FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002488353

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488353
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences336844
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGG18040.535559487477883No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN16620.49340347460545536No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACACCCGTG11780.3497167828430965No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATAGCTGAAAGTCCAA11610.3446699362316087No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAATACAAAGACC10700.31765446319364454No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC9440.2802484236026172No Hit
ACTCACAACTGTGTCAACAGCTCCACCTCCTGCCTCCACCAATCGGCAGG9050.268670363729204No Hit
GTTGTGAGTATGCCCTGAGCTTGAGGGCTCCACCCCAACAGTCCCCCATG8470.25145171058412796No Hit
CCTCCTGCATATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA8280.2458111173124651No Hit
CCTATTAACAATGTCCTCCCGCAAATGAATGTCAGGAAAAGAAGGAGTTT8250.24492049732220256No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATAACCACATTGTGTGAAA8050.2389830307204522No Hit
GTTAATAGGTGTAAACAATTTGTAGGCCCCCTCACAGTAAATGAAAACAG7950.236014297419577No Hit
TCCTCAGGCCATGCAGTGGAACTCCACCACGTTCCACCAAACTCTTCAAG7330.21760815095415087No Hit
CATGTATACAAGCTAAAAAGGCTTTCACTTTCTCGCCAACTTATAAGGCC6920.20543634442056263No Hit
TTATTATCCAGAGCATGTAGTTAATCATTACTTCCAGACGAGACATTATT6760.20068637113916232No Hit
GTTCAGAACACTGCCTCTTCCATATCGTCAATCTTATCGAAGACTGGGGA6670.19801451116837468No Hit
CCCTTAACTTCATGGGATATATAATTGGAGGTTGGGGCACCTTACCCCAG6410.1902958045860992No Hit
GGATAATAAGGTTTAATTCCTTTATCTAAGGGCAAATATTTAGTAACGTT6140.18228022467373622No Hit
GTTCTGAACAGGGTTCACTGTTCCTGAACTGGAGCCACCAGCAGGAAAGT6060.17990523803303607No Hit
ATGTAAGACCTTGGGCAGGGTCCGGTGGGCGTTCACGGTGGTCTCCATGC5810.17248340478084812No Hit
GCACCGGACCATGCAGAACCTGCACGACTCCTGCTCAAGGAACCTCTATG5730.17010841814014796No Hit
CATTTAAACCCTCATAAAACAAAAAGATGGGGCTACTCCCTTAACTTCAT5670.16832717815962286No Hit
GTCCGGTGCTGGTGGTTGATGATCCTGGAATTAGAGGACAAACGGGCAAC5570.16535844485874768No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAGGCGAAGTGCACACGGTCCG5460.16209283822778497No Hit
GGATAGAACCTAGCAGGCATGATCAATTTTAGTCTCCTGTTTTCATTTAC5440.16149909156760994No Hit
GCCTAGCAGCCATGGAAACGAAGTATATTTTCGGGAAAGAACGACAGAAT5430.16120221823752243No Hit
TCCCTATACAGAGCAGAGGCGGTGTCGAGAAGATCTCGAATAGAAGGAAA5370.1594209782569973No Hit
ATCCTAACGTTACTAAATATTTGCCCTTAGATAAAGGAATTAAACCTTAT5240.15556162496585957No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTTTTGGGGGC5200.1543741316455095No Hit
ACCTATGGGAGTGGGCCTCAGTCCGTTTCTCTTGGCTCAGTTTACTAGTG5200.1543741316455095No Hit
GTGTGAAAGGAGCAGCAAATCCTAAAAGACCCACAATTCGTTGACATACC5140.15259289166498438No Hit
CTCCAAAAGACACCAAATATTCTAGAACAGTTTCTCTTCCAAAAGTAAGA5130.15229601833489687No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCATCAACCACC5120.15199914500480935No Hit
TTCTAGAACAGTTTCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACA5080.15081165168445929No Hit
CCCATAGGTATTTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGGTG4970.14754604505349658No Hit
CTTATAAGGCCTTTCTAAGCAAACAATATCTGAACCTTTACCCCGTTGCT4930.14635855173314652No Hit
CGTTAGGATAGAACCTAGCAGGCATGATCAATTTTAGTCTCCTGTTTTCA4910.14576480507297146No Hit
GGTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGCATAA4860.14428043842253388No Hit
ACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCTGCATGGTCCGGTG4840.14368669176235885No Hit
GTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATAGC4830.14338981843227133No Hit
AGTTAAGGGAGTAGCCCCATCTTTTTGTTTTATGAGGGTTTAAATGTATA4790.14220232511192124No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTC4770.1416085784517462No Hit
CCTGTAAACAGGCCTATTGATTGGAAGGTATGTCAACGAATTGTGGGTCT4670.13863984515087102No Hit
GTCTTACATAAGCGGACTATTGGACTTTCAGCTATGTCAACGACCGACCT4650.138046098490696No Hit
CCTCTATGTTTCCCTCATGTTGCTGTACAAAACCTACGGATGGAAACTGC4630.13745235183052096No Hit
ATATAAAAGAGAAACAACACGTAGCGCCTCATTTTGCGGGTCACCATATT4520.13418674519955825No Hit
GTTCTAGAATATTTGGTGTCTTTTGGAGTGTGGATTCGCACTCCTCCTGC4430.13151488522877058No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCTGCATATAGACCACCAAATGCC4420.13121801189868307No Hit
GGGTAAAGGTTCAGATATTGTTTGCTTAGAAAGGCCTTATAAGTTGGCGA4390.13032739190842052No Hit
GCCTGAGGATGAGTGTCTCTTAGAGGCGGAGACAGGGGAGTAGGCTGCCT4390.13032739190842052No Hit
GTATGGATCGGCAGAGGAGACACAAAGGTTCCACGCATGCGCTGATGGCC4300.12765553193763285No Hit
CTCTTTGGAAGGCGGGTATCTTATATAAAAGAGAAACAACACGTAGCGCC4280.12706178527745782No Hit
TCCAAAGAGTATGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACA4220.1252805452969327No Hit
TCCTTGGACTCATAAGGTGGGAAACTTTACGGGGCTTTACTCTTCTACGG4220.1252805452969327No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG4200.12468679863675766No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTAT4190.12438992530667015No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG4180.12409305197658264No Hit
GTTTACACCTATTAACAATGTCCTCCCGCAAATGAATGTCAGGAAAAGAA4120.12231181199605752No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG4090.12142119200579497No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGCATAAA4060.12053057201553241No Hit
ATATAATTGGAGGTTGGGGCACCTTACCCCAGGAACATATTGTATTGAAA4040.11993682535535737No Hit
CCTGTATTCCCATCCCATCATCTTGGGCTTTCGCAAAATACCTATGGGAG3990.11845245870491979No Hit
CAATAGGCCTGTTTACAGGAAGTTTCCTAAAACATTGTTTGATTTTCAAT3970.11785871204474474No Hit
GTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCCGCCTGTAACACG3970.11785871204474474No Hit
CTTCAAAGACTGTGTGTTTACTGAGTGGGAGGAGTTGGGGGAGGAGATCA3920.11637434539430716No Hit
TCCTTTGTCTACGTCCCGTCAGCGCTGAATCCCGCGGACGACCCCTCTCG3920.11637434539430716No Hit
ATGTTAATATGGGCCTAAAAATCAGACAATTATTGTGGTTTCACATTTCC3910.11607747206421963No Hit
