FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488356

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488356
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences595832
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA18480.3101545402059641No Hit
CTTATATAGGATCCCAGCCTTCCAAAGAGTATGTAAATAATGTCGGGTTT16360.274574041004847No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC16140.2708817250500141No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN14840.24906349440781966No Hit
AAAACACCTCCGTGTGGGGGAGTGAACCCTGGCCCGAATGCTCCCACTCC14820.24872782932101664No Hit
GTCCTAGGAATCCTGATGTGATGTTCTCCATGTTCGGTGCAGGGTCCCCA14460.24268585775856283No Hit
GTCTTTGAAGTAGGCCTCAAGGTCGGTCGTTGACATTGCTGGGAGTCCAA13830.23211240752426857No Hit
GTATTTTCCTGCTGGTGGCTCCAGTTCAGGAACACTCAACCCTGTTCCAA13420.22523127324480724No Hit
CTATATAAGAGGGAAACTACACGTAGCGCCTCATTTTGCGGGTCACCATA13130.22036412948616388No Hit
GTATATGAACCTTTACCCCGTTGCCCGGCAACGGCCTGGTCTGTGCCAAG13070.21935713422575492No Hit
GTGTAAATGGAGCAGCAAAGCCCAAAAGACCCACAATTCTCTGACATACT12660.2124759999462936No Hit
ATATAATTGGAAGTTGGGGTTCATTGCCACAGGATCATATTGTACAAAAA12450.20895151653486216No Hit
GTTCATATACTGTTTACTCAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAA12020.20173471716859784No Hit
ATATGATCCTGTGGCAATGAACCCCAACTTCCAATTATATACCCCATGAA11480.1926717598249171No Hit
GGTGACCCCCCTAGAAAATTGAGAGAAGTCCACCACGAGTCTAGACTCTG11260.1889794438700842No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCCACAACAACC10740.1802521516132064No Hit
ATTATATACCCCATGAAATTCAGGGAATAACCCCATCTCTTTGTTTTGTT10640.17857382617919146No Hit
ACAGCAAGGTTCGTCCCCATGCCTTTGCGAGGTTTTGATGACCAACCTCC10370.17404234750735106No Hit
GGATCATATTGTACAAAAAATCAAACACTGTTTTAGAAAACTTCCTGTCA10150.17035003155251816No Hit
ATATAAGAGGGAAACTACACGTAGCGCCTCATTTTGCGGGTCACCATATT10130.17001436646571516No Hit
ATTGACCACATTGCCAGCAGTTCCTCCTCCTGCCTCCACCAATCGGCAGT10010.16800037594489722No Hit
GCTTTAATGCCTTTGTATGCATGTATTCAAGCGAAACAGGCTTTTACTTT9780.16414022744666282No Hit
CCTGCCAGGCTTGTGGCCATGGGTCCTTGACGGGGTTGAAGTCCCAATCT9110.15289544703876262No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCCGTCCG8910.1495387961707327No Hit
GTATAAAGGGACTCAAGATGTTGTACAGACTTGGCCCCCAGTACCACATC8720.14634997784610426No Hit
GCCTAATCATCTCTTGTACATGTCCCACTTTTCAAGCCTCCAAGCTGTGC8570.14383248969508183No Hit
GTAGAGGAATAAAGCCCAGTAAAATTTCCCACCTTATGAGTCCAAGGAAT8480.1423219968044684No Hit
CTCTTAGAGGTGGAGAGATGGGAGTAGGCTGCCTTCCTGACTGCCGATTG8370.14047583882705192No Hit
GCCAACCAGGTAGGAGTGGGAGCATTCGGGCCAGGGTTCACTCCCCCACA8350.14014017374024892No Hit
TTATTATCCTGATCATGTAGTTAATCATTACTTTCAAACCCGACATTATT8270.13879751339303698No Hit
GGGCACATTGACCACATTGCCAGCAGTTCCTCCTCCTGCCTCCACCAATC8260.13862968084963548No Hit
CCTTTCTGTTCCCAACCCTCTGGGATTCTTTCCCGATCATCAGTTGGACC8060.13527302998160556No Hit
ATTCAGAGCCAATTCAAACAATCCAGATTGGGACTTCAACCCCGTCAAGG8040.13493736489480257No Hit
CCATTTACACAATGTGGTTACCCTGCTTTAATGCCTTTGTATGCATGTAT8030.13476953235140107No Hit
TATTTAACCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACACACAG7990.13409820217779508No Hit
