FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488340

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488340
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences489955
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTACACCTTTACCCCGTTGCGCGGCAACGGTCAGGTCTCTGCCAAGTG18250.37248318723148044No Hit
CTATATAAGAGAGAAACTACACGCAGCGCTTCATTTTGTGGGTCACCATA14260.29104713698196777No Hit
ATTAATAGGTGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAAAG13960.2849241256850119No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA13050.2663509914175792No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG12340.25185986468145033No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC12140.24777785715014647No Hit
GTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGC11940.24369584961884252No Hit
CATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA11880.2424712473594514No Hit
ATATAAGAGAGAAACTACACGCAGCGCTTCATTTTGTGGGTCACCATATT11790.24063434397036462No Hit
CCTATTAATAATGTCCTCCTGTAAATGAATGTTAGGAAAAGAGGGAGTTT11670.23818513945158226No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG11650.2377769386984519No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT11640.2375728383218867No Hit
GGTGTAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTG11620.23716463756875633No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAG11370.23206212815462643No Hit
CCTTTACCCCGTTGCGCGGCAACGGTCAGGTCTCTGCCAAGTGTTTGCTG11360.23185802777806125No Hit
TTCCCCCACTGTGTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC11250.22961292363584412No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG11120.22695961874049658No Hit
GCATGGGAGGTTGGTCTTCCAAACCTCGACAAGGCATGGGGACGAATCTT10720.21879560367788878No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC10710.2185915033013236No Hit
GCACGATTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTGTTGCTGTACA10430.21287669275749815No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT9710.1981814656448041No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG9610.19614046187915218No Hit
ACCAAGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGAACATCAACTACC9590.1957322611260218No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG9300.18981335020563112No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACA9300.18981335020563112No Hit
GGACTCGTGCAGGTCTTGCATGGTCCCGTGCTGGTAGTTGATGTTCCTGG9180.1873641456868488No Hit
TCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG9170.1871600453102836No Hit
TCCCATGGCTGCTCGGGTGTGCTGCCAACTGGATCCTGCGCGGGACGTCC9020.18409853966180567No Hit
ATTGACAACAGTGCCCGCAGCGCCTCCTCCTGCCTCCACCAATCGGCAGT9000.18369033890867528No Hit
GCATTCGACAAGGCATGGGGACGAATCTTTCTGTTCCCAATCCTCTGGGA8680.17715912685858906No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG7990.1630762008755906No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTGAAATTAATTATGCCTGCTAGG7980.16287210049902542No Hit
CTTTTGGAGTGTGGATTCGCACCCCTCCCGCTTACAGACCACCAAATGCC7980.16287210049902542No Hit
TCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG7960.16246389974589504No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA7770.15858599259115633No Hit
ATACCACATCATCCATATAACTGAAAGCCACACAGTGGGGGAAAGCCCTA7700.15715728995519998No Hit
TACCTTGGTAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG7660.1563408884489392No Hit
CCCACGTGTCCTGGCCAAAATTCGCAGTCCCCAACCTCCAATCACTCACC7550.15409578430672205No Hit
TACCCTGGTAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG7530.15368758355359166No Hit
GCCTCAGACCTTTAGCCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAA7450.1520547805410701No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT7350.15001377677541816No Hit
GTATACACGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA7300.14899327489259218No Hit
CTGTAAGAGGGCCCACATATTGTTGACACCTATTAATAATGTCCTCCTGT7260.1481768733863314No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGC7250.1479727730097662No Hit
CTACTGTACCTGTCTTTAACCCTGAGTGGCAAACTCCCTCTTTTCCTAAC7200.14695227112694023No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT7120.14531946811441868No Hit
TTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACAC6870.1402169587002888No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTCAAATGTATA6820.13919645681746284No Hit
GTCCGGCAGATGTGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTGC6790.13858415568776725No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC6790.13858415568776725No Hit
GGGTAAAGGTGTAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA6680.13633905154555012No Hit
GCCATGGGAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGTTGTCAGTC6650.1357267504158545No Hit
ATATTTTCCTGCTGGTGGCTCCAGTTCCGGAACAGTAAACCCTGTTCCGA6600.13470624853302854No Hit
CTTTATATGCGTGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACT6590.13450214815646336No Hit
CTTTTACACAATGTGGCTATCCTGCCTTGATGCCTTTATATGCGTGTATA6560.13388984702676776No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT6500.1326652447673766No Hit
GTCTTTAAACAAACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA6460.13184884326111582No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTTTCCATGC6430.13123654213142022No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTCAGGCA6410.13082834137828986No Hit
GGTGCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTG6390.13042014062515944No Hit
GCCTCCTAGTACAAAGACCTTTAGCCTAATCTCCTCCCCCAACTCCTCCC6390.13042014062515944No Hit
ATATAACTGAAAGCCACACAGTGGGGGAAAGCCCTACGAACCACTGAACA6310.1287873376126379No Hit
TCCTCAGGCCATGCAGTGGAACTCCACCACATTCCACCAAGCTCTGCTAG6270.1279709361063771No Hit
GTGTAAACAATATCTACACCTTTACCCCGTTGCGCGGCAACGGTCAGGTC6240.12735863497668154No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG6230.12715453460011633No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTC6130.1251135308344644No Hit
GTCGAGGTTTGGAAGACCAACCTCCCATGCTGTAGCTCTTGTTCCCAAGA6030.12307252706881244No Hit
GCAGACGAAGAAGGGGACGGTAGAGTCCCAAACGGCCCCGAGACGGGTCG5960.12164382443285608No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCTTAT5890.12021512179689971No Hit
CGTGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC5880.12001102142033453No Hit
TATCTACACCTTTACCCCGTTGCGCGGCAACGGTCAGGTCTCTGCCAAGT5880.12001102142033453No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCCGATGGCC5800.11837821840781297No Hit
GAGACGGGTCGTCCGCGGGATTCAGCGCCGACGGGACGTAGACAAAGGAC5760.11756181690155218No Hit
TCCTTTGTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCG5640.11511261238276985No Hit
TTATTAATAGGTGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA5630.11490851200620464No Hit
CCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAGTG5490.11205110673429193No Hit
ATATAGGCCCCTCACTCTGGGATCTAGCAGAGCTTGGTGGAATGTGGTGG5470.11164290598116154No Hit
CCATACAGCACTCAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATC5470.11164290598116154No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCACA5450.11123470522803114No Hit
GTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC5410.11041830372177036No Hit
CCATATAACTGAAAGCCACACAGTGGGGGAAAGCCCTACGAACCACTGAA5400.11021420334520517No Hit
CCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAAGAGTCCTCTTATGT5390.11001010296863999No Hit
GGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAG5370.10960190221550958No Hit
CTTGTATTCCCATCCCATCATCCTGGGCTTTCGCAAGATTCCTATGGGAG5370.10960190221550958No Hit
GTCGAATGCTGTAGCTCTTGTTCCCAAGAATATGGTGACCCACAAAATGA5340.108989601085814No Hit
GATTTAGATCTTCTGCGACGCGGCGATTGAGACCTTCGTCTGCGAGGCGA5320.1085814003326836No Hit
CTCCAGACCTGCTGCGAGCAAAACAAGCTGCTAGGAGTTCCGCAGTATGG5320.1085814003326836No Hit
GACAAAGGACGTCCCGCGCAGGATCCAGTTGGCAGCACACCCGAGCAGCC5280.10776499882642285No Hit
GTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAAA5240.10694859732016207No Hit
CCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAAAGGGACTCAAGATG5220.10654039656703167No Hit
CAGTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTG5200.10613219581390128No Hit
CAATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGG5130.1047034931779449No Hit
GGTCTGGAGCGAACCTTATCGGGACTGACAACTCTGTTGTCCTCTCTCGG5110.10429529242481453No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC5060.10327479054198854No Hit
GGTTAGGATAAAACCTAGCAGGCATAATTAATTTCAATCTCCTTTTTTCA5040.10286658978885817No Hit
GCCTAATCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC5010.10225428865916258No Hit
CCCCTATCTTATCAACACTTCCGGAAACTACTGTTGTTAGACGACGAGGC5010.10225428865916258No Hit
GCCTGAGGATGACTGTCTCTTAGAGGTGGAGAGATGGGAGTAGGCTGTCT4960.1012337867763366No Hit
GGAACATATTGTACAAAAACTCAAGCAATGTTTTCGGAAACTGCCTGTAA4910.10021328489351063No Hit
GAATACAAGTGCAGTTTCCGTCCGAAGGTTTTGTACAGCAACAAGAGGGA4910.10021328489351063No Hit
CCTTTAGCCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAACAG4900.10000918451694545No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)