FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002488344

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488344
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences412160
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACACCCGTG20090.48743206521739124No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCGGAAAGTCCAA20020.4857336956521739No Hit
CCTCCTGCATATAGACCCCCAAATGCCCCTATCTTATCAACACTTCCGGA19230.4665663819875776No Hit
ACTCACAACTGTGCCAGCAGCACCTCCTCCTGCCTCCACCAATCGGCAGT15540.37703804347826086No Hit
GTTGTGAGTATGCCCTGAGCCAGAGGGCTCCACCCCAACAAGCCCCCATG13750.33360830745341613No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACATCACCATACGG11020.26737189440993786No Hit
ATACCATATCATCCATATAACTGAAAGCCAGACAGTGGGGGAAAGCCCTA11000.2668866459627329No Hit
TCCTCAGGCCATGCAGTGGAATTCCACCACTTTCCATCAAACGCTTCAAG10640.25815217391304346No Hit
GTGTAAAAGGGGCAGCGAACCCCAAAAGACCCACAATTCGTTGACATACT10400.25232919254658387No Hit
GGATAATAGGGTTTGATTCCTTTATCTAAGGGCAAGTATTTGGTAACATT10080.24456521739130438No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAATACATAAACC10030.2433520962732919No Hit
CTATTATCCAGAGCATGTAGTTAATCATTACTTCCAGACGAGACATTATT9800.2377717391304348No Hit
GCTCAGAATACTGCCTCTGCCATATCGTCAACCTTCTCGAAGACTGGGGA9720.23583074534161494No Hit
GCCTAGCAGCCATGGAAACGATGTATACTTGCGGGAGAGCACGACAGAAT9500.23049301242236025No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGTGTAAAA9320.22612577639751552No Hit
ATATGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTATGCC9260.22467003105590064No Hit
ATATGTTCCTGTGGCAAGGAGCCCCAACTCCCAATTACATATCCCATGAA9150.2220011645962733No Hit
ATATAAAAGAGAGACGACACGTAGCGCCTCATTTTGCGGGTCACCATATT8920.21642080745341613No Hit
GCACCGGACCATGCAGAACCTGCACGACTCCTGCTCAAGGAACCTCTATG8480.20574534161490682No Hit
GTCCGGTGCTGGTGGTTGATGATCCTGGAATTAGAGGACAAACGGGCAAC8380.20331909937888198No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTTTTGTCAACGAGAAAAACCCC8360.20283385093167705No Hit
CATTTAAACCCTCACAAAACCAAACGATGGGGATACTCCCTTAATTTCAT8040.1950698757763975No Hit
GTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGC7940.19264363354037267No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAATACATAAATC7800.1892468944099379No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG7620.18487965838509318No Hit
TGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAAGAA7390.17929930124223603No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACATCACCATACGGCACTCAGGCA7390.17929930124223603No Hit
GTATTTGGTAACATTGGGATAAAATCTAGCAGGCATAATTAATTTCAATC7350.17832880434782608No Hit
GTATGGATCGGCAGCGGAGACATAAAGGTTCCGCGCATGCGCTGATGGCC7270.1763878105590062No Hit
GTCTTATATAAAAGAGAGACGACACGTAGCGCCTCATTTTGCGGGTCACC7040.17080745341614906No Hit
TATCCACATTGTGTAAAAGGGGCAGCGAACCCCAAAAGACCCACAATTCG6980.16935170807453417No Hit
CCAAAAGACACCAAATATTCAAGAACAGTGTCTCTTCCAAAAGTAAGACA6960.16886645962732919No Hit
TTCAAGAACAGTGTCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACA6960.16886645962732919No Hit
CCCTTAATTTCATGGGATATGTAATTGGGAGTTGGGGCTCCTTGCCACAG6890.1671680900621118No Hit
ATTCTGAGCAGGGCTCACTGTTCCTGAACTGGAGCCACCAGCAGGAAAGT6810.1652270962732919No Hit
CATGTATACAAGCTAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC6780.16449922360248448No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTGTCTCTTCCAAAAGTAAGA6770.16425659937888198No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATATGGTATTGGGGGC6760.16401397515527952No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC6490.15746312111801242No Hit
GGTGTAAGCAATTTGTGGGACCCCTAACAGTAAATGAAAACAGGAGATTG6480.15722049689440995No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCTGCATATAGACCCCCAAATGCC6470.15697787267080746No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC6370.1545516304347826No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCATCAACCACC6340.15382375776397517No Hit
GCTTTAGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGCAG6270.15212538819875776No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTAT6120.1484860248447205No Hit
ATATAAGACACCCGCTTTCCAAAGAGTATGTAAATAATGTCTCGTCTGGA6100.14800077639751552No Hit
CAGTAAGCCCTGCTCAGAATACTGCCTCTGCCATATCGTCAACCTTCTCG6070.1472729037267081No Hit
ATCTAAGGGCAAGTATTTGGTAACATTGGGATAAAATCTAGCAGGCATAA6050.1467876552795031No Hit
CTGTTAGGGGTCCCACAAATTGCTTACACCTATCAACAATGTCCTCCTGC6010.14581715838509315No Hit
CCTCTATGTTTCCCTCATGTTGCTGTACAAAACCTACGGACGGAAACTGC6000.1455745341614907No Hit
CCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAATTCCACCACTTT5940.14411878881987578No Hit
AATTAAGGGAGTATCCCCATCGTTTGGTTTTGTGAGGGTTTAAATGTATA5930.14387616459627328No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAG5920.14363354037267081No Hit
ATGTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCTAAAC5910.14339091614906832No Hit
ATGTATACTTGCGGGAGAGCACGACAGAATTGTCAGTCCCGATAAGTTTT5830.14144992236024845No Hit
CTTTTATATAAGACACCCGCTTTCCAAAGAGTATGTAAATAATGTCTCGT5800.140722049689441No Hit
ACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCTGCATGGTCCGGTG5750.13950892857142858No Hit
GGAACATATTGTACAAAAACTCAAATTATGTTTTAGGAAACTTCCTGTAA5750.13950892857142858No Hit
GGGCTCACTGTTCCTGAACTGGAGCCACCAGCAGGAAAGTACAGAGCCCT5750.13950892857142858No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGCAGAGGA5740.13926630434782608No Hit
GTACAGGGTCCCCAGTCTTCGAGAAGGTTGACGATATGGCAGAGGCAGTA5720.13878105590062112No Hit
GCTTACACCTATCAACAATGTCCTCCTGCAAATGAATGTCAGGAAAAGAA5720.13878105590062112No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAG5690.13805318322981366No Hit
GTTCTTGAATATTTGGTGTCTTTTGGAGTGTGGATTCGCACTCCTCCTGC5670.1375679347826087No Hit
ATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGG5640.13684006211180125No Hit
GTTTACAGGAAGTTTCCTAAAACATAATTTGAGTTTTTGTACAATATGTT5620.13635481366459629No Hit
GGTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGCATAA5570.13514169254658384No Hit
GTATAAAGAATTTGGAGCTTCTGCAGAATTACTCTCTTTTTTGCCTTCTG5570.13514169254658384No Hit
GGATTAAAGTTAGGTACAGTAGAAGAATAAAGCCCCGTAAAGTTTCCCAC5570.13514169254658384No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTC5540.1344138198757764No Hit
CTTTTACACAATGTGGATATCCTGCTTTAATGCCTTTATATGCATGTATA5500.13344332298136646No Hit
ACCTATGGGAGTGGGCCTCAGTCCGTTTCTCTTGGCTCAGTTTACTAGTG5480.1329580745341615No Hit
CCTTATGAGTCCAAGGAATACTAACATTGGGATTCCCGAGATTGAGATCT5430.13174495341614906No Hit
CAGTTGGACCCTGCATTCAAAGCCAACTCAGAAAATCCAGATTGGGACCT5410.1312597049689441No Hit
GTAGAAGAATAAAGCCCCGTAAAGTTTCCCACCTTATGAGTCCAAGGAAT5390.13077445652173914No Hit
GTGTTGGGGTGAGTTGATGAATCTAGCCACCTGGGTGGGAGGTAATTTGG5380.13053183229813664No Hit
CAATAGGCCTGTTTACAGGAAGTTTCCTAAAACATAATTTGAGTTTTTGT5360.13004658385093168No Hit
GACCAGGCCGTTGCCGAGCAACGGGGTAAAGGTTCAGATACTGTTTACTT5360.13004658385093168No Hit
GAATACAGGTGCAGTTTCCGTCCGTAGGTTTTGTACAGCAACATGAGGGA5360.13004658385093168No Hit
GTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTATGCCGCTGT5350.1298039596273292No Hit
GACGGGGAGTCCGCGTAAAGAGAGGTGCGCCCCGTGGTCGGTCGGTACGG5340.12956133540372672No Hit
CTTTAATCCTGATTGGCAAACTCCTTCTTTTCCTGACATTCATTTGCAGG5300.12859083850931677No Hit
GGATATCCACATTGTGTAAAAGGGGCAGCGAACCCCAAAAGACCCACAAT5280.1281055900621118No Hit
ACCAAATACTTGCCCTTAGATAAAGGAATCAAACCCTATTATCCAGAGCA5230.12689246894409936No Hit
GAATACTAACATTGGGATTCCCGAGATTGAGATCTTCTGCGACGCGGTGA5140.12470885093167701No Hit
GTACAGACTTGGCCCCCAATACCATATCATCCATATAACTGAAAGCCAGA5130.12446622670807454No Hit
GTCCAACTGATGATCGGGGAAGAATCCCAGTGGATTGGGGACAGAAAGAT5080.12325310559006211No Hit
CTTCTGGACTATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATC5040.12228260869565219No Hit
GTTGATAGGTGTAAGCAATTTGTGGGACCCCTAACAGTAAATGAAAACAG5020.1217973602484472No Hit
GTCCAAGGAATACTAACATTGGGATTCCCGAGATTGAGATCTTCTGCGAC5010.12155473602484472No Hit
TCCTTGGACTCATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACTG5010.12155473602484472No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA5000.12131211180124223No Hit
CAGTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTG4990.12106948757763976No Hit
GTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCTAAACAG4990.12106948757763976No Hit
GACATAAAGGTTCCGCGCATGCGCTGATGGCCTATGGCCAAGCCCCAACC4980.12082686335403726No Hit
TTCTTCTACTGTACCTAACTTTAATCCTGATTGGCAAACTCCTTCTTTTC4960.12034161490683229No Hit
GAATAAAGCCCCGTAAAGTTTCCCACCTTATGAGTCCAAGGAATACTAAC4950.12009899068322982No Hit
CCCTTAGATAAAGGAATCAAACCCTATTATCCAGAGCATGTAGTTAATCA4900.1188858695652174No Hit
CCCTTATCTCCACCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAA4860.11791537267080747No Hit
ATCCAGAGCATGTAGTTAATCATTACTTCCAGACGAGACATTATTTACAT4830.1171875No Hit
GTGCACACGGTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAA4800.11645962732919254No Hit
ATTTACATACTCTTTGGAAAGCGGGTGTCTTATATAAAAGAGAGACGACA4740.11500388198757763No Hit
ACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAA4740.11500388198757763No Hit
TCACCATACGGCACTCAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGA4720.11451863354037267No Hit
GCTTTATTCTTCTACTGTACCTAACTTTAATCCTGATTGGCAAACTCCTT4700.1140333850931677No Hit
GCTCTGTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACATCACCA4660.11306288819875776No Hit
CCTATCAACAATGTCCTCCTGCAAATGAATGTCAGGAAAAGAAGGAGTTT4650.11282026397515528No Hit
GGGTAAAGGTTCAGATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGA4600.11160714285714285No Hit
CCCAATTACATATCCCATGAAATTAAGGGAGTATCCCCATCGTTTGGTTT4580.11112189440993789No Hit
AGTCTGTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTT4550.11039402173913043No Hit
CCTCAAGGTCGGTCGTTGACATTGCGGAAAGTCCAAGAGTCCTCTTATGT4510.1094235248447205No Hit
ATGTAAGACCTTGGGCAGGTTCCAGTGGGGGTTCACGGTGGTTTCCATGC4410.10699728260869565No Hit
GTTCAGATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAA4410.10699728260869565No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGCATAAA4390.10651203416149069No Hit
ATATAACTGAAAGCCAGACAGTGGGGGAAAGCCCTACGAACCACTGAACA4380.10626940993788819No Hit
ATTCTGTGTTGGGGTGAGTTGATGAATCTAGCCACCTGGGTGGGAGGTAA4370.10602678571428571No Hit
AGATAAGGGAGTAGGCTGCCTTCCTGACTGCCGATTGGTGGAGGCAGGAG4360.10578416149068323No Hit
CAGTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATT4330.10505628881987578No Hit
CCATATTCTTGGGAACAAGAGCTACAGCATGGGAGGTTGGTCTTCCAAAC4320.10481366459627328No Hit
GTATGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACATGCTCTGG4310.10457104037267081No Hit
GTCGTTGACATTGCGGAAAGTCCAAGAGTCCTCTTATGTAAGACCTTGGG4260.10335791925465838No Hit
GAATACTGCCTCTGCCATATCGTCAACCTTCTCGAAGACTGGGGACCCTG4250.1031152950310559No Hit
ACATAAATCTTTAACCTAATCTCCTCCCCCAACTCCTCCCACTCAGTAAA4230.10263004658385091No Hit
CTCATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACTGTACCTAAC4220.10238742236024845No Hit
GGTCCTAGGAGTCCTGATGCGATGTTCTCCATGTTCGGTACAGGGTCCCC4200.10190217391304347No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAGGTTCCAGTGGGTGTTC4180.10141692546583853No Hit
CAGTGAGCCCTGCTCAGAATACTGCCTCTGCCATATCGTCAACCTTCTCG4170.10117430124223603No Hit
TCCTAGCCGCTTGTTTTGCTCGCAGCCGGTCTGGGGCAAAACTTATCGGG4160.10093167701863354No Hit
CCATATAACTGAAAGCCAGACAGTGGGGGAAAGCCCTACGAACCACTGAA4150.10068905279503106No Hit
GCATAAAGGGACTCAAGATGTTGTACAGACTTGGCCCCCAATACCATATC4150.10068905279503106No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)