FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002488358

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488358
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences374887
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCCAGCTTATAGACCACCAAATGCCCCTATCCTATCAACACTTCCGGA25070.668734845433424No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC21050.5615025327632059No Hit
CGGTAGTTCCCCCTAGAAAATTGAGAGAAGTCCACCACGAGTCTAGACTC20680.5516328920448028No Hit
ATATCTCCCTGCTGGTGGCTCCAGTTCAGGAACAGTAAACCCTGTTCCGA19230.5129545703105203No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACTACACATAGCGCC17060.455070461232318No Hit
GGGTCCTAGGAATCCTGATGTGATGTTCTCCATGTTCAGTGCAGGGTCCC15200.4054555105938589No Hit
GAATCAGAGGACAAACGGGCAACATACCTTGATAGTCCAGAAGAACCAAC15020.40065406375787904No Hit
TCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAACAGTCTTTGA14600.3894506878072592No Hit
CCTCCTGTCCTCCAACTTGTCCTGGTTATCGCTGGATGTGTCTGCGGCGT14120.3766468295779795No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC13620.3633094772558131No Hit
GATTTAGATCTTCTGCGACGCGGCGATTGAGACCTTCGTCTGCGAGGCGA12570.33530103737926364No Hit
TCCATAGAGTGTGTAAATAGTGTCTAGTTTGGAAGTAATGATTAACTAGA11710.3123607913851374No Hit
CTACTGTACCTGTCTTTAATCCTCATTGGAAAACACCATCTTTTCCTAAT11090.295822474505651No Hit
ATCTCAATCTCGGGAATCTCAATGTTAGTATTCCTTGGACTCATAAGGTG10850.2894205453910112No Hit
GGCACCACCGTGTGTCTTGGCCAAAATTCGCAGTCCCCAACCTCCAATCA10830.28888705129812453No Hit
GGGATATATAGGCTTCTCACTCTGGGATCTTGCAGAGTTTGGTGGAAGGT10300.27474945783662813No Hit
CTCTAATTCCAGGATCCTCAACCACCAGCACGGGACCATGCCGCACCTGC9850.26274584074667834No Hit
GTGGTGCCCCCTAGAAAATTGAGAGAAGTCCACCACGAGTCTAGACTCTG9810.261678852560905No Hit
GTCTAGTTTGGAAGTAATGATTAACTAGATGTTCTGGATAATAAGGTTTA9470.25260945298183185No Hit
GGCTTTCACTTTCTCGCCAACTTACAAGGCCTTTCTGTGTAAACAATACC9200.245407282727862No Hit
GCCTAGTCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC9150.24407354749564533No Hit
GCACCACCGTGTGTCTTGGCCAAAATTCGCAGTCCCCAACCTCCAATCAC9100.24273981226342872No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACCTTCCACCAAACTCTGCAAG9090.24247306521698536No Hit
CTATTTACACACTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACTA8970.23927210065966548No Hit
GTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTAGGACATG8960.23900535361322212No Hit
GCCTAATCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC8850.2360711361023455No Hit
GACAAGAGGTTGGTGAGTGATTGGAGGTTGGGGACTGCGAATTTTGGCCA8620.22993595403414893No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGCACCACCGTG8420.2246010131052824No Hit
GTAGTTCCCCCTAGAAAATTGAGAGAAGTCCACCACGAGTCTAGACTCTG8400.22406751901239574No Hit
AGATTCGTTAGATCTCCCCAGCAAAGAATTGCTTGCCTGAGTGCAGTATG8150.2173988428513125No Hit
AGCTGGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATACTCTATAA8140.2171320958048692No Hit
GTAAAAAGGGACTCAAGATGCTGTACAGACTTGGCCCCCAATACCACATC8040.21446462534043592No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA7980.21286414306177595No Hit
TCCATAGAGTGTGTAAATAGTGTCTGGTTTGGAAGTAATGATTAACTAGA7600.20272775529692946No Hit
ATATAAGAGAGAAACTACACATAGCGCCTCATTTTGTGGGTCACCATATT7370.19659257322873291No Hit
GTATAAAGGGACTCAAGATGCTGTACAGACTTGGCCCCCAATACCACATC7110.1896571500212064No Hit
CTCTCTCGTCCCCTTCTCCGTCTGCCGTTCCGACCGACCACGGGGCGCAC7040.1877899206961031No Hit
GGGTCTCACCCCACCGCACGGAGGCCTTTTGGGGTGGAGCCCTCAGGCTC6960.18565594432455645No Hit
AAACCAGACACTATTTACACACTCTATGGAAGGCGGGTATATTATATAAG6660.17765353293125663No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCAGAGAGTCCAA6550.17471931542038No Hit
ACATAGAGGTTCCTTGAGCAGTAGTCATGCAGGTGCGGCATGGTCCCGTG6460.17231859200239005No Hit
GATTGAGATCTTCTGCGACGCGGCGATTGAGACCTTCGTCTGCGAGGCGA6380.17018461563084342No Hit
CATTTACACCAAGACATCATCAAAAAATGTGAACAGTTTGTAGGCCCACT6330.16885088039862678No Hit
GATCCAGCATCTAGAGACCTAGTAGTCAGTTATGTCAACAATAATGTGGG6320.16858413335218345No Hit
CTCTATAACTGTTTCTCTTCCAAAGGTGAGACAAGAAATGTGAAACCACA6300.16805063925929678No Hit
CCCTTATCCAATGGTAAATATTTGGTAACCTTTGGATAAAACCTAGCAGG6250.16671690402708017No Hit
GTTATAGAGTATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCAGC6180.16484967470197687No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA6010.1603149749124403No Hit
GCCTGAGGATGAGTGTTTCTCAAAGGTGGAGACAGCGGGGTAGGCTGCCT5890.15711401035512035No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC5860.15631376921579035No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG5800.15471328693713038No Hit
GTTTGGTGGAAGGTTGTGGAGTTCCACTGCATGGCCTGAGGATGAGTGTT5740.15311280465847044No Hit
GGGCACCACCGTGTGTCTTGGCCAAAATTCGCAGTCCCCAACCTCCAATC5720.15257931056558377No Hit
TTATATAAGAGAGAAACTACACATAGCGCCTCATTTTGTGGGTCACCATA5710.15231256351914044No Hit
GTAGAAGAATAAAGCCCAGTAAAGTTCCCCACCTTATGAGTCCAAGGAAT5620.1499118401011505No Hit
GGTATAAAGGGACTCAAGATGCTGTACAGACTTGGCCCCCAATACCACAT5570.14857810486893383No Hit
CCTCTTGTCCTCCAACTTGTCCTGGTTATCGCTGGATGTGTCTGCGGCGT5560.14831135782249052No Hit
GTGTAAATAGTGTCTAGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT5550.1480446107760472No Hit
CAGTAAACCCTGTTCCGACTACTGCCTCTCCCTTATCGTCAATCTTCTCG5510.14697762259027386No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT5410.14431015212584059No Hit
CTTTAGGACATGGACATTGACCCTTATAAAGAATTTGGAGCTTCCGTGGA5400.14404340507939725No Hit
GGATAGGGGCATTTGGTGGTCTATAAGCTGGAGGAGTGCGAATCCACACT5370.14324316394006725No Hit
ATCTAAATCTCGGGAATCTCAATGTTAGTATTCCTTGGACTCATAAGGTG5350.14270966984718061No Hit
GTGTAAATGTATATTAGGAAAAGATGGTGTTTTCCAATGAGGATTAAAGA5330.14217617575429395No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG5310.14164268166140728No Hit
