FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002488342

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488342
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences249665
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTACCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA13840.5543428193779665No Hit
CTTTAGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGA9360.37490236917469405No Hit
GGTTACTCTTCCAAACCTCGACAAGGCATGGGGACGAATCTTTCTGTTCC9080.3636873410369896No Hit
CTCCTACCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCG8850.3544749964953037No Hit
GTAACACGAGCAGGGGTCCTAGGAATCCTGATGTTGTGTTCTCCATGTTC8560.3428594316383955No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG8000.32042937536298643No Hit
GACCTTTAACCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAAC7950.31842669176696775No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGTCTAGTTT7420.29719824564916986No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG6980.2795746300042056No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC6710.26876013858570486No Hit
CTATATAAGAGAGAAACTACACGCAGCGCTTCATTTTGTGGGTCACCATA6680.26755852842809363No Hit
CCCCCAACTCCTCCCAGTCTTTAAACAAACAGTCTTTGAAGTATGCCTCA6300.2523381330983518No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT6070.24312578855666594No Hit
GTATGTAAATAATGTCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG6050.24232471511825845No Hit
ATTGACAACAGTGCCAGCAGCGCCTCCTCCTGCCTCCACCAATCGGCAGT5970.2391204213646286No Hit
GTTACTCTTCCAAACCTCGACAAGGCATGGGGACGAATCTTTCTGTTCCC5680.22750485650772034No Hit
GGGTTGAGTTGATGAATCTGGCCACCTGGGTGGGAAGTAATTTGGAAGAC5630.22550217291170166No Hit
GGAATCGTGCAGGTCTTGCATGGTCCCGTGCTGGTAGTTGATGTTCCTGG5580.22349948931568303No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG5520.22109626900046064No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCCTCCTGT5410.21669036508921957No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTACTGAGAGTCCAA5210.20867963070514492No Hit
CTACTGTACCTGTCTTTAATCCTGAATGGCAAACTCCCTCCTTTCCTAAC5180.2074780205475337No Hit
GATTTAGATCTTCTGCGACGCGGCGATTGAGACCTTCGTCTGCGAGGCGA5130.20547533695151501No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG5080.20347265335549639No Hit
GTCTATTCGAGATCTCCTCGACACCGCCTCTGCTCTGTATCGGGAGGCCT5080.20347265335549639No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGTGTTCACGGTGGTCTCCATGC5060.2026715799170889No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTGAAATTAATTATGCCTGCTAGG4990.19986782288266275No Hit
ATATAAGAGAGAAACTACACGCAGCGCTTCATTTTGTGGGTCACCATATT4960.19866621272505158No Hit
AGACCAACCTCCCATGCTGTAGCTCTTGTTCCCAAGAATATGGTGACCCA4950.19826567600584785No Hit
GCTTTAGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGG4930.19746460256744036No Hit
GATCATTAACCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAAC4880.19546191897142168No Hit
CATTTAAACCCTAATAAAACCAAACGTTGGGGTTACTCCCTTAACTTCAT4590.18384635411451344No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCTTAT4450.17823884004566118No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA4370.1750345462920313No Hit
TCCTCAGGCCATGCAGTGGAACTCCACCACATTCCACCAAGCTCTGCTAG4340.1738329361344201No Hit
ACTCACCCCAACACAGAATAGCTTGCCTGAGTGCTGTATGGTGAGGTGAA4260.17062864238079026No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACA4190.16782488534636414No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTCAGGCA4090.1638195181543268No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTG4070.16301844471591934No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGTCTAGT4040.16181683455830814No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA4010.16061522440069695No Hit
CTTTTGGAGTGTGGATTCGCACTCCTACCGCTTACAGACCACCAAATGCC3920.15701039392786334No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT3920.15701039392786334No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG3890.15580878377025215No Hit
GCACGACTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTGTTGCTGTACA3870.15500771033184466No Hit
GTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC3850.15420663689343722No Hit
GCTTTGGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGG3830.15340556345502973No Hit
CTTTTACACAATGTGGCTATCCTGCCTTGATGCCTTTATATGCATGTATA3810.15260449001662227No Hit
GGGTTCACCCCATCACACGGCGGTCTTTTGGGGTGGAGCCCTCAGGCTCA3800.15220395329741854No Hit
CATGTATACAAGCGAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC3790.1518034165782148No Hit
TGCCCACAGCCTCCTAGTACAAAGATCATTAACCTAATCTCCTCCCCCAA3790.1518034165782148No Hit
CTATTAATAATGTCCTCCTGTAAATGAATGTTAGGAAAGGAGGGAGTTTG3770.15100234313980734No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA3720.1489996595437887No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCCGATGGCC3660.1465964392285663No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTG3640.1457953657901588No Hit
