FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488351

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488351
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences590972
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC21760.36820695396736225No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA20590.3484090616814333No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA18460.31236674495576777No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG13980.23655943090366377No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC13260.22437611257386134No Hit
CTATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATA12490.21134673047115599No Hit
CCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACACACAGTCTTTGA12360.20914696466160834No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCGCAG12210.20660877334289948No Hit
CCTGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC11910.20153239070548182No Hit
CAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTGTGGTG11830.2001786886688371No Hit
GCTGTTCTGTGTTGGGGTGAGTTGATGAATCTGGCCACCTGGGTGGGAAG11790.1995018376505147No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN11710.19814813561387004No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACA11700.19797892285928942No Hit
ATCCTAACCTTACTAAATATTTGCCCTTAGACAAAGGCATTAAACCGTAT11640.1969636463318059No Hit
ATATAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTTTCCATGC11550.19544073154058061No Hit
AGCTACTCTGTGTTGGGGTGAGTTGATGAATCTGGCCACCTGGGTGGGAA11440.19357939124019413No Hit
ATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATATT11100.1878261575844541No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT10870.18393426422910053No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCCGCTTACAGACCACCAAATGCC10860.18376505147451996No Hit
GTCTAGACTTGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCAAG10800.1827497749470364No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT10710.1812268601558111No Hit
GCTACTCTGTGTTGGGGTGAGTTGATGAATCTGGCCACCTGGGTGGGAAG10600.17936551985542462No Hit
CATTTAAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT10520.17801181781877992No Hit
CAGAACAGCTTGCCTGAGTGCTGTATGGTGAGGTGAACAATGTTCCGGAG10230.17310464793594282No Hit
CTAGTGGACCTGCCTCGACATCTAACAACAGTAGTTTCCGGAAGTGTTGA9500.1607521168515598No Hit
GCCTAATCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC9440.15973684032407626No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT9390.15889077655117334No Hit
CATCCGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATT9370.15855235104201215No Hit
GTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGC9080.15364518115917505No Hit
CCCTAGCAGCCATGGAAAGGAGGTGTACTTCCGAGAGAGGACAACAGAGT9070.15347596840459446No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA8960.151614628104208No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG8750.1480611602580156No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA8690.14704588373053207No Hit
GGACCCCTAGAAGAAGAACTCCCTCGCCTCGCAGACGAAGGTCTCAATCG8350.14129265007479205No Hit
GTCAACGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACAT8180.13841603324692203No Hit
ATCAATATCTGAACCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGC8050.13621626743737436No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTGTTTGCTG8040.13604705468279377No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA7730.1308014592907955No Hit
CCGTAAGAGGGCCCACATATTGTTGACATCTGTCAATAATGTCCTCCTGT7670.12978618276331197No Hit
CCATTGATCCTTGTTGGGGTTGAAGTCCCAATCTGGATTGTTTGAGTTGG7550.1277556297083449No Hit
CTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCTTG7440.1258942894079584No Hit
GTCTTATATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT7410.12538665114421663No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG7210.12200239605260486No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCTGATGGCC7100.12014105575221838No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT7070.1196334174884766No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG7000.11844892820641248No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA6930.11726443892434836No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT6900.11675680066060659No Hit
GATCACTGGCCAGAGGCAAATCAGGTAGGAGCGGGAGCATTCGGGCCAGG6840.11574152413312305No Hit
CCTAGTCTCCTCCCCCAACTCCTCCCAGTCTTTAAACACACAGTCTTTGA6800.11506467311480072No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT6750.11421860934189776No Hit
CTACTGTACCTGTCTTTAATCCCGAGTGGCAAACTCCCTCCTTTCCTCAC6690.11320333281441422No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACA6620.1120188435323501No Hit
GGGTAAAGGTTCAGATATTGATTACGCAGAAAGGCCTTGTAAGTTGGCGA6600.11168041802318893No Hit
GGATGGGGCTTGGCCATCGGCCATCAGCGCATGCGTGGAACCTTTGTGGC6600.11168041802318893No Hit
GGGCACATTGACAACAGTGCCAGCAGCACCTCCTCCTGCCTCCACCAATC6570.11117277975944714No Hit
GGTCCTAGGAATCCTGATGTTGTGTTCTCCATGTTCGGTGCAGGGTCCCC6540.11066514149570539No Hit
GGGTCCTAGGAATCCTGATGTTGTGTTCTCCATGTTCGGTGCAGGGTCCC6540.11066514149570539No Hit
TTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACAC6530.1104959287411248No Hit
GTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCCTTT6530.1104959287411248No Hit
GTATACAGGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA6520.11032671598654421No Hit
GTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATG6490.10981907772280243No Hit
ATATGTTCCTGCGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA6450.10914222670448008No Hit
CAGAGTAGCTTGCCTGAGTGCTGTATGGTGAGGTGAACAATGTTCCGGAG6420.10863458844073831No Hit
CTTATATAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTTTCCA6400.10829616293157712No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTCAGGCA6320.10694246089493241No Hit
ATTGACAACAGTGCCAGCAGCACCTCCTCCTGCCTCCACCAATCGGCAGT6210.10508112059454593No Hit
AACATTGACATAGCTGACTACTAATTCCCTGGATGCTGGGTCTTCCAAAT6190.10474269508538477No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC6170.10440426957622358No Hit
GTCTATTTACAGGCAATTTTCGAAAACATTGCTTGAGTTTTAGTACAATA6160.104235056821643No Hit
CCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAGTG6100.10321978029415946No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAAA6050.1023737165212565No Hit
CGGTGGTTTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG6030.10203529101209532No Hit
ATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCCTTTCTGCGTA6010.10169686550293416No Hit
GTGTTGGGGTGAGTTGATGAATCTGGCCACCTGGGTGGGAAGTAATTTGG5980.10118922723919238No Hit
GTATTATCCTGAACATGCAGTTAATCATTACTTCAAAACTAGGCATTATT5970.10102001448461179No Hit
CTCTTACGGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG5940.10051237622087002No Hit
ATCCTGAACATGCAGTTAATCATTACTTCAAAACTAGGCATTATTTACAT5930.10034316346628945No Hit
CTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATCATT5930.10034316346628945No Hit
CTGCCTAATCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGT5920.10017395071170886No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)