GTGTGAAAGGGGCAGCAAAACCTAAAAGACCCACAATTCGTTGACATACT5270.140575693475634No Hit
CTTTATACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAA5230.13950870528986067No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGAAGAGGA5120.13657448777898407No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTCCGGGAGAGGACAACAGAGT5110.13630774073254073No Hit
GGATAAGGGTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACT5000.13337352322166413No Hit
ACCCAGCCCGAATGCTCCAGCTCCTACCTTGTTGGCGTCTGGCCAGGTGT4990.1331067761752208No Hit
CCAATTGGATCCAGCCTTCAGAGCAAACACCGCAAATCCAGATTGGGACT4980.13284002912877746No Hit
CTTTTTACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAA4940.13177304094300415No Hit
CATTTAAACCCTAACAAAACAAAGAGATGGGGTTACTCTCTAAATTTCAT4920.1312395468501175No Hit
GGTCCCCTAGAAGAAGAACTCCCTCGCCTCGCAGACGAAGGTCTCAATCG4890.13043930571078752No Hit
GTCTCCTGAGCATTGTTCACCTCACCATACTGCACTCAGGCAAGCAATTC4890.13043930571078752No Hit
GGGGAACTACCGTGTGTCTTGGCCAAAATTCGCAGTCCCCAACCTCCAAT4760.12697159410702427No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTTTGT4720.12590460592125094No Hit
GAACAAATGGCACTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCAC4710.1256378588748076No Hit
GGTAAAAAGGGACTCAAGATGCTGTACAGACTTGGCCCCCAATACCACAT4710.1256378588748076No Hit
ATATATCCCTGCTGGTGGCTCCAGTTCAGGAACAGTAAACCCTGTTCCGA4690.12510436478192097No Hit
GTTTGTAGGCCCACTCACAGTTAATGAGAAAAGAAGGTTGCAATTGATTA4660.12430412364259097No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC4660.12430412364259097No Hit
AGTTTAGGCAACTTTTGTGGTTTCACATTTCTTGTCTCACCTTTGGAAGA4610.12297038841037433No Hit
GGGGCACCACCGTGTGTCTTGGCCAAAATTCGCAGTCCCCAACCTCCAAT4610.12297038841037433No Hit
ACTACACATAGCGCCTCATTTTGTGGGTCACCATATTCTTGGGAACAAGA4570.12190340022460101No Hit
GTGTAAACAATACCTGAACCTTTACCCCGTTGCCCGGCAACGGCCAGGTC4540.12110315908527103No Hit
CGGTGGTGCCCCCTAGAAAATTGAGAGAAGTCCACCACGAGTCTAGACTC4530.1208364120388277No Hit
AAACTACACATAGCGCCTCATTTTGTGGGTCACCATATTCTTGGGAACAA4500.12003617089949771No Hit
GGGTTTACTGTTCCTGAACTGGAGCCACCAGCAGGGAGATATAGGCCTCT4500.12003617089949771No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA4460.1189691827137244No Hit
CTCTGGGATCTTGCAGAGTTTGGTGGAAGGTTGTGGAGTTCCACTGCATG4450.11870243566728107No Hit
GGATAACCACATTGTGTGAAAGGGGCAGCAAAACCTAAAAGACCCACAAT4410.11763544748150775No Hit
GTATGTCAACGAATTGTGGGTCTTTTAGGTTTTGCTGCCCCTTTCACACA4380.11683520634217777No Hit
GCACGGGACCATGCCGCACCTGCATGACTACTGCTCAAGGAACCTCTATG4380.11683520634217777No Hit
GTAGTCATGCAGGTGCGGCATGGTCCCGTGCTGGTGGTTGAGGATCCTGG4380.11683520634217777No Hit
GAATACAGGTGCAATTTCCGTCCGAAGGTTTGGTACAGCAACAGGAGGGA4340.11576821815640445No Hit
GATACATAGAGGTTCCTTGAGCAGTAGTCATGCAGGTGCGGCATGGTCCC4320.1152347240635178No Hit