ATATTTTCCTGCTGGTGGCTCCAGTTCCGGAACAGCAAACCCTGTTCCGA3620.14499429235175135No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACGAGAAAAACCCC3500.14018785172130654No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA3490.1397873150021028No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA3450.1381851681252879No Hit
GCCATGGGAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGTTGTCAGTC3440.13778463140608416No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA3430.13738409468688043No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC3380.13538141109086177No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG3380.13538141109086177No Hit
CGGTAGGAGTGCGAATCCACACTCCAAAAGACACCAAATACTCAAGAACA3340.13377926421404682No Hit
GCACACCCGAGCAGCCATGGGAAGGAGGTGTATTTCCGAGAGAGGACAAC3330.13337872749484309No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC3280.13137604389882443No Hit
GGTTAGGATAAAACCTAGCAGGCATAATTAATTTCAATCTCCTTTTTTCA3270.1309755071796207No Hit
CCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGACA3260.13057497046041697No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCC3220.12897282358360201No Hit
GTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTAT3160.12656960326837963No Hit
CTCCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCG3130.12536799311076843No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCCGGTCTCTGCCAAGTGTTTGCTG3120.12496745639156469No Hit
CGGATGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTCTACGTCC3110.12456691967236097No Hit
CTTTGGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGA3100.12416638295315724No Hit
CATACTGCGGAACTCCTAGCAGCTTGTTTTGCTCGCAGCCGGTCTGGAGC3080.12336530951474976No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTGC3080.12336530951474976No Hit
ATCTCAATCTCGGGAATCTCAATGTTAGTATCCCTTGGACTCATAAGGTG3060.12256423607634229No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAAA3020.12096208919952736No Hit
GGGTAAAGGTGCAGATATTGGTTACACAGAAAGGCCTTGTAAGTTGGCGA3010.12056155248032364No Hit
CCTTATGAGTCCAAGGGATACTAACATTGAGATTCCCGAGATTTAGATCT3000.1201610157611199No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGACCTGGTGGGTGTTC2970.11895940560350869No Hit
ACATTACTGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGACC2970.11895940560350869No Hit
ATATCTGCACCTTTACCCCGTTGCCCGGCAACGGTCCGGTCTCTGCCAAG2970.11895940560350869No Hit
CTTGTATTCCCATCCCATCATCCTGGGCTTTCGCAAGATTCCTATGGGAG2950.11815833216510124No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGCAGGGCTTTCCCCCACTGTT2940.1177577954458975No Hit
GTCCAAGGGATACTAACATTGAGATTCCCGAGATTTAGATCTTCTGCGAC2930.11735725872669377No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC2930.11735725872669377No Hit
GTGCTGGTGAACAGACCAATTTATGCCCACAGCCTCCTAGTACAAAGATC2910.11655618528828629No Hit
CTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGAAGAGAAACTGTTCT2870.11495403841147137No Hit
GTACAGTAGAAGAATAAAGCCCAGTAAAGTTTCCCACCTTATGAGTCCAA2860.11455350169226763No Hit
GAAGACCAACCTCCCATGCTGTAGCTCTTGTTCCCAAGAATATGGTGACC2840.11375242825386017No Hit
GTATACAAGCGAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCCTTT2840.11375242825386017No Hit
CTTGTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTA2810.11255081809624896No Hit
CATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA2790.11174974465784152No Hit
TCCTTTGTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCG2740.10974706106182285No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTGCGAACCACTGAACA2720.10894598762341537No Hit
GCCTGAGGATGACTGTCTCTTAGAGGTGGAGAGATGGGAGTAGGCTGTCT2710.10854545090421164No Hit
ATTTCAATCTCCTTTTTTCATTAACTGTAAGAGGGCCCACATATTGTTGA2710.10854545090421164No Hit
GTGATGGGGTGAACCCTGGCCCGAATGCTCCCGCTCCTACCTGATTTGCC2680.10734384074660044No Hit
GGATAGCCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAAT2670.10694330402739671No Hit
CAGTTTACTAGTGCCATTTGTTCAGTGGTTCGCAGGGCTTTCCCCCACTG2670.10694330402739671No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT2660.10654276730819297No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG2660.10654276730819297No Hit
GTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCGGGGCCG2620.10494062043137804No Hit
GGTCTTGCATGGTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGAC2610.10454008371217431No Hit
GAATACAAGTGCAGTTTCCGTCCGAAGGTTTTGTACAGCAACAAGAGGGA2600.1041395469929706No Hit
GTAGAGGCCCAAACGGCCCCGAGACGGGTCGTCCGCGGGATTCAGCGCCG2590.10373901027376685No Hit
GAATAAAGCCCAGTAAAGTTTCCCACCTTATGAGTCCAAGGGATACTAAC2560.10253740011615564No Hit
CTCTGGGATCTAGCAGAGCTTGGTGGAATGTGGTGGAGTTCCACTGCATG2550.10213686339695191No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC2540.10173632667774819No Hit
CCCCAAAAGACCGCCGTGTGATGGGGTGAACCCTGGCCCGAATGCTCCCG2510.10053471652013699No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)