GTTTACAGGAAGTTTCCTAAAACATTGTTTGATTTTCAATACAATATGTT3890.1154837254040446No Hit
GTATGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACATGCTCTGG3810.11310873876334444No Hit
GTTCTATCCTAACGTTACTAAATATTTGCCCTTAGATAAAGGAATTAAAC3810.11310873876334444No Hit
CCTTTACCCCGTTGCTCGGCAACGGCCAGGTCTGTGCCAAGTGTTTGCTG3790.11251499210316943No Hit
GTACTTTCCTGCTGGTGGCTCCAGTTCAGGAACAGTGAACCCTGTTCAGA3780.11221811877308191No Hit
ATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCTA3780.11221811877308191No Hit
CAATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGG3760.11162437211290686No Hit
GTCCAATAGTCCGCTTATGTAAGACCTTGGGCAGGGTCCGGTGGGCGTTC3740.11103062545273182No Hit
GAATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGA3730.11073375212264432No Hit
GAATACAGGTGCAGTTTCCATCCGTAGGTTTTGTACAGCAACATGAGGGA3720.1104368787925568No Hit
CTTCTGGACTATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATC3720.1104368787925568No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGCAGAGGA3710.11014000546246928No Hit
CCTTATGAGTCCAAGGAATACTAACATTGAGATTCCCGAGATTGAGATCT3700.10984313213238175No Hit
ATCCAGAGCATGTAGTTAATCATTACTTCCAGACGAGACATTATTTACAT3690.10954625880229424No Hit
CCTCTGCACGTCGCATGGAGACCACCGTGAACGCCCACCGGACCCTGCCC3670.10895251214211919No Hit
ATATAACTGAAAGCCAGACAGTGGGGGAAAGCCCTACGAACCACTGAACA3650.10835876548194417No Hit
CCCCAACAGTCCCCCATGGGGTGGAGTGAATCCCGGCCCGAATGCTCCCA3640.10806189215185666No Hit
ATATGGAAGAGGCAGTGTTCTGAACAGGGTTCACTGTTCCTGAACTGGAG3630.10776501882176913No Hit
TATTAACATTGACATAGCTGACTACTAATTCCCTGGATGCTGGATCTTCC3610.10717127216159408No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAG3550.10539003218106899No Hit
GTCTACGTCCCGTCAGCGCTGAATCCCGCGGACGACCCCTCTCGGGGCCG3550.10539003218106899No Hit
CTTTTATATAAGATACCCGCCTTCCAAAGAGTATGTAAATAATGTCTCGT3540.10509315885098146No Hit
ATCTAAGGGCAAATATTTAGTAACGTTAGGATAGAACCTAGCAGGCATGA3520.10449941219080643No Hit
GTGTTGGGGCGAGTTGATGAATCTTGCTACCTGGGTGGGAAGTAATTTGG3520.10449941219080643No Hit
ATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGG3510.1042025388607189No Hit
ATTCTGTGTTGGGGCGAGTTGATGAATCTTGCTACCTGGGTGGGAAGTAA3510.1042025388607189No Hit
GTCCAAGGAATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGAC3450.1024212988801938No Hit
CCTTTCACACAATGTGGTTATCCTGCTTTAATGCCTTTATATGCATGTAT3440.10212442555010627No Hit
CTTCTACGGTACCTGGCTTTAATCCTGAATGGCAAACTCCTTCTTTTCCT3440.10212442555010627No Hit
GTACAAAACCTACGGATGGAAACTGCACCTGTATTCCCATCCCATCATCT3430.10182755222001877No Hit
GTACAGACTTGGCCCCCAAAACCACATCATCCATATAACTGAAAGCCAGA3420.10153067888993125No Hit
GGCCTATTGATTGGAAGGTATGTCAACGAATTGTGGGTCTTTTAGGATTT3410.10123380555984374No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGGCACTCAGGCA3390.10064005889966869No Hit
GTACAGGGTCCCCAGTCTTCGATAAGATTGACGATATGGAAGAGGCAGTG3390.10064005889966869No Hit
GTACCGAACATGGAGAACATCGCATCAGGACTCCTAGGACCCCTGCTCGT3370.10004631223949365No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)