GTGTTGATAGGATAGGGGCATTTGGTGGTCTATAAGCTGGAGGAGTGCGA4280.11416773587774448No Hit
GTACCAAACCTTCGGACGGAAATTGCACCTGTATTCCCATCCCATCATCC4190.11176701245975455No Hit
GTTTAATACCCTTATCCAATGGTAAATATTTGGTAACCTTTGGATAAAAC4170.11123351836686789No Hit
ACACATAGCGCCTCATTTTGTGGGTCACCATATTCTTGGGAACAAGATCT4130.11016653018109457No Hit
GCCTACCCCGCTGTCTCCACCTTTGAGAAACACTCATCCTCAGGCCATGC4120.10989978313465125No Hit
GGTCTGGAGCAAGCATTATCGGGACTGATAACTCTGTTGTCCTCTCCCGG4100.10936628904176458No Hit
TAACCACATTGTGTGAAAGGGGCAGCAAAACCTAAAAGACCCACAATTCG4090.10909954199532126No Hit
ATGTAAGACCTTGGGCAAGCTTTGGTGGGCGTTCACGGTGGTCTCCATGC4080.10883279494887793No Hit
CATTAATTCCCCCCAGCAAAGAATTGCTTGCCTGAGTGCAGTATGGTGAG4060.10829930085599128No Hit
GTACAGTAGAAGAATAAAGCCCAGTAAAGTTCCCCACCTTATGAGTCCAA4050.10803255380954795No Hit
GTGAAACCCAGCCCGAATGCTCCAGCTCCTACCTTGTTGGCGTCTGGCCA4040.10776580676310461No Hit
CCTCTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGC4030.1074990597166613No Hit
TGCTTGATCTTCCAAATTACCACCCACCCAGGTAGCTAGATTCGTTAGAT4030.1074990597166613No Hit
AATTCCCCCCAGCAAAGAATTGCTTGCCTGAGTGCAGTATGGTGAGGTGA4020.10723231267021796No Hit
TCCTTGAGCAGTAGTCATGCAGGTGCGGCATGGTCCCGTGCTGGTGGTTG4000.1066988185773313No Hit
GAATTAGAGGACAAACGGGCAACATACCTTGATAGTCCAGAAGAACCAAC4000.1066988185773313No Hit
CGAGCAGGGGTCCTAGGAATCCTGATGTGATGTTCTCCATGTTCAGTGCA3990.10643207153088798No Hit
CCTTTATACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA3980.10616532448444464No Hit
CTCATAAGGTGGGGAACTTTACTGGGCTTTATTCTTCTACTGTACCTGTC3970.10589857743800131No Hit
CCCCTATCCTATCAACACTTCCGGAGACTACTGTTGTTAGACGACGAGGC3960.10563183039155799No Hit
CCTGTATTCCCATCCCATCATCCTGGGCTTTCGGAAAATTCCTATGGGAG3940.10509833629867132No Hit
TCCTTGGACTCATAAGGTGGGGAACTTTACTGGGCTTTATTCTTCTACTG3940.10509833629867132No Hit
GGGGAGATCTAACGAATCTAGCTACCTGGGTGGGTGGTAATTTGGAAGAT3920.10456484220578467No Hit
GTGTAGTTTCTCTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAA3920.10456484220578467No Hit
CTACAAACTTTGCCAGCAAATCCGCCTCCTGCCTCCACCAATCGCCAGTC3900.10403134811289802No Hit
ATGTGGTTATCCTGCTTTAATGCCCTTGTATGCTTGTATTCAATCTAAGC3890.10376460106645469No Hit
CAAGCTTTGGTGGGCGTTCACGGTGGTCTCCATGCGACGTGCAGAGGTGA3890.10376460106645469No Hit
CCGAAAGACACCAAATACTCTATAACTGTTTCTCTTCCAAAGGTGAGACA3870.10323110697356803No Hit
CTCTCGTCCCCTTCTCCGTCTGCCGTTCCGACCGACCACGGGGCGCACCT3850.10269761288068137No Hit
GTGTAAATAGTGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT3780.10083038355557807No Hit
GGTGAAACCCAGCCCGAATGCTCCAGCTCCTACCTTGTTGGCGTCTGGCC3770.10056363650913475No Hit
GTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACT3760.10029688946269144No Hit
CTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAGTGTCTAGTTT3750.10003014241624811No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)