FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002488359

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488359
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences374887
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCCAGCTTATAGACCACCAAATGCCCCTATCCTATCAACACTTCCGGA24120.6433938760213077No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC20510.5470981922552662No Hit
ATATCTCCCTGCTGGTGGCTCCAGTTCAGGAACAGTAAACCCTGTTCCGA18280.48761360089840405No Hit
CGGTAGTTCCCCCTAGAAAATTGAGAGAAGTCCACCACGAGTCTAGACTC17360.4630728726256178No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACTACACATAGCGCC14210.37904755299596943No Hit
CCTCCTGTCCTCCAACTTGTCCTGGTTATCGCTGGATGTGTCTGCGGCGT13700.3654434536273597No Hit
GAATCAGAGGACAAACGGGCAACATACCTTGATAGTCCAGAAGAACCAAC13660.36437646544158636No Hit
GATTTAGATCTTCTGCGACGCGGCGATTGAGACCTTCGTCTGCGAGGCGA13150.3507723660729766No Hit
GGGTCCTAGGAATCCTGATGTGATGTTCTCCATGTTCAGTGCAGGGTCCC13000.3467711603763267No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC12100.3227639261964272No Hit
GTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTAGGACATG11230.29955693315585763No Hit
CTACTGTACCTGTCTTTAATCCTCATTGGAAAACACCATCTTTTCCTAAT11200.29875669201652766No Hit
TCCATAGAGTGTGTAAATAGTGTCTAGTTTGGAAGTAATGATTAACTAGA10690.2851525926479179No Hit
GCCTAGTCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC10420.27795042239394807No Hit
ATCTCAATCTCGGGAATCTCAATGTTAGTATTCCTTGGACTCATAAGGTG10340.2758164460224014No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACCTTCCACCAAACTCTGCAAG9640.25714415277136843No Hit
GCACCACCGTGTGTCTTGGCCAAAATTCGCAGTCCCCAACCTCCAATCAC9200.245407282727862No Hit
CTCTAATTCCAGGATCCTCAACCACCAGCACGGGACCATGCCGCACCTGC9150.24407354749564533No Hit
GCCTAATCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC9080.24220631817054206No Hit
GGGATATATAGGCTTCTCACTCTGGGATCTTGCAGAGTTTGGTGGAAGGT9010.24033908884543878No Hit
GTCTAGTTTGGAAGTAATGATTAACTAGATGTTCTGGATAATAAGGTTTA8980.23953884770610878No Hit
GGCACCACCGTGTGTCTTGGCCAAAATTCGCAGTCCCCAACCTCCAATCA8640.2304694481270356No Hit
GTGGTGCCCCCTAGAAAATTGAGAGAAGTCCACCACGAGTCTAGACTCTG8550.22806872470904566No Hit
CTATTTACACACTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACTA8460.22566800129105571No Hit
CTCTCTCGTCCCCTTCTCCGTCTGCCGTTCCGACCGACCACGGGGCGCAC8450.22540125424461238No Hit
GAATCTTTCCACCAGCAATCCTCTGGGATTCTTTCCCGACCACCAATTGG8440.22513450719816908No Hit
GACAAGAGGTTGGTGAGTGATTGGAGGTTGGGGACTGCGAATTTTGGCCA8210.21899932512997247No Hit
TCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAACAGTCTTTGA8210.21899932512997247No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA8150.2173988428513125No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGCACCACCGTG8030.21419787829399262No Hit
GGCTTTCACTTTCTCGCCAACTTACAAGGCCTTTCTGTGTAAACAATACC8020.21393113124754926No Hit
AGCTGGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATACTCTATAA7570.2019275141575995No Hit
ATATAAGAGAGAAACTACACATAGCGCCTCATTTTGTGGGTCACCATATT7420.19792630846094958No Hit
GTAAAAAGGGACTCAAGATGCTGTACAGACTTGGCCCCCAATACCACATC7300.1947253439036296No Hit
GTAGTTCCCCCTAGAAAATTGAGAGAAGTCCACCACGAGTCTAGACTCTG7270.19392510276429964No Hit
TCCATAGAGTGTGTAAATAGTGTCTGGTTTGGAAGTAATGATTAACTAGA7000.1867229325103298No Hit
GTTATAGAGTATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCAGC6890.18378871499945315No Hit
GTATAAAGGGACTCAAGATGCTGTACAGACTTGGCCCCCAATACCACATC6580.17551955655971No Hit
GATTGAGATCTTCTGCGACGCGGCGATTGAGACCTTCGTCTGCGAGGCGA6580.17551955655971No Hit
CATTTACACCAAGACATCATCAAAAAATGTGAACAGTTTGTAGGCCCACT6510.1736523272346067No Hit
CTTTAGGACATGGACATTGACCCTTATAAAGAATTTGGAGCTTCCGTGGA6460.17231859200239005No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCAGAGAGTCCAA6450.17205184495594672No Hit
GAACAAATGGCACTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCAC6430.17151835086306005No Hit
AAACCAGACACTATTTACACACTCTATGGAAGGCGGGTATATTATATAAG6370.16991786858440008No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG6310.1683173863057401No Hit
CAGTAAACCCTGTTCCGACTACTGCCTCTCCCTTATCGTCAATCTTCTCG6290.16778389221285347No Hit
GGGTCTCACCCCACCGCACGGAGGCCTTTTGGGGTGGAGCCCTCAGGCTC6250.16671690402708017No Hit
CCCTTATCCAATGGTAAATATTTGGTAACCTTTGGATAAAACCTAGCAGG6120.1632491924233169No Hit
CCTCTTGTCCTCCAACTTGTCCTGGTTATCGCTGGATGTGTCTGCGGCGT6040.16111521605177026No Hit
ACATAGAGGTTCCTTGAGCAGTAGTCATGCAGGTGCGGCATGGTCCCGTG6040.16111521605177026No Hit
GATCCAGCATCTAGAGACCTAGTAGTCAGTTATGTCAACAATAATGTGGG5900.15738075740156365No Hit
AGATTCGTTAGATCTCCCCAGCAAAGAATTGCTTGCCTGAGTGCAGTATG5890.15711401035512035No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC5860.15631376921579035No Hit
GCCTGAGGATGAGTGTTTCTCAAAGGTGGAGACAGCGGGGTAGGCTGCCT5700.1520458164726971No Hit
GTGTAAATAGTGTCTAGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT5660.1509788282869238No Hit
GGATAGGGGCATTTGGTGGTCTATAAGCTGGAGGAGTGCGAATCCACACT5560.14831135782249052No Hit
CTTTAGGACATGGACATTGACCCTTATAAAGAATTTGGAGCTACCGTGGA5560.14831135782249052No Hit
CTCTATAACTGTTTCTCTTCCAAAGGTGAGACAAGAAATGTGAAACCACA5550.1480446107760472No Hit
GTTTGGTGGAAGGTTGTGGAGTTCCACTGCATGGCCTGAGGATGAGTGTT5540.14777786372960386No Hit
GGGCACCACCGTGTGTCTTGGCCAAAATTCGCAGTCCCCAACCTCCAATC5500.14671087554383053No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA5500.14671087554383053No Hit
GTGTAAATGTATATTAGGAAAAGATGGTGTTTTCCAATGAGGATTAAAGA5500.14671087554383053No Hit
GTAGAAGAATAAAGCCCAGTAAAGTTCCCCACCTTATGAGTCCAAGGAAT5470.14591063440450056No Hit
GTGTTCTTGTGGCAAGGACCCATAACATCCAATGACATAACCCATGAAAT5450.1453771403116139No Hit
CTTTATACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAA5170.1379082230112007No Hit
CTTTTTACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAA5110.13630774073254073No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTCCGGGAGAGGACAACAGAGT5100.1360409936860974No Hit
GTGTGAAAGGGGCAGCAAAACCTAAAAGACCCACAATTCGTTGACATACT5090.1357742466396541No Hit
GGGGAACTACCGTGTGTCTTGGCCAAAATTCGCAGTCCCCAACCTCCAAT5010.13364027026810746No Hit
GTTTAATACCCTTATCCAATGGTAAATATTTGGTAACCTTTGGATAAAAC4970.13257328208233415No Hit
GGATAAGGGTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACT4970.13257328208233415No Hit
CATTTAAACCCTAACAAAACAAAGAGATGGGGTTACTCTCTAAATTTCAT4950.1320397879894475No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGAAGAGGA4940.13177304094300415No Hit
GCCTACCCCGCTGTCTCCACCTTTGAGAAACACTCATCCTCAGGCCATGC4940.13177304094300415No Hit
ATATATCCCTGCTGGTGGCTCCAGTTCAGGAACAGTAAACCCTGTTCCGA4940.13177304094300415No Hit
TTATATAAGAGAGAAACTACACATAGCGCCTCATTTTGTGGGTCACCATA4940.13177304094300415No Hit
GTGTAAACAATACCTGAACCTTTACCCCGTTGCCCGGCAACGGCCAGGTC4890.13043930571078752No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCTGCAATGTCAACGACCGACCT4870.12990581161790085No Hit
GTCTCCTGAGCATTGTTCACCTCACCATACTGCACTCAGGCAAGCAATTC4860.12963906457145752No Hit
GGTATAAAGGGACTCAAGATGCTGTACAGACTTGGCCCCCAATACCACAT4850.12937231752501419No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG4850.12937231752501419No Hit
ACCCAGCCCGAATGCTCCAGCTCCTACCTTGTTGGCGTCTGGCCAGGTGT4820.12857207638568421No Hit
ACACATAGCGCCTCATTTTGTGGGTCACCATATTCTTGGGAACAAGATCT4790.12777183524635422No Hit
CCTCTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGC4750.12670484706058094No Hit
GTACCAAACCTTCGGACGGAAATTGCACCTGTATTCCCATCCCATCATCC4740.1264381000141376No Hit
GTTTGTAGGCCCACTCACAGTTAATGAGAAAAGAAGGTTGCAATTGATTA4740.1264381000141376No Hit
GAATCTTTCCACCAGCAATCCTCTGGGATTCTTTCCCGACCACCAGTTGG4710.1256378588748076No Hit
CCAATTGGATCCAGCCTTCAGAGCAAACACCGCAAATCCAGATTGGGACT4690.12510436478192097No Hit
CTCTCGTCCCCTTCTCCGTCTGCCGTTCCGACCGACCACGGGGCGCACCT4630.12350388250326098No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA4560.12163665317815768No Hit
GATACATAGAGGTTCCTTGAGCAGTAGTCATGCAGGTGCGGCATGGTCCC4520.12056966499238438No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTTTGT4520.12056966499238438No Hit
ATCTAAATCTCGGGAATCTCAATGTTAGTATTCCTTGGACTCATAAGGTG4430.11816894157439442No Hit
GGTCCCCTAGