FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002488339

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488339
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences345600
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA26120.755787037037037No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACGTAGCGCC23890.6912615740740741No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC23560.6817129629629629No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG21110.6108217592592592No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC16350.47309027777777773No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA14050.40653935185185186No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACCTTCCACCAAACTCTGCAAG11700.3385416666666667No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT11030.31915509259259256No Hit
ATACAAACCTTGCCAGCAAATCCGCCTCCTGCATCTACCAATCGCCAGTC9550.2763310185185185No Hit
ATATAAGAGAGAAACAACACGTAGCGCCTCATTTTGTGGGTCACCATATT8890.2572337962962963No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCAGTACG8860.25636574074074076No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA8100.234375No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACT7730.22366898148148145No Hit
GTCCCGCGCTGGTGGTTGAAGATCCTGGAATTAGAGGACAGACGGGCAAC7590.21961805555555552No Hit
CTCTATGACCGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA7490.216724537037037No Hit
GCCTGAGGATGAGTGTTTCTCAAAGGTGGAGACAGCGGGGTAGGCTGCCT7370.21325231481481483No Hit
GGTTTGTATTATGCCCTGAGCCTGAGGACTCCACCCCAAAAGGCCTCCGT7330.2120949074074074No Hit
TTATATAAGAGAGAAACAACACGTAGCGCCTCATTTTGTGGGTCACCATA6980.20196759259259262No Hit
CATTTAAACCCTGACAAAACTAAAAGATGGGGTTACTCTTTAAATTTCAT6660.19270833333333334No Hit
GTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACC6650.19241898148148148No Hit
CCCTTATCTAATGGCAAATATTTGGTAACATTTGGATAAAACCTAGCAGG6540.1892361111111111No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT6540.1892361111111111No Hit
AGATAAGGGTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACT6280.18171296296296297No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGAAAGGACAACAGAGT6260.18113425925925927No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT6240.18055555555555555No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCCTTGTATGCCTGTATT6210.1796875No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC6150.1779513888888889No Hit
GTGTAAATGTATATTAGGAAAAGAGGGTGTTTTCCAATGAGGGTTAAAGA6120.17708333333333331No Hit
GTCATAGAGTATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCAGC6060.1753472222222222No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA5950.17216435185185186No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATCACAGAGAGTCCAA5910.17100694444444445No Hit
GCCTTAGAGTCTCCTGAGCATTGTTCACCTCACCATACTGCACTCAGGCA5870.16984953703703703No Hit
ACATAGAGGTTCCTTGAGCAGTAGTCGTGCAGGTTCTGCATGGTCCCGCG5750.16637731481481483No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAGTCCCTTTTTACC5650.16348379629629628No Hit
GCCTAATCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC5620.16261574074074073No Hit
CTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTT5560.16087962962962962No Hit
GCGCGGGACCATGCAGAACCTGCACGACTACTGCTCAAGGAACCTCTATG5550.16059027777777776No Hit
ATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTCACGGTGGTCTCCATGC5520.15972222222222224No Hit
CACTAATATGGGCCTAAAGTTTAGGCAACTTTTGTGGTTTCACATTTCTT5480.1585648148148148No Hit
GTTTAATACCCTTATCTAATGGCAAATATTTGGTAACATTTGGATAAAAC5470.15827546296296297No Hit
ATATTAGTGTTGACATAGCTAACTACTAGGTCCCTAGATGCTGGATCTTC5460.1579861111111111No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTC5420.15682870370370372No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG5400.15625No Hit
GTATGGATCGGCAGAGGAGCCAGAAAGGTTCCACGCATGCGCTGATGGCC5280.1527777777777778No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC5260.15219907407407407No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA5250.15190972222222224No Hit
CTTCTAGATACCGCCTCAGCTCTCTATCGGGAAGCCTTAGAGTCTCCTGA5230.15133101851851852No Hit
CTACCGTACCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTCCTAAT5200.15046296296296297No Hit
GTCCAAGGAATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGAC5180.14988425925925927No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGAAGAGGA5150.1490162037037037No Hit
ATCAAGGTATGTTGCCCGTCTGTCCTCTAATTCCAGGATCTTCAACCACC5150.1490162037037037No Hit
ATGTGGTTATCCTGCTTTAATGCCCTTGTATGCCTGTATTCAATCTCGGC5120.14814814814814814No Hit
ATACTGCACTCAGGCAAGCAATTCTTTGCTGGGGGGAACTAATGACTCTA5110.1478587962962963No Hit
CAGTAAACCCTGTTCCGACTACTGCCTCTCCCATATCGTCAATCTTCTCG5110.1478587962962963No Hit
GTATTATGCCCTGAGCCTGAGGACTCCACCCCAAAAGGCCTCCGTGCAGT5100.14756944444444445No Hit
CCTCTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGC5090.14728009259259262No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA4970.14380787037037038No Hit
GTGTAAACAATATCTGAACCTTTACCCCGTTGCCCGGCAACGGCCAGGTC4890.14149305555555555No Hit
TCCTTGGACTCATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACCG4790.13859953703703703No Hit
TCACCATACTGCACTCAGGCAAGCAATTCTTTGCTGGGGGGAACTAATGA4790.13859953703703703No Hit
GTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT4780.1383101851851852No Hit
GGGTTTACTGTTCCTGAACTGGAGCCACCAGCAGGGAAATACAGGCCTCT4750.13744212962962962No Hit
AGCTGGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATACTCTATGA4680.13541666666666669No Hit
CTCCCATATCGTCAATCTTCTCGAGGATTGGGGACCCTGCGCTGAACATG4660.13483796296296297No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT4650.1345486111111111No Hit
GTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATG4580.13252314814814814No Hit
GACCTGGCCGTTGCCGGGCAACGGGGTAAAGGTTCAGATATTGTTTACAC4570.13223379629629628No Hit
CTTCTGGACTATCAAGGTATGTTGCCCGTCTGTCCTCTAATTCCAGGATC4510.13049768518518517No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA4500.13020833333333331No Hit
GTATTTCCCTGCTGGTGGCTCCAGTTCAGGAACAGTAAACCCTGTTCCGA4500.13020833333333331No Hit
GATACATAGAGGTTCCTTGAGCAGTAGTCGTGCAGGTTCTGCATGGTCCC4460.12905092592592593No Hit
CTCCGAAAGACACCAAATACTCTATGACCGTTTCTCTTCCAAAAGTGAGA4430.12818287037037038No Hit
TCTAGGGACCTAGTAGTTAGCTATGTCAACACTAATATGGGCCTAAAGTT4360.1261574074074074No Hit
GTCTTTAACCCTCATTGGAAAACACCCTCTTTTCCTAATATACATTTACA4340.1255787037037037No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT4320.125No Hit
ATATGGGAGAGGCAGTAGTCGGAACAGGGTTTACTGTTCCTGAACTGGAG4290.12413194444444445No Hit
CTGCCTAATCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGT4280.1238425925925926No Hit
CCCTGGGATCTTGCAGAGTTTGGTGGAAGGTTGTGGAGTTCCACTGCATG4280.1238425925925926No Hit
CCTTTACCCCGTTGCCCGGCAACGGCCAGGTCTGTGCCAAGTGTTTGCTG4280.1238425925925926No Hit
CTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACGTAGCGCCTC4270.12355324074074074No Hit
TAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACG4260.12326388888888888No Hit
CCTGTATTCCCATCCCATCATCCTGGGCTTTCGGAAAATTCCTATGGGAG4260.12326388888888888No Hit
CCTTATGAGTCCAAGGAATACTAACATTGAGATTCCCGAGATTGAGATCT4250.12297453703703703No Hit
TCTAGATACCGCCTCAGCTCTCTATCGGGAAGCCTTAGAGTCTCCTGAGC4200.12152777777777778No Hit
GTGCAGTGGGGTGAATCCCAGCCCGAATGCTCCAGCTCCTACCTTGTTGG4180.12094907407407408No Hit
ATCCCAGGGTGAGAGGCCTGTATTTCCCTGCTGGTGGCTCCAGTTCAGGA4080.11805555555555555No Hit
GTCTTGGTGTAAATGTATATTAGGAAAAGAGGGTGTTTTCCAATGAGGGT4080.11805555555555555No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAG4040.11689814814814815No Hit
GTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC4020.11631944444444443No Hit
CACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACTCACAGT3980.11516203703703703No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGA3970.11487268518518519No Hit
GTTATGGGTCATTGCCACAAGATCACATCATACAGAAAATCAAAGGATGT3920.11342592592592593No Hit
CCCCTATCTTATCAACACTTCCGGAGACTACTGTTGTTAGACGACGAGGC3870.11197916666666667No Hit
TTATTTACACACTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAA3830.11082175925925924No Hit
GTTGTGGAGTTCCACTGCATGGCCTGAGGATGAGTGTTTCTCAAAGGTGG3800.1099537037037037No Hit
GAATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGA3790.10966435185185185No Hit
ATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGA3770.10908564814814815No Hit
GGCATACAAGGGCATTAAAGCAGGATAACCACATTGTGTAAAAGGGGCAG3760.1087962962962963No Hit
GAATACAGGTGCAATTTCCGTCCGAAGGTTTGGTACAGCAACAGGAGGGA3750.10850694444444445No Hit
CCATATTCTTGGGAACAAGAGCTACAGCATGGGACAGAATCTTTCCACCA3730.10792824074074076No Hit
CCCTTATAAAGAATTTGGAGCTACTGTGGAGTTACTCTCGTTTTTGCCTT3690.10677083333333333No Hit
GGATAATAAGGTTTAATACCCTTATCTAATGGCAAATATTTGGTAACATT3660.10590277777777779No Hit
TCTCGGGGCCGCTTGGGACTCTCTCGTCCCCTTCTCCGTCTGCCGTTTCG3650.10561342592592593No Hit
CTACTAGGTCCCTAGATGCTGGATCTTCCAAATTAACACCCACCCAGGTA3640.10532407407407407No Hit
GACATAGCCCATGAAATTTAAAGAGTAACCCCATCTTTTAGTTTTGTCAG3630.10503472222222222No Hit
GTATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCAGCTTATAGAC3630.10503472222222222No Hit
GTCCCCAATCCTCGAGAAGATTGACGATATGGGAGAGGCAGTAGTCGGAA3620.10474537037037036No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC3610.10445601851851853No Hit
GTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGG3600.10416666666666667No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACA3580.10358796296296297No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTG3560.10300925925925926No Hit
ATATTATATAAGAGAGAAACAACACGTAGCGCCTCATTTTGTGGGTCACC3540.10243055555555557No Hit
GCTTTGGGGCATGGACATTGACCCTTATAAAGAATTTGGAGCTACTGTGG3520.10185185185185185No Hit
GTATTGGGGGCCAAGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGT3510.1015625No Hit
AATATACATCGTTTCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTG3510.1015625No Hit
ATGTATATTTGCGGGAAAGGACAACAGAGTTATCCGTCCCGAGAATGTTT3480.10069444444444445No Hit
CTATAAGCTGGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATACTC3470.1004050925925926No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)