FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002488348

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488348
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences387328
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTATGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG17070.44071174818241904No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN16520.4265118968935889No Hit
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC16430.4241882848645076No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA15810.40818117977528096No Hit
CTCATTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA12560.32427296761401186No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACACCCGTG11870.3064586087243886No Hit
ATATAAAAGAGAGTCCACACGTAGCGCCTCATTTTGCGGGTCACCATATT11030.28477156311962987No Hit
ACTCACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT10590.27341168208856576No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG9810.2532737111698612No Hit
GTTGTGAGTATGCCCTGAGCCTGAGGGCTCCACCCCAACAGTCCCCCATG9460.24423744216787838No Hit
CCTCCTGCATATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA9280.23959021810971579No Hit
ATATGTTCCTGTGGCAATGTGCCCCAACACCCAATTACATATCCCATGAA8990.23210302379378717No Hit
CTTTTATATAAGATACCCGCCTTCCAAAGAGTGTGTAAATAATGTCTCGT8980.2318448446794448No Hit
TCCAAAGAGTGTGTAAATAATGTCTCGTCTGGAAGTAATGATTAACCACA8140.21015779907468607No Hit
GATGTATACTTGCGGGAGAGCACGACAGAATTGTCAGTCCCGATGAGTTT7690.19853973892927956No Hit
GTCCGGTGCTGGTTGTTGATGATCCTGGAATTAGAGGACAAACGGGCAAC7210.186147141440846No Hit
ATGCAGTGGAACTCCACCACTTTCCACCAAACTCTTCAAGATCCCAGAGT7210.186147141440846No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGG7050.18201627561136813No Hit
CTCTTTGGAAGGCGGGTATCTTATATAAAAGAGAGTCCACACGTAGCGCC7020.18124173826834106No Hit
GTATACATGCATACAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAA6970.17995084269662923No Hit
CATTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATT6730.1737545439524124No Hit
TTATTATCCAGAGCATGTGGTTAATCATTACTTCCAGACGAGACATTATT6600.17039821546596168No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC6390.16497645406477196No Hit
CCCATAGGTATTTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGGTG6280.16213648380700593No Hit
ACCTATGGGAGTGGGCCTCAGTCCGTTTCTCTTGGCTCAGTTTACTAGTG6130.15826379709187047No Hit
CATGTATACAAGCAAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC5880.1518093192333113No Hit
GGAACGATGTATACTTGCGGGAGAGCACGACAGAATTGTCAGTCCCGATG5790.14948570720423002No Hit
CATCATTCCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGG5790.14948570720423002No Hit
GGAACATATTGTACAAAAAATCAAAATGTGTTTTAGGAAACTTCCTGTAA5700.14716209517514872No Hit
GGAGTTGTGCAGGTTTTGCATGGTCCGGTGCTGGTTGTTGATGATCCTGG5680.146645736946464No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGCAGAGGA5680.146645736946464No Hit
ATTCTGAGCAGGGCTCACTGTTCCTGAACTGGAGCCACCAGCAGGAAAGT5660.14612937871777923No Hit
CCTGTAAACAGACCTATTGATTGGAAAGTATGTCAACGAATTGTGGGTCT5650.1458711996034369No Hit
GTGGATATCCTGCTTTAATGCCTTTGTATGCATGTATACAAGCAAAACAG5650.1458711996034369No Hit
ATACCACATCATCCATATAACTGAAAGCCAGACAGTGGGGGAAAGCCCTA5600.14458030403172506No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG5530.14277305023132847No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC5520.14251487111698613No Hit
CTTTTCGTTTTGTGAGGGTTTAAATGTATACCCAAAGACAAAAGAAAATT5510.14225669200264376No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTC5330.13760946794448117No Hit
GTATGGATCGGCAGAGGAGACACAAAGGTTCCACGCATGCGCTGATGGCC5290.1365767514871117No Hit
ATATTAACGTTGACATAGCTGACTACTAATTCCCTGGATGCTGGATCTTC5240.13528585591539988No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCATCAACAACC5210.13451131857237275No Hit
ATGTAATTGGGTGTTGGGGCACATTGCCACAGGAACATATTGTACAAAAA5170.13347860211500331No Hit
CCACCGCATGGCCTGAGGATGAGTGTCCCTTAGAGGTGGAGATAAGGGAG5080.13115499008592202No Hit
GGATAATAAGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTAACATT5070.13089681097157962No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTAT5050.1303804527428949No Hit
GTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGTACAATATGTT5050.1303804527428949No Hit
GCACATCTCCTGCTCAAGGAACCTCTATGTTTCCCTCATGTTGCTGTACA5020.12960591539986782No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGGCCG4920.12702412425644416No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAG4920.12702412425644416No Hit
ATGTATACTTGCGGGAGAGCACGACAGAATTGTCAGTCCCGATGAGTTTT4860.12547504957038996No Hit
GACCAGACCGTTGCCGAGCAACGGGGTAAAGGTTCAGATACTGTTTACTT4840.12495869134170523No Hit
CTGTAAGGGGCCCCACAAATTGCTTACATCTATCAACAATGTCCTCCTGC4810.12418415399867812No Hit
GTATCTGAACCTTTACCCCGTTGCTCGGCAACGGTCTGGTCTGTGCCAAG4780.12340961665565102No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTCTTCCAAAAGTAAGA4700.1213441837409121No Hit
GTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTTTTGTC4700.1213441837409121No Hit
CCTCTATGTTTCCCTCATGTTGCTGTACAAAACCTACGGACGGAAACTGC4690.12108600462656972No Hit
GAATACTGTCTCTGCCATATCGTCAATCTTATCGAAGACTGGGGACCCTG4690.12108600462656972No Hit
AAACAAAAAGATGGGGATATTCCCTTAACTTCATGGGATATGTAATTGGG4590.11850421348314608No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCTGCATATAGACCACCAAATGCC4580.1182460343688037No Hit
GGTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGCATAA4580.1182460343688037No Hit
GTCCAACTGATGATCGGGGAAGAATCCCAGGGGATTGGGGACAGAAAGAT4550.1174714970257766No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGGCACTCAGGCA4540.11721331791143424No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACGAGAAAAACCCC4480.11566424322538005No Hit
GAATACAGGTGCAGTTTCCGTCCGTAGGTTTTGTACAGCAACATGAGGGA4360.11256609385327165No Hit
GGATATCCACATTGCGTGAAAGGGGCGGCAAACCCCAAAAGACCCACAAT4360.11256609385327165No Hit
CTTCTGGACTATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATC4320.11153337739590219No Hit
GTAATTGGGTGTTGGGGCACATTGCCACAGGAACATATTGTACAAAAAAT4320.11153337739590219No Hit
CCTGTATTCCCATCCCATCATCTTGGGCTTTCGCAAAATACCTATGGGAG4300.11101701916721744No Hit
GCTCAGAATACTGTCTCTGCCATATCGTCAATCTTATCGAAGACTGGGGA4290.11075884005287509No Hit
GCTTTAATGCCTTTGTATGCATGTATACAAGCAAAACAGGCTTTTACTTT4260.10998430270984798No Hit
CAGTTGGACCCTGCATTCAAAGCCAACTCAGAAAATCCAGATTGGGACCT4210.10869340713813615No Hit
GGGTAAAGGTTCAGATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGA4190.10817704890945141No Hit
GATGCGATGTTCTCCATGTTCGGTACAGGGTCCCCAGTCTTCGATAAGAT4170.10766069068076668No Hit
GCCTAGCAGCCATGGGAATGATGTATACTTGCGGGAGAGCACGACAGAAT4160.10740251156642433No Hit
TCTATCAACAATGTCCTCCTGCAAATGAATGTCAGGAAAAGAAGGAGTTT4150.10714433245208195No Hit
CCCTTACAGTAAATGAAAACAGGAGACTAAAATTAATTATGCCTGCTAGG4150.10714433245208195No Hit
GATGTGATGTTCTCCATGTTCGGTACAGGGTCCCCAGTCTTCGATAAGAT4140.10688615333773958No Hit
ATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAA4120.10636979510905485No Hit
CATCACATCAGGACTCCTAGGACCCCTGCTCGTGTTACAGGCGGGGGTTT4110.1061116159947125No Hit
CCCCAACAGTCCCCCATGGGGAGGGATGAACCCTGGCCCGAATGCTCCCA4070.10507889953734303No Hit
CGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCCT4050.10456254130865829No Hit
GACGGGGAGTCCGCGTAAAGAGAGGTGCGCCCCGTGGTCGGTCGGTACAC4020.1037880039656312No Hit
GTGTACCGACCGACCACGGGGCGCACCTCTCTTTACGCGGACTCCCCGTC4020.1037880039656312No Hit
ATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGG4010.10352982485128882No Hit
GTATACATCATTCCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTGC3990.10301346662260409No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGCATAAA3990.10301346662260409No Hit
GGAGATGTGCAGGTTTTGCATGGTCCGGTGCTGGTTGTTGATGATCCTGG3980.10275528750826174No Hit
GTTCAGATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAA3960.10223892927957699No Hit
CAATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGG3910.10094803370786518No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)