GTCTAGAGGCAAGTATTTAGTGCTGTTAGGATAAAATCTAGCAGGCATAA7970.1337625370909921No Hit
CTCTTGTACATGTCCCACTTTTCAAGCCTCCAAGCTGTGCCTTGGGTGGC7810.13107721639656816No Hit
CAATCATATACCCCATGAAATTCAGGGAATAACCCCATCTCTTTGTTTTG7650.12839189570214424No Hit
ATATAAGACCTTGGGCAGGATCTGAGGGGCGTTCACGGTGGTTTCCATGC7610.12772056552853825No Hit
GTTCCAAATATTGCCTCTCACATCTCGTCAATCTCCTCGAGGACTGGGGA7480.1255387424643188No Hit
GATCATGTAGTTAATCATTACTTTCAAACCCGACATTATTTACATACTCT7360.12352475194350085No Hit
TCCTCAGGCCATGCAGTGGAATTCCACAGCTTTCCACCAAGCTCTGCAAG7350.12335691940009935No Hit
CCTTTATACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA7310.12268558922649338No Hit
TTCCTCTACTGTCCCTATCTTTAATCCTGAATGGCAAACTCCTTCTTTTC7310.12268558922649338No Hit
CTGATGGGCGTTCACGGTGGTTTCCATGCAACGTGCAGAGGTGAAGCGAA7190.12067159870567541No Hit
TAGCCGCAGGATGAAGAGGAATATGATAAAACGCCGCAGACACATCCAGC7170.12033593361887245No Hit
CATTTAAACCCTAACAAAACAAAGAGATGGGGTTATTCCCTGAATTTCAT7100.11916110581506197No Hit
AGCCTGGCAGGTAGGAGTGGGAGCATTCGGGCCAGGGTTCACTCCCCCAC7080.11882544072825897No Hit
GTGTCAGCAATTTGTAGGCCCTCTCACTGTAAATGAAAAGAGAAGACTGA6840.11479745968662307No Hit
GGGGTCTTTTGGGGTGGAGCCCTCAGGCTCAGGGCACATTGACCACATTG6740.11311913425260813No Hit
GGGTAAAGGTTCATATACTGTTTACTCAGAAAGGCCTTGTAAGTTGGCGA6740.11311913425260813No Hit
GTATATAATTGGAAGTTGGGGTTCATTGCCACAGGATCATATTGTACAAA6660.11177647390539615No Hit
GTACCACATCATCCATATAGCTGAAAGCCAAACAGTGGGGGAAAGCCCTA6640.11144080881859315No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCTGATGGCC6560.11009814847138119No Hit
CTTTCGGAGTGTGGATTCGCACTCCTCCAGCTTATAGACCACCAAATGCC6530.10959465084117671No Hit
CTCTTTGGAAGGCTGGGATCCTATATAAGAGGGAAACTACACGTAGCGCC6520.10942681829777522No Hit
CCCATGGCCACAAGCCTGGCAGGTAGGAGTGGGAGCATTCGGGCCAGGGT6420.10774849286376025No Hit
CAAAACAACTCTATGTTTCCCTCATGTTGCTGTACAAAACCTACGGATGG6330.1062379999731468No Hit
GGTGCTCCCCCTAGAAAATTGAGAGAAGTCCACCACGAGTCTAGACTCTG6320.1060701674297453No Hit
GGTCCTAGGAATCCTGATGTGATGTTCTCCATGTTCGGTGCAGGGTCCCC6310.1059023348863438No Hit
GGAAAATACAGGCCCCTGACTCTGGGATCTTGCAGAGCTTGGTGGAAAGC6280.1053988372561393No Hit
GGAAACTACAGGCCCCTGACTCTGGGATCTTGCAGAGCTTGGTGGAAAGC6250.10489533962593484No Hit
CCCATGATGTAGCTCTTGTTCCCAAGAATATGGTGACCCGCAAAATGAGG6230.10455967453913183No Hit
ATATGAACCTTTACCCCGTTGCCCGGCAACGGCCTGGTCTGTGCCAAGTG6220.10439184199573033No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGGTCACCCGTG6170.10355267927872286No Hit
ACTAGATACAGCCTCAGCTCTGTATCGGGAAGCCTTAGAGTCTCCTGAGC6160.10338484673532136No Hit
GTATTAACATAATTGACTACTAGATCCCTGGATGCTGGATCTTCCAAATT6160.10338484673532136No Hit
TAGGTATTTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGGTGCAAT6110.10254568401831389No Hit
CTCCGAAAGAGACCAAATACTCAAGGACAGTTTCTCTTCCAAAAGTAAGG6100.10237785147491239No Hit
GGTTTTGCAGGGTCCCGTGCTGGTTGTTGTGGATCCTGGAATTAGAGGAC6040.10137085621450342No Hit
CTACCTGATCAGGATAATAAGGTTTAATCCCTTTGTCTAGAGGCAAGTAT6020.10103519112770042No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)