FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002488355

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488355
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences366615
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA16440.44842682377971443No Hit
GTGTAAAGGGAGCGGCAAAGCCCAGAAGCCCCACAATTCGTTGACATACT14890.4061481390559579No Hit
TCCATAGAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATG14660.3998745277743682No Hit
CTCTATGGAAGGCTGGTATTTTATATAAGAGAGAAACCACTCGCAGCGCC13850.37778050543485675No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG13430.3663243457032582No Hit
CCCTTTACACAATGTGGTTACCCAGCATTAATGCCTTTGTATGCATGTAT13330.36359668862430616No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAGACC12780.34859457469006994No Hit
GTCAAGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGAACATCAACTACC10780.2940414331110293No Hit
CCTCCCGCTTACAGACCACAAAATGCCCCTATCTTATCAACACTTCCGGA10300.2809486791320595No Hit
TACCTTGACAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG9890.2697652851083562No Hit
GTAGTAAGCTATGTCAATGTTAATATGGGCCTAAAATTCAGACAACTATT9080.2476712627688447No Hit
CATTTAAACCCTACTAAAACTAAACGTTGGGGCTACTCCCTTAACTTCAT9070.2473984970609495No Hit
GGATATTATCAATAGATGTCAACAATATGTAGGCCCTCTTACAGTTAACG9000.2454891371056831No Hit
ATGTAAGACCTTGGGCAAGACTTGTCGGGCGTTCACGGTGGTCTCCATGC8450.2304870231714469No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC8290.2261227718451236No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA7850.21412108069773467No Hit
ATCCTAACCTTACCAAATATTTGCCCTTAGATAAAGGCATTAAACCTTAT7430.20266492096613614No Hit
GGTTAGGATAGAACCTAGCAGGCATAATCAATTTTAATCTTCTTTTTTCG7390.20157385813455533No Hit
CTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCTCTTTTCCTCAC7310.19939173247139372No Hit
ATTTCCGTCCGAAGGTTTTGTACAGCAACAAGAGGGAAACATAGAGGTTC7150.19502748114507046No Hit
GCTTACTACTGCTTCCCTGGATGCTGGGTCTTCCAAATTACTTCCCACCC7110.19393641831348962No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG6920.18875386986348075No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACCTTACCACAG6770.1846623842450527No Hit
GTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATGTGTTCAGG6620.18057089862662468No Hit
CTGTAAGAGGGCCTACATATTGTTGACATCTATTGATAATATCCTCTTTC6410.17484281876082539No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCCGCTTACAGACCACAAAATGCC6340.17293345880555896No Hit
GTATACATGCATACAAAGGCATTAATGCTGGGTAACCACATTGTGTAAAG6210.16938750460292132No Hit
CTCCATATTCGGTGCAGGGTCCCCAGTCCTCGAGAAGATTGACGATATGG6200.16911473889502612No Hit
GCACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG6180.1685692074792357No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG6060.16529601898449328No Hit
CATGTATACAAGCTAAGCAAGCTTTCACTTTCTCGCCAACTTACAAGGCC5880.1603862362423796No Hit
TCCTCAGGCCATGCAGTGGAATTCCAGCACATTCCACCAAGCTCTGCTAG5770.15738581345553238No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG5750.15684028203974196No Hit
CTCTTACAGTTAACGAAAAAAGAAGATTAAAATTGATTATGCCTGCTAGG5720.15602198491605634No Hit
GCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT5670.15465815637658034No Hit
GCAATAAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATCTGGCCAC5660.15438539066868512No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA5650.15411262496078992No Hit
