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA28830.8342013888888888No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACGTAGCGCC24930.7213541666666666No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG20850.6032986111111112No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC19030.5506365740740741No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA13010.37644675925925924No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACCTTCCACCAAACTCTGCAAG12770.3695023148148148No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT11230.32494212962962965No Hit
ATACAAACCTTGCCAGCAAATCCGCCTCCTGCATCTACCAATCGCCAGTC9990.2890625No Hit
ATATAAGAGAGAAACAACACGTAGCGCCTCATTTTGTGGGTCACCATATT9300.2690972222222222No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCAGTACG8780.25405092592592593No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA8590.24855324074074073No Hit
GTCCCGCGCTGGTGGTTGAAGATCCTGGAATTAGAGGACAGACGGGCAAC8050.23292824074074076No Hit
TTATATAAGAGAGAAACAACACGTAGCGCCTCATTTTGTGGGTCACCATA8000.23148148148148145No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATCACAGAGAGTCCAA7940.22974537037037038No Hit
GCCTGAGGATGAGTGTTTCTCAAAGGTGGAGACAGCGGGGTAGGCTGCCT7910.22887731481481483No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACT7810.2259837962962963No Hit
CCCTTATCTAATGGCAAATATTTGGTAACATTTGGATAAAACCTAGCAGG7620.2204861111111111No Hit
GGTTTGTATTATGCCCTGAGCCTGAGGACTCCACCCCAAAAGGCCTCCGT7570.21903935185185186No Hit
ATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTCACGGTGGTCTCCATGC7490.216724537037037No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGAAAGGACAACAGAGT7340.21238425925925924No Hit
ACATAGAGGTTCCTTGAGCAGTAGTCGTGCAGGTTCTGCATGGTCCCGCG7300.21122685185185186No Hit
CATTTAAACCCTGACAAAACTAAAAGATGGGGTTACTCTTTAAATTTCAT7150.2068865740740741No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA6940.2008101851851852No Hit
CTCTATGACCGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA6730.1947337962962963No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT6730.1947337962962963No Hit
GTGTAAATGTATATTAGGAAAAGAGGGTGTTTTCCAATGAGGGTTAAAGA6690.1935763888888889No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTC6670.19299768518518517No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT6550.18952546296296297No Hit
GTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACC6500.1880787037037037No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG6450.18663194444444445No Hit
AGATAAGGGTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACT6430.18605324074074073No Hit
GCCTTAGAGTCTCCTGAGCATTGTTCACCTCACCATACTGCACTCAGGCA6080.17592592592592593No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA6070.1756365740740741No Hit
CTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTT6030.17447916666666666No Hit
CACTAATATGGGCCTAAAGTTTAGGCAACTTTTGTGGTTTCACATTTCTT5970.17274305555555555No Hit
ATATTAGTGTTGACATAGCTAACTACTAGGTCCCTAGATGCTGGATCTTC5950.17216435185185186No Hit
GTCATAGAGTATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCAGC5940.171875No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCCTTGTATGCCTGTATT5830.16869212962962965No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGAAGAGGA5820.1684027777777778No Hit
AGCTGGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATACTCTATGA5800.16782407407407407No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAGTCCCTTTTTACC5770.16695601851851852No Hit
