FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488357

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488357
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences595832
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA24300.40783308046563455No Hit
CTTATATAGGATCCCAGCCTTCCAAAGAGTATGTAAATAATGTCGGGTTT16340.274238375918044No Hit
GTCCTAGGAATCCTGATGTGATGTTCTCCATGTTCGGTGCAGGGTCCCCA15350.25762295412129593No Hit
GTATATGAACCTTTACCCCGTTGCCCGGCAACGGCCTGGTCTGTGCCAAG15070.252923642906054No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC14980.25141315001544057No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN14710.2468816713436002No Hit
GTATTTTCCTGCTGGTGGCTCCAGTTCAGGAACACTCAACCCTGTTCCAA14100.23664388619610896No Hit
GTGTAAATGGAGCAGCAAAGCCCAAAAGACCCACAATTCTCTGACATACT13260.2225459525503833No Hit
GTTCATATACTGTTTACTCAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAA12670.2126438324896951No Hit
ATATAATTGGAAGTTGGGGTTCATTGCCACAGGATCATATTGTACAAAAA12530.21029417688207416No Hit
AAAACACCTCCGTGTGGGGGAGTGAACCCTGGCCCGAATGCTCCCACTCC12450.20895151653486216No Hit
CTATATAAGAGGGAAACTACACGTAGCGCCTCATTTTGCGGGTCACCATA12290.20626619584043823No Hit
GTCTTTGAAGTAGGCCTCAAGGTCGGTCGTTGACATTGCTGGGAGTCCAA11290.18948294150028866No Hit
ATATGATCCTGTGGCAATGAACCCCAACTTCCAATTATATACCCCATGAA10760.1805878167000094No Hit
GGTGACCCCCCTAGAAAATTGAGAGAAGTCCACCACGAGTCTAGACTCTG10510.17639200311497202No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCCACAACAACC10190.17102136172612414No Hit
ATATAAGAGGGAAACTACACGTAGCGCCTCATTTTGCGGGTCACCATATT9970.16732904577129123No Hit
GCTTTAATGCCTTTGTATGCATGTATTCAAGCGAAACAGGCTTTTACTTT9830.1649793901636703No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCCGTCCG9540.16011224640502694No Hit
CTCTTAGAGGTGGAGAGATGGGAGTAGGCTGCCTTCCTGACTGCCGATTG9510.15960874877482242No Hit
GGATCATATTGTACAAAAAATCAAACACTGTTTTAGAAAACTTCCTGTCA9400.15776259079740598No Hit
GCCTAATCATCTCTTGTACATGTCCCACTTTTCAAGCCTCCAAGCTGTGC9390.15759475825400449No Hit
ATTATATACCCCATGAAATTCAGGGAATAACCCCATCTCTTTGTTTTGTT8900.1493709636273312No Hit
CTCTTGTACATGTCCCACTTTTCAAGCCTCCAAGCTGTGCCTTGGGTGGC8540.14332899206487734No Hit
TTATTATCCTGATCATGTAGTTAATCATTACTTTCAAACCCGACATTATT8500.14265766189127135No Hit
ACAGCAAGGTTCGTCCCCATGCCTTTGCGAGGTTTTGATGACCAACCTCC8300.13930101102324144No Hit
GTATAAAGGGACTCAAGATGTTGTACAGACTTGGCCCCCAGTACCACATC8250.13846184830623398No Hit
GTCTAGAGGCAAGTATTTAGTGCTGTTAGGATAAAATCTAGCAGGCATAA8130.13644785778541602No Hit
ATTGACCACATTGCCAGCAGTTCCTCCTCCTGCCTCCACCAATCGGCAGT8090.13577652761181003No Hit
CCTTTCTGTTCCCAACCCTCTGGGATTCTTTCCCGATCATCAGTTGGACC7990.13409820217779508No Hit