GTGTAAACAATATCTGAACCTTTACCCCGTTGCTCGGCAACGGTCAGGTC5610.15302156212920912No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAAGATGATGGGATGGGAATACAAG5600.15274879642131392No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTAATGCTCG5550.1513849678818379No Hit
ATATAAGAGAGAAACCACTCGCAGCGCCTCATTTTGTGGGTCACCATATT5540.1511122021739427No Hit
ATTTATAGGAAGTTTCCGAAAACATTGTTTGATTTTGTGTACAATATGTT5490.1497483736344667No Hit
GTATAGGCCTCTCACTCTGGGATCTAGCAGAGCTTGGTGGAATGTGCTGG5470.14920284221867627No Hit
GGATACTAACATTGAGGTTCCCGAGATTGAGATCTTCTGCGACGCGGCGA5340.14565688801603863No Hit
GTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC5310.14483859089235301No Hit
ATTTACATACTCTATGGAAGGCTGGTATTTTATATAAGAGAGAAACCACT5280.1440202937686674No Hit
CCTTTACCCCGTTGCTCGGCAACGGTCAGGTCTTTGCCAAGTGTTTGCTG5280.1440202937686674No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA5230.14265646522919137No Hit
GTCACGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGAACATCAACTACC5160.14074710527392495No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGATTGCCGTG5100.13911051102655375No Hit
GCCTGAGGATGACTGTCTCTTAGGGGTGGAGAGATGGGAGTAGGCTGTCT5080.13856497961076333No Hit
ATATGTTCCTGTGGTAAGGTACCCCAACTTCCAATTACATATCCCATGAA5070.13829221390286814No Hit
GCCTAATCATCTCATGTTCATGTCCCACTGTTCAAGCCTCCAAGCTGTGC5010.1366556196554969No Hit
AATATGGAGAGCACCACATCAGGATTCCTAGGACCCCTGCTCGTGTTACA5000.13638285394760172No Hit
GACGCAAATTGCACTTGTATTCCCATCCCATCATCTTGGGCTTTCGCAAG4990.1361100882397065No Hit
CCTATAAATAGGCCTATTGATTGGAAAGTATGTCAACGAATTGTGGGGCT4990.1361100882397065No Hit
GTCTACGTCCCATCGGCGCTGAATCCCGCGGACGACCCGTCTCGGGGCCG4970.1355645568239161No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCTGATGGCC4960.13529179111602088No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCAATAAGGCA4950.1350190254081257No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGA4900.13365519686864968No Hit
ATGTAGGCCCTCTTACAGTTAACGAAAAAAGAAGATTAAAATTGATTATG4820.13147307120548804No Hit
ACTGCATGGCCTGAGGATGACTGTCTCTTAGGGGTGGAGAGATGGGAGTA4800.13092753978969765No Hit
GGATAGAACCTAGCAGGCATAATCAATTTTAATCTTCTTTTTTCGTTAAC4770.130109242666012No Hit
CAATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGG4720.128745414126536No Hit
CTATGGAAGGCTGGTATTTTATATAAGAGAGAAACCACTCGCAGCGCCTC4710.1284726484186408No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGAAGAGGA4690.12792711700285042No Hit
ATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGG4620.126017757047584No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT4620.126017757047584No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA4610.12574499133968878No Hit
CCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGACA4590.12519945992389836No Hit
CTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGAAGAGAAACTGTTCT4570.12465392850810797No Hit
ATGTTAATATGGGCCTAAAATTCAGACAACTATTGTGGTTTCACATTTCC4560.12438116280021276No Hit
GGATAATAAGGTTTAATGCCTTTATCTAAGGGCAAATATTTGGTAAGGTT4550.12410839709231754No Hit
ACATAGAGGTTCCTTGAGCAGGAATCGTGCAGGTCTTGCATGGTCCCGTG4540.12383563138442236No Hit
CCCCAAAAGATTGCCGTGTGGTGGAGTGAACCCTGGCCCGAAGGATCCCA4540.12383563138442236No Hit
ATCTGAACCTTTACCCCGTTGCTCGGCAACGGTCAGGTCTTTGCCAAGTG4540.12383563138442236No Hit
GGTTGGGGCTTGGCCATAGGCCATCAGCGCATGCGTGGAACCTTTGTGGC4500.12274456855284155No Hit