GTATGGATCGGCAGAGGAGCCAGAAAGGTTCCACGCATGCGCTGATGGCC5760.16666666666666669No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC5680.16435185185185186No Hit
GCGCGGGACCATGCAGAACCTGCACGACTACTGCTCAAGGAACCTCTATG5560.16087962962962962No Hit
GTTTAATACCCTTATCTAATGGCAAATATTTGGTAACATTTGGATAAAAC5520.15972222222222224No Hit
CCTCTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGC5500.15914351851851852No Hit
CTACCGTACCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTCCTAAT5490.15885416666666666No Hit
ATCAAGGTATGTTGCCCGTCTGTCCTCTAATTCCAGGATCTTCAACCACC5440.1574074074074074No Hit
ATACTGCACTCAGGCAAGCAATTCTTTGCTGGGGGGAACTAATGACTCTA5380.15567129629629628No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT5380.15567129629629628No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC5360.1550925925925926No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC5330.15422453703703703No Hit
GGGTTTACTGTTCCTGAACTGGAGCCACCAGCAGGGAAATACAGGCCTCT5320.15393518518518517No Hit
TCCTTGGACTCATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACCG5310.15364583333333331No Hit
TCCTAGTACAAAGACCTTTAATCTAATCTCCTCCCCCAACTCCTCCCAGT5250.15190972222222224No Hit
GCCTAATCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC5210.15075231481481483No Hit
TCACCATACTGCACTCAGGCAAGCAATTCTTTGCTGGGGGGAACTAATGA5170.1495949074074074No Hit
ATGTGGTTATCCTGCTTTAATGCCCTTGTATGCCTGTATTCAATCTCGGC5120.14814814814814814No Hit
TAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACG5100.14756944444444445No Hit
CTTCTAGATACCGCCTCAGCTCTCTATCGGGAAGCCTTAGAGTCTCCTGA5050.14612268518518517No Hit
GTCCAAGGAATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGAC4910.14207175925925924No Hit
GAATACAGGTGCAATTTCCGTCCGAAGGTTTGGTACAGCAACAGGAGGGA4910.14207175925925924No Hit
GTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT4850.14033564814814817No Hit
CCTGTATTCCCATCCCATCATCCTGGGCTTTCGGAAAATTCCTATGGGAG4850.14033564814814817No Hit
GATACATAGAGGTTCCTTGAGCAGTAGTCGTGCAGGTTCTGCATGGTCCC4830.13975694444444442No Hit
CCTTATGAGTCCAAGGAATACTAACATTGAGATTCCCGAGATTGAGATCT4800.1388888888888889No Hit
GTATTATGCCCTGAGCCTGAGGACTCCACCCCAAAAGGCCTCCGTGCAGT4790.13859953703703703No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA4770.13802083333333334No Hit
TCTAGGGACCTAGTAGTTAGCTATGTCAACACTAATATGGGCCTAAAGTT4710.13628472222222224No Hit
CTTCTGGACTATCAAGGTATGTTGCCCGTCTGTCCTCTAATTCCAGGATC4700.13599537037037038No Hit
ATATGGGAGAGGCAGTAGTCGGAACAGGGTTTACTGTTCCTGAACTGGAG4660.13483796296296297No Hit
GACCTGGCCGTTGCCGGGCAACGGGGTAAAGGTTCAGATATTGTTTACAC4660.13483796296296297No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAG4610.13339120370370372No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT4590.1328125No Hit
GTATTTCCCTGCTGGTGGCTCCAGTTCAGGAACAGTAAACCCTGTTCCGA4590.1328125No Hit
CCCTGGGATCTTGCAGAGTTTGGTGGAAGGTTGTGGAGTTCCACTGCATG4580.13252314814814814No Hit
CCTCAAGGTCGGTCGTTGACATCACAGAGAGTCCAAGAGTCCTCTTATGT4540.13136574074074076No Hit
GTGCAGTGGGGTGAATCCCAGCCCGAATGCTCCAGCTCCTACCTTGTTGG4540.13136574074074076No Hit
GTGTAAACAATATCTGAACCTTTACCCCGTTGCCCGGCAACGGCCAGGTC4530.1310763888888889No Hit
CAGTAAACCCTGTTCCGACTACTGCCTCTCCCATATCGTCAATCTTCTCG4530.1310763888888889No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAG4510.13049768518518517No Hit
CTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACGTAGCGCCTC4420.12789351851851852No Hit
TTATTTACACACTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAA4270.12355324074074074No Hit
GAATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGA4220.12210648148148148No Hit
GGATAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAAT4210.12181712962962962No Hit
GGCATACAAGGGCATTAAAGCAGGATAACCACATTGTGTAAAAGGGGCAG4200.12152777777777778No Hit
CTATAAGCTGGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATACTC4190.12123842592592592No Hit
GTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC4190.12123842592592592No Hit
GTGCACACGGTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAA4180.12094907407407408No Hit
TCTAGATACCGCCTCAGCTCTCTATCGGGAAGCCTTAGAGTCTCCTGAGC4150.12008101851851852No Hit
ATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGA4080.11805555555555555No Hit
GTCTTGGTGTAAATGTATATTAGGAAAAGAGGGTGTTTTCCAATGAGGGT4070.11776620370370369No Hit
CTACTAGGTCCCTAGATGCTGGATCTTCCAAATTAACACCCACCCAGGTA4060.11747685185185186No Hit
CCTTTACCCCGTTGCCCGGCAACGGCCAGGTCTGTGCCAAGTGTTTGCTG4040.11689814814814815No Hit
TCCTTGAGCAGTAGTCGTGCAGGTTCTGCATGGTCCCGCGCTGGTGGTTG4040.11689814814814815No Hit
GTCTTTAACCCTCATTGGAAAACACCCTCTTTTCCTAATATACATTTACA4030.1166087962962963No Hit
ATCCCAGGGTGAGAGGCCTGTATTTCCCTGCTGGTGGCTCCAGTTCAGGA3990.1154513888888889No Hit
CCATATTCTTGGGAACAAGAGCTACAGCATGGGACAGAATCTTTCCACCA3980.11516203703703703No Hit
CACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACTCACAGT3970.11487268518518519No Hit
GAGCTACAGCATGGGACAGAATCTTTCCACCAGCAATCCTCTGGGATTCT3930.11371527777777778No Hit
ATATTATATAAGAGAGAAACAACACGTAGCGCCTCATTTTGTGGGTCACC3890.11255787037037038No Hit
ATGTATATTTGCGGGAAAGGACAACAGAGTTATCCGTCCCGAGAATGTTT3870.11197916666666667No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACA3860.11168981481481481No Hit
ACCTAGTAGTTAGCTATGTCAACACTAATATGGGCCTAAAGTTTAGGCAA3850.11140046296296297No Hit
GTCTCCACCTTTGAGAAACACTCATCCTCAGGCCATGCAGTGGAACTCCA3810.11024305555555555No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTG3810.11024305555555555No Hit
GACGGGGAGTCCGCGTAAAGAGAGGTGCGCCCCGTGGTCGGTCGAAACGG3810.11024305555555555No Hit
GTTAACAGGAAGTTTTCTAAAACATCCTTTGATTTTCTGTATGATGTGAT3780.10937500000000001No Hit
CTCCGAAAGACACCAAATACTCTATGACCGTTTCTCTTCCAAAAGTGAGA3760.1087962962962963No Hit
GGATAATAAGGTTTAATACCCTTATCTAATGGCAAATATTTGGTAACATT3740.10821759259259259No Hit
GTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATG3720.1076388888888889No Hit
GTTATGGGTCATTGCCACAAGATCACATCATACAGAAAATCAAAGGATGT3720.1076388888888889No Hit
GTTGTGGAGTTCCACTGCATGGCCTGAGGATGAGTGTTTCTCAAAGGTGG3700.10706018518518519No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGA3680.10648148148148148No Hit
AGATAGGGGCATTTGGTGGTCTATAAGCTGGAGGAGTGCGAATCCACACT3660.10590277777777779No Hit
CTCCCATATCGTCAATCTTCTCGAGGATTGGGGACCCTGCGCTGAACATG3610.10445601851851853No Hit
GCCCAGGATGATGGGATGGGAATACAGGTGCAATTTCCGTCCGAAGGTTT3580.10358796296296297No Hit
GGTTCTGCATGGTCCCGCGCTGGTGGTTGAAGATCCTGGAATTAGAGGAC3580.10358796296296297No Hit
ATCCCAGCCCGAATGCTCCAGCTCCTACCTTGTTGGCGTCTGGCCAGGTG3580.10358796296296297No Hit
GAATACAGGCATACAAGGGCATTAAAGCAGGATAACCACATTGTGTAAAA3530.1021412037037037No Hit
TCCCAGCCCGAATGCTCCAGCTCCTACCTTGTTGGCGTCTGGCCAGGTGT3510.1015625No Hit
CCTCAGGCTCAGGGCATAATACAAACCTTGCCAGCAAATCCGCCTCCTGC3500.10127314814814814No Hit
GAACATGGAGAACATCACATCAGGATTCCTAGGACCCCTGCTCGTGTTAC3500.10127314814814814No Hit
CGGTAGTTCCCCCTAGAAAATTGAGAGAAGTCCACCACGAGTCTAGACTC3500.10127314814814814No Hit
ATGTCATTGGATGTTATGGGTCATTGCCACAAGATCACATCATACAGAAA3490.10098379629629631No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)