GTATATAATTGGAAGTTGGGGTTCATTGCCACAGGATCATATTGTACAAA7900.1325877092871816No Hit
GTTCCAAATATTGCCTCTCACATCTCGTCAATCTCCTCGAGGACTGGGGA7890.1324198767437801No Hit
GCCAACCAGGTAGGAGTGGGAGCATTCGGGCCAGGGTTCACTCCCCCACA7690.1290632258757502No Hit
CCATTTACACAATGTGGTTACCCTGCTTTAATGCCTTTGTATGCATGTAT7680.1288953933323487No Hit
ATTCAGAGCCAATTCAAACAATCCAGATTGGGACTTCAACCCCGTCAAGG7400.12419608211710682No Hit
GTAGAGGAATAAAGCCCAGTAAAATTTCCCACCTTATGAGTCCAAGGAAT7400.12419608211710682No Hit
TTCCTCTACTGTCCCTATCTTTAATCCTGAATGGCAAACTCCTTCTTTTC7320.12285342176989487No Hit
CCTGCCAGGCTTGTGGCCATGGGTCCTTGACGGGGTTGAAGTCCCAATCT7300.12251775668309188No Hit
GATCATGTAGTTAATCATTACTTTCAAACCCGACATTATTTACATACTCT7240.12151076142268291No Hit
CATTTAAACCCTAACAAAACAAAGAGATGGGGTTATTCCCTGAATTTCAT7150.12000026853206944No Hit
GTACCACATCATCCATATAGCTGAAAGCCAAACAGTGGGGGAAAGCCCTA6980.117147115294244No Hit
CCTTTATACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA6950.11664361766403954No Hit
GGGCACATTGACCACATTGCCAGCAGTTCCTCCTCCTGCCTCCACCAATC6910.11597228749043353No Hit
GGTCCTAGGAATCCTGATGTGATGTTCTCCATGTTCGGTGCAGGGTCCCC6820.11446179459982009No Hit
TCCTCAGGCCATGCAGTGGAATTCCACAGCTTTCCACCAAGCTCTGCAAG6760.11345479933941112No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGGTCACCCGTG6720.11278346916580512No Hit
CTCCGAAAGAGACCAAATACTCAAGGACAGTTTCTCTTCCAAAAGTAAGG6690.11227997153560065No Hit
ATATGAACCTTTACCCCGTTGCCCGGCAACGGCCTGGTCTGTGCCAAGTG6670.11194430644879765No Hit
CTCTTTGGAAGGCTGGGATCCTATATAAGAGGGAAACTACACGTAGCGCC6510.1092589857543737No Hit
ATTTACATACTCTTTGGAAGGCTGGGATCCTATATAAGAGGGAAACTACA6300.10573450234294231No Hit
CAATCATATACCCCATGAAATTCAGGGAATAACCCCATCTCTTTGTTTTG6300.10573450234294231No Hit
CTTTAATCCTGAATGGCAAACTCCTTCTTTTCCTAAAATTCATTTACATG6270.10523100471273783No Hit
GGGTAAAGGTTCATATACTGTTTACTCAGAAAGGCCTTGTAAGTTGGCGA6230.10455967453913183No Hit
AGCTGGAGGAGTGCGAATCCACACTCCGAAAGAGACCAAATACTCAAGGA6190.10388834436552585No Hit
GTGTCAGCAATTTGTAGGCCCTCTCACTGTAAATGAAAAGAGAAGACTGA6180.10372051182212436No Hit
GGGGTCTTTTGGGGTGGAGCCCTCAGGCTCAGGGCACATTGACCACATTG6170.10355267927872286No Hit
CTTGTACATGTCCCACTTTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTT6160.10338484673532136No Hit
AGCCTGGCAGGTAGGAGTGGGAGCATTCGGGCCAGGGTTCACTCCCCCAC6140.10304918164851838No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCTGATGGCC6020.10103519112770042No Hit
GGAAACTACAGGCCCCTGACTCTGGGATCTTGCAGAGCTTGGTGGAAAGC6000.10069952604089745No Hit
ACTAGATACAGCCTCAGCTCTGTATCGGGAAGCCTTAGAGTCTCCTGAGC5990.10053169349749594No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)