FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488350

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488350
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences590972
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACACACAGTCTTTGA23210.39274280338154766No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA22760.3851282294254212No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC22380.37869814475135877No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA18220.3083056388458337No Hit
ATATAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTTTCCATGC14570.24654298342391853No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG13980.23655943090366377No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC12950.2191305171818631No Hit
GCTGTTCTGTGTTGGGGTGAGTTGATGAATCTGGCCACCTGGGTGGGAAG12830.217099964126896No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCGCAG12830.217099964126896No Hit
CTATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATA12670.21439256005360657No Hit
AGCTACTCTGTGTTGGGGTGAGTTGATGAATCTGGCCACCTGGGTGGGAA12390.2096546029253501No Hit
CAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTGTGGTG12200.20643956058831892No Hit
CAGAACAGCTTGCCTGAGTGCTGTATGGTGAGGTGAACAATGTTCCGGAG12120.2050858585516742No Hit
CCTGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC12080.2044090075333518No Hit
ATCCTAACCTTACTAAATATTTGCCCTTAGACAAAGGCATTAAACCGTAT11740.1986557738776118No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN11710.19814813561387004No Hit
GTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGC11650.19713285908638647No Hit
ATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATATT11620.19662522082264472No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT11440.19357939124019413No Hit
GCTACTCTGTGTTGGGGTGAGTTGATGAATCTGGCCACCTGGGTGGGAAG11290.1910411999214853No Hit
CATTTAAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT11250.19036434890316292No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACA11240.19019513614858233No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCCGCTTACAGACCACCAAATGCC10790.1825805621924558No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT10460.17699654129129636No Hit
CCCTAGCAGCCATGGAAAGGAGGTGTACTTCCGAGAGAGGACAACAGAGT9780.1654900739798163No Hit
GTCTAGACTTGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCAAG9780.1654900739798163No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA9510.1609213296061404No Hit
CATCCGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATT9030.1527991173862721No Hit
GGACCCCTAGAAGAAGAACTCCCTCGCCTCGCAGACGAAGGTCTCAATCG9010.15246069187711092No Hit
CTAGTGGACCTGCCTCGACATCTAACAACAGTAGTTTCCGGAAGTGTTGA8830.14941486229466033No Hit
GCCTAATCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC8790.14873801127633798No Hit
CCTAGTCTCCTCCCCCAACTCCTCCCAGTCTTTAAACACACAGTCTTTGA8640.14619981995762912No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA8520.14416926690266207No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG8410.14230792660227556No Hit
ATCAATATCTGAACCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGC8340.14112343732021146No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA8280.1401081607927279No Hit
GTCAACGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACAT8280.1401081607927279No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT8220.13909288426524438No Hit
CCGTAAGAGGGCCCACATATTGTTGACATCTGTCAATAATGTCCTCCTGT7860.13300122510034315No Hit
TTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACAC7680.12995539551789254No Hit
CCATTGATCCTTGTTGGGGTTGAAGTCCCAATCTGGATTGTTTGAGTTGG7540.1275864169537643No Hit
GATCACTGGCCAGAGGCAAATCAGGTAGGAGCGGGAGCATTCGGGCCAGG7520.12724799144460314No Hit
CTTATATAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTTTCCA7490.12674035318086138No Hit
CAGAGTAGCTTGCCTGAGTGCTGTATGGTGAGGTGAACAATGTTCCGGAG7480.1265711404262808No Hit
GGGTAAAGGTTCAGATATTGATTACGCAGAAAGGCCTTGTAAGTTGGCGA7330.12403294910757193No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA7250.12267924707092721No Hit
GGGCACATTGACAACAGTGCCAGCAGCACCTCCTCCTGCCTCCACCAATC7230.12234082156176605No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCTGATGGCC7220.12217160880718546No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTGTTTGCTG7170.1213255450342825No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACA7080.11980263024305719No Hit
GGTCCTAGGAATCCTGATGTTGTGTTCTCCATGTTCGGTGCAGGGTCCCC7060.11946420473389603No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT6950.11760286443350955No Hit
CTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATCATT6920.11709522616976777No Hit
ATTGACAACAGTGCCAGCAGCACCTCCTCCTGCCTCCACCAATCGGCAGT6920.11709522616976777No Hit
AACATTGACATAGCTGACTACTAATTCCCTGGATGCTGGGTCTTCCAAAT6860.11607994964228424No Hit
GTATACAGGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA6720.11371097107815598No Hit
CTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCTTG6710.11354175832357541No Hit
ATATGTTCCTGCGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA6660.11269569455067245No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT6660.11269569455067245No Hit
CTACTGTACCTGTCTTTAATCCCGAGTGGCAAACTCCCTCCTTTCCTCAC6590.11151120526860833No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT6540.11066514149570539No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAAA6530.1104959287411248No Hit
GGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCATTAAC6530.1104959287411248No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC6510.11015750323196362No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTTAAATGTATA6490.10981907772280243No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTCAGGCA6450.10914222670448008No Hit
GTCTTATATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT6420.10863458844073831No Hit
CCCAAGTGTCCTGGCCAAAATTCGCAGTCCCCAACCTCCAATCACTCACC6290.10643482263119064No Hit
GGATGGGGCTTGGCCATCGGCCATCAGCGCATGCGTGGAACCTTTGTGGC6270.10609639712202948No Hit
GTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATG6200.10491190783996533No Hit
ATCCTGAACATGCAGTTAATCATTACTTCAAAACTAGGCATTATTTACAT6180.10457348233080417No Hit
CCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAGTG6160.104235056821643No Hit
GTCTATTTACAGGCAATTTTCGAAAACATTGCTTGAGTTTTAGTACAATA6140.10389663131248181No Hit
GGGTCCTAGGAATCCTGATGTTGTGTTCTCCATGTTCGGTGCAGGGTCCC6120.10355820580332062No Hit
CTCTTACGGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG6090.10305056753957886No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG6080.10288135478499828No Hit
CTTGTATTCCCATCCCATCATCCTGGGCTTTCGCAAGATTCCTATGGGAG6060.1025429292758371No Hit
CCCACGTGTCCTGGCCAAAATTCGCAGTCCCCAACCTCCAATCACTCACC6030.10203529101209532No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGA5990.10135843999377298No Hit
CTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGAAGAGAAACTGTTCT5970.10102001448461179No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG5940.10051237622087002No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)