FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488341

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488341
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences489955
Sequences flagged as poor quality0
Sequence length35-151
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTACACCTTTACCCCGTTGCGCGGCAACGGTCAGGTCTCTGCCAAGTG19570.3994244369380862No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG13290.27124940045514384No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG12720.25961567899092775No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA12420.2534926676939719No Hit
CTATATAAGAGAGAAACTACACGCAGCGCTTCATTTTGTGGGTCACCATA12330.2516557643048852No Hit
ATTAATAGGTGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAAAG12020.2453286526313641No Hit
CCTTTACCCCGTTGCGCGGCAACGGTCAGGTCTCTGCCAAGTGTTTGCTG11950.24389994999540776No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT11460.23389903154371322No Hit
CATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA11190.22838832137645296No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG10960.22369401271545344No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT10750.2194079048075844No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG10720.21879560367788878No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC10710.2185915033013236No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG10630.21695870028880204No Hit
ATATAAGAGAGAAACTACACGCAGCGCTTCATTTTGTGGGTCACCATATT10580.21593819840597606No Hit
CCTATTAATAATGTCCTCCTGTAAATGAATGTTAGGAAAAGAGGGAGTTT10550.21532589727628046No Hit
TTCCCCCACTGTGTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC10550.21532589727628046No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC10440.21308079313406333No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAG10100.20614138033084672No Hit
TCCCATGGCTGCTCGGGTGTGCTGCCAACTGGATCCTGCGCGGGACGTCC9960.20328397505893397No Hit
GTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGC9650.19695686338541293No Hit
GCATGGGAGGTTGGTCTTCCAAACCTCGACAAGGCATGGGGACGAATCTT9600.19593636150258698No Hit
GCACGATTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTGTTGCTGTACA9450.19287485585410905No Hit
GGTGTAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTG9380.19144615321815267No Hit
TCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG8870.18103703401332777No Hit
GGACTCGTGCAGGTCTTGCATGGTCCCGTGCTGGTAGTTGATGTTCCTGG8600.1755263238460675No Hit
ACCAAGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGAACATCAACTACC8550.17450582196324152No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTGAAATTAATTATGCCTGCTAGG8430.1720566174444592No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG8260.16858691104285087No Hit
CTTTTGGAGTGTGGATTCGCACCCCTCCCGCTTACAGACCACCAAATGCC8140.16613770652406853No Hit
ATTGACAACAGTGCCCGCAGCGCCTCCTCCTGCCTCCACCAATCGGCAGT8110.16552540539437297No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACA8110.16552540539437297No Hit
GTATACACGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA7950.16225979936932983No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA7480.15266708167076568No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG7240.147768672633201No Hit
GCATTCGACAAGGCATGGGGACGAATCTTTCTGTTCCCAATCCTCTGGGA7200.14695227112694023No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC7120.14531946811441868No Hit
CTGTAAGAGGGCCCACATATTGTTGACACCTATTAATAATGTCCTCCTGT7050.1438907654784623No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT6810.13899235644089764No Hit
TCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG6760.13797185455807165No Hit
CTACTGTACCTGTCTTTAACCCTGAGTGGCAAACTCCCTCTTTTCCTAAC6710.1369513526752457No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT6660.1359308507924197No Hit
GAGACGGGTCGTCCGCGGGATTCAGCGCCGACGGGACGTAGACAAAGGAC6650.1357267504158545No Hit
CTTTATATGCGTGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACT6560.13388984702676776No Hit
GGGTAAAGGTGTAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA6500.1326652447673766No Hit
GTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC6490.13246114439081141No Hit
ATACCACATCATCCATATAACTGAAAGCCACACAGTGGGGGAAAGCCCTA6250.12756273535324672No Hit
GCCATGGGAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGTTGTCAGTC6240.12735863497668154No Hit
TACCTTGGTAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG6230.12715453460011633No Hit
CTTTTACACAATGTGGCTATCCTGCCTTGATGCCTTTATATGCGTGTATA6220.12695043422355115No Hit
GTCCGGCAGATGTGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTGC6150.12552173158759478No Hit
ATATTTTCCTGCTGGTGGCTCCAGTTCCGGAACAGTAAACCCTGTTCCGA6070.12388892857507323No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCCGATGGCC6040.12327662744537762No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTCAAATGTATA6030.12307252706881244No Hit
CTTCATCTGCCGTTCCGGCCGACCACGGGGCGCACCTCTCTTTACGCGGT5920.1208274229265953No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCTTAT5870.11980692104376933No Hit
GTGTAAACAATATCTACACCTTTACCCCGTTGCGCGGCAACGGTCAGGTC5770.11776591727811739No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGC5750.11735771652498699No Hit
TACCCTGGTAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG5730.11694951577185661No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT5680.11592901388903062No Hit
GGTGCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTG5640.11511261238276985No Hit
TATCTACACCTTTACCCCGTTGCGCGGCAACGGTCAGGTCTCTGCCAAGT5630.11490851200620464No Hit
GTCTTTAAACAAACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA5630.11490851200620464No Hit
TCCTCAGGCCATGCAGTGGAACTCCACCACATTCCACCAAGCTCTGCTAG5610.11450031125307426No Hit
ATATAACTGAAAGCCACACAGTGGGGGAAAGCCCTACGAACCACTGAACA5590.11409211049994386No Hit
GTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAAA5560.11347980937024829No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTC5540.1130716086171179No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTCAGGCA5530.11286750824055271No Hit
GGTTAGGATAAAACCTAGCAGGCATAATTAATTTCAATCTCCTTTTTTCA5520.1126634078639875No Hit
GTCGAGGTTTGGAAGACCAACCTCCCATGCTGTAGCTCTTGTTCCCAAGA5510.1124593074874223No Hit
CGGTGGTTTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG5490.11205110673429193No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCACA5450.11123470522803114No Hit
TTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACAC5420.11062240409833557No Hit
GTCGAATGCTGTAGCTCTTGTTCCCAAGAATATGGTGACCCACAAAATGA5400.11021420334520517No Hit
TCCTTTGTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCG5380.10980600259207479No Hit
GTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCGGGGCCG5340.108989601085814No Hit
GCAGACGAAGAAGGGGACGGTAGAGTCCCAAACGGCCCCGAGACGGGTCG5330.1087855007092488No Hit
ATATAGGCCCCTCACTCTGGGATCTAGCAGAGCTTGGTGGAATGTGGTGG5190.10592809543733608No Hit
TTATTAATAGGTGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA5190.10592809543733608No Hit
GCCTGAGGATGACTGTCTCTTAGAGGTGGAGAGATGGGAGTAGGCTGTCT5170.10551989468420568No Hit
CGTGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC5140.1049075935545101No Hit
CTTGTATTCCCATCCCATCATCCTGGGCTTTCGCAAGATTCCTATGGGAG5020.10245838903572776No Hit
CTCCAGACCTGCTGCGAGCAAAACAAGCTGCTAGGAGTTCCGCAGTATGG5010.10225428865916258No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCACATCTGCCGGACCGTGTG5000.10205018828259738No Hit
GGTCTGGAGCGAACCTTATCGGGACTGACAACTCTGTTGTCCTCTCTCGG4990.10184608790603218No Hit
GCCTAATCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC4930.10062148564664103No Hit
GGATGGGGCTTGGCTATCGGCCATCGGCGCATGCGTGGAACCTTTGTGGC4920.10041738527007582No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)