GCATTAATGCCTTTGTATGCATGTATACAAGCTAAGCAAGCTTTCACTTT4490.12247180284494634No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT4490.12247180284494634No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA4480.12219903713705112No Hit
ATTGACAACAGTACCTGCAGCGCCTCCTCCTGCCTCCACCAATCGGCAGT4470.12192627142915594No Hit
GTACAGTAGAAGAATAAAGCCCAGTAAAGTTTCCCACCTTGTGAGTCCAA4400.12001691147388951No Hit
GGATTAAAGACAGGTACAGTAGAAGAATAAAGCCCAGTAAAGTTTCCCAC4370.11919861435020389No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACA4360.11892584864230868No Hit
GTAGAAGAATAAAGCCCAGTAAAGTTTCCCACCTTGTGAGTCCAAGGGAT4320.11783478581072787No Hit
GAATATGGAGAGCACCACATCAGGATTCCTAGGACCCCTGCTCGTGTTAC4310.11756202010283266No Hit
GTGTTGGGGTGAGTTGATGAATCTGGCCACCTGGGTGGGAAGTAATTTGG4290.11701648868704226No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG4280.11674372297914706No Hit
TCCTTTGTCTACGTCCCATCGGCGCTGAATCCCGCGGACGACCCGTCTCG4270.11647095727125185No Hit
CTTTAATCCTGACTGGCAAACTCCCTCTTTTCCTCACATTCATTTGAAAG4240.11565266014756624No Hit
CTTCTGGACTGTCAAGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGAAC4180.11401606590019503No Hit
CCATATTCTTGGGAACAAGAGCTACAGCATGGGAGGTTGGTCTTCCAAAC4150.11319776877650942No Hit
GTCCAACTGGTGATCGGGAAAGAATCCCAGAGGATTTGGAACAGAAAGAT4090.1115611745291382No Hit
TACCGTGACAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG4090.1115611745291382No Hit
ATAATATCCTCTTTCAAATGAATGTGAGGAAAAGAGGGAGTTTGCCAGTC4080.111288408821243No Hit
GGCCTACATATTGTTGACATCTATTGATAATATCCTCTTTCAAATGAATG4070.11101564311334779No Hit
GGTATAGGCCTCTCACTCTGGGATCTAGCAGAGCTTGGTGGAATGTGCTG4070.11101564311334779No Hit
GGTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAA4040.11019734598966219No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGACTTGTCGGGCGTTC4010.10937904886597657No Hit
GTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCCGCCTGTAACACG3960.10801522032650056No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTGC3940.10746968891071015No Hit
GTTCAGGATAATAAGGTTTAATGCCTTTATCTAAGGGCAAATATTTGGTA3920.10692415749491975No Hit
CCCTTGGACTCACAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTG3920.10692415749491975No Hit
ACAATATGTAGGCCCTCTTACAGTTAACGAAAAAAGAAGATTAAAATTGA3920.10692415749491975No Hit
GATAATATCCTCTTTCAAATGAATGTGAGGAAAAGAGGGAGTTTGCCAGT3910.10665139178702454No Hit
GTTAATATGGGCCTAAAATTCAGACAACTATTGTGGTTTCACATTTCCTG3860.10528756324754851No Hit
AGTTAAGGGAGTAGCCCCAACGTTTAGTTTTAGTAGGGTTTAAATGTATA3800.10365096900017731No Hit
GGCCTATACCTTCCTGCTGGTGGCTCCAGTTCCGGAACAGTAATCCCTGT3790.10337820329228209No Hit
ATATGGGAGAGGCAGTAGTCGGAACAGGGATTACTGTTCCGGAACTGGAG3780.10310543758438688No Hit
GGTTATCGCTGGATGTGTCTGCGGCGTTTTATCATCTTCCTCTTCATCCT3780.10310543758438688No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT3780.10310543758438688No Hit
GTTCTTGAGTATTTGGTGTCTTTTGGAGTGTGGATTCGCACTCCTCCCGC3780.10310543758438688No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAATCCCTTTTTACC3740.10201437475280607No Hit
TTATTATCCTGAACACATAGTTAATCATTACTTCCAAACTAGGCATTATT3730.10174160904491086No Hit
CTTTTGGGGTGGAGCCCTCAGGCTCAGGGCATATTGACAACAGTACCTGC3720.10146884333701567No Hit
GTATGCATGTATACAAGCTAAGCAAGCTTTCACTTTCTCGCCAACTTACA3700.10092331192122526No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTCTA3700.10092331192122526No Hit
CTCTTATATAAAATACCAGCCTTCCATAGAGTATGTAAATAATGCCTAGT3670.10010501479753965No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)