FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488347

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488347
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences472033
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTAAAGGGAGCGGCAAAGCCCAGAAGCCCCACAATTCGTTGACACACT21750.46077286969343245No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA20840.4414945565246498No Hit
CCCTTTACACAATGTGGTTACCCAGCCTTAATGCCTTTGTATGCATGTAT17700.37497378361258643No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAGATC17650.3739145356362797No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG15430.3268839254882604No Hit
CATCTAAACCCTGCTAAAACTAAACGTTGGGGATACTCTCTTAACTTCAT15300.3241298807498628No Hit
GTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATGTGTTCAGG15120.32031658803515856No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAATGCTTCCGGA13970.2959538845801035No Hit
GTTTAGATGTATACCCAAAGACAAAAGAAAATTGGTAACAGTGGTAAAAA13780.29192874227013793No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC11840.25082992078943633No Hit
ATTTACATACTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACT11410.24172038819319838No Hit
GTGGTGGAGTGAACCCTGGCCCGAAGGATCCCACTCCTACCTGGTGTGCC11360.2406611402168916No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAATTGC11200.23727154669271003No Hit
GCAATAAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATCTGGCCAC10250.21714583514288194No Hit
CTCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACCTTACCTCAG9930.21036664809451883No Hit
GTAAACTATGTCAACGTTAATATGGGCCTAAAATTCAGACAACTATTGTG9720.2059178065940305No Hit
ATGTAAGACCTTGGGCAAGACCTGGCGGATGTTCACGGTGGTCTCCATGC9660.2046467090224624No Hit
CTCCATATTCGGTGCAGGGTCCCCAGTCCTCGAGAAGATTGACGATATGG8820.18685134302050915No Hit
GTATACATGCATACAAAGGCATTAAGGCTGGGTAACCACATTGTGTAAAG8650.18324989990106622No Hit
GCACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGCACCTCTATG8320.17625886325744175No Hit
CTCCCCCACACGGCAATCTTTTGGGGTGGAGCCCTCAGGCTCAGGGCATA8310.1760470136621804No Hit
GGATATTATCAATAGATGTCAACAATATGTAGGCCCTCTTACAGTTAACG8300.17583516406691904No Hit
ACATAGAGGTGCCTTGAGCAGGAATCGTGCAGGTCTTGCATGGTCCCGTG8110.17181002175695345No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTACACCTCACCATACAG8060.1707507737806467No Hit
TTTACATACTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACTC7970.16884412742329455No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC7720.16354788754176086No Hit
GCCTAATCATCTCATGTTCATGTCCCACTGTTCAAGCCTCCAAGCTGTGC7600.16100569239862467No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG7520.1593108956365339No Hit
GTAGTAGGCTATGTCAACGTTAATATGGGCCTAAAATTCAGACAACTATT7400.15676870049339772No Hit
GTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC7210.1527435581834321No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG7180.15210800939764804No Hit
CTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGATTATGCCTGCTAGG7000.14829471668294378No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG7000.14829471668294378No Hit
ATATGTTCCTGAGGTAAGGTACCCCAACTTCCAATTACATATCCCATGAA6920.14659991992085297No Hit
ATGTAGGCCCTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGATTATG6800.1440577247777168No Hit
GCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT6710.14215107842036467No Hit
CTCCAAAAGACACCAAATACTCAACAACAGTTTGTCTTCCAAAAGTAAGA6630.1404562816582739No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCCGCTTACAGACCACCAAATGCC6420.13600744015778557No Hit
GTTTCGCTCCAGACCGGCTGCGAGCAAAACAAGCTGCTAGGAGTTCCGCA6390.13537189137200153No Hit
ATTTAATCCTGACTGGCAAACTCCCTCTTTTCCTCACATTCATTTGAAAG6370.13494819218147883No Hit
CCTTTACCCCGTTGCTCGGCAACGGTCAGGTCTTTGCCAAGTGTTTGCTG6350.13452449299095615No Hit
ATTGACAACAGTACCAGCAGCGCCTCCTCCTGCCTCCACCAATCGGCAGT6330.13410079380043347No Hit
CTGTAAGAGGGCCTACATATTGTTGACATCTATTGATAATATCCTCTTTC6320.1338889442051721No Hit
GTATTGGGGGCCAAGTCTGTACAACATCTTGAATCCCTTTTTACCACTGT6180.1309230498715132No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCTGATGGCC6100.12922825310942243No Hit
CTCCCATAGGAATCTTACGAAAGCCCAAGATGATGGGATGGGAATACAAG6080.12880455391889972No Hit
ATCCTAACCGGACCAAATATTTGCCCTTAGATAAAGGCATTAAACCTTAT6080.12880455391889972No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA6060.12838085472837704No Hit
GGGTAAAGGTTCAGATATTGTTGACACAGAAAGGCCTTGTAAGTTGGCGA6060.12838085472837704No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG6040.12795715553785433No Hit
GTCCAAGGGATACTAACATTGAGGTTCCCGAGATTGAGATCTTCTGCGAC6020.12753345634733165No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT5990.12689790756154762No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGATTGCCGTG5980.12668605796628624No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATG5880.12456756201367276No Hit
GTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCGGGGCCG5860.12414386282315007No Hit
GCCTTAATGCCTTTGTATGCATGTATACAAGCTAAGCAGGCTTTCACTTT5850.12393201322788872No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA5760.1220253668705366No Hit
GGATTAAATACAGGTACAGTAGAAGAATAAAGCCCAGTAAAGTTTCCCAC5730.12138981808475255No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGA5670.12011872051318445No Hit
GCCTACTACTAATTCCCTGGATGCTGGGTCGTCCAAATTACTTCCCACCC5630.11927132213213906No Hit
CTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACTCGCAGCGCC5610.11884762294161637No Hit
AGTTAAGAGAGTATCCCCAACGTTTAGTTTTAGCAGGGTTTAGATGTATA5590.11842392375109369No Hit
ATATAAGAGAGAAACTACTCGCAGCGCCTCATTTTGTGGGTCACCATATT5590.11842392375109369No Hit
CTGCCTAATCATCTCATGTTCATGTCCCACTGTTCAAGCCTCCAAGCTGT5550.11757652537004827No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT5550.11757652537004827No Hit
ATACCTTCCTGCTGGTGGCTCCAGTTCCGGAACAGTAAACCCTGTTCCAA5540.11736467577478694No Hit
TCCTTTGTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCG5530.11715282617952559No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACA5510.1167291269890029No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAATCCCTTTTTACC5470.1158817286079575No Hit
GTACAAAGATCATTAATCTAGTCTCCTCCCCCAACTCCTCCCAGTCTTTA5400.11439878144112806No Hit
GGATAGAACCTAGCAGGCATAATCAATTTTAATCTCCTTTTTTCGTTAAC5390.1141869318458667No Hit
GGATACTAACATTGAGGTTCCCGAGATTGAGATCTTCTGCGACGCGGCGA5350.11333953346482131No Hit
CCTTATGAGTCCAAGGGATACTAACATTGAGGTTCCCGAGATTGAGATCT5330.11291583427429862No Hit
GGATAATAAGGTTTAATGCCTTTATCTAAGGGCAAATATTTGGTCCGGTT5280.11185658629799189No Hit
GGTTGGGGCTTGGCCATAGGCCATCAGCGCATGCGTGGAACCTTTGTGGC5260.1114328871074692No Hit
AATATGGAGAGCACCACATCAGGATTCCTAGGACCCCTGCTCGTATTACA5220.11058548872642379No Hit
CTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGAAGACAAACTGTTGT5170.10952624075011705No Hit
GGCCTACATATTGTTGACATCTATTGATAATATCCTCTTTCAAATGAATG5140.108890691964333No Hit
GTTAAGAGAGTATCCCCAACGTTTAGTTTTAGCAGGGTTTAGATGTATAC5030.10656034641645817No Hit
CTCCTACCTGGTGTGCCGCTGGCCATTGATCCTTGTTGGGATTGAAGTCC5030.10656034641645817No Hit
GTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACTCGCAGCGCCTCAT5010.10613664722593547No Hit
ATCTGAACCTTTACCCCGTTGCTCGGCAACGGTCAGGTCTTTGCCAAGTG4970.10528924884489008No Hit
CTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAGATCATT4940.10465370005910604No Hit
TTATTATCCTGAACACATAGTTAATCATTACTTCCAAACTAGGCATTATT4930.10444185046384467No Hit
ATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGG4880.10338260248753794No Hit
GTCTTTAAATACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA4860.10295890329701525No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTCTA4860.10295890329701525No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGACCTGGCGGATGTTC4840.10253520410649256No Hit
GTACAAAACCTTCGGACGGAAATTGCACTTGTATTCCCATCCCATCATCT4840.10253520410649256No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTGC4810.10189965532070851No Hit
GTTTTGCTCCAGACCGGCTGCGAGCAAAACAAGCTGCTAGGAGTTCCGCA4800.10168780572544717No Hit
GTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCCGCCTGTAATACG4800.10168780572544717No Hit
GAATATGGAGAGCACCACATCAGGATTCCTAGGACCCCTGCTCGTATTAC4790.10147595613018581No Hit
TCTAAACCCTGCTAAAACTAAACGTTGGGGATACTCTCTTAACTTCATGG4750.10062855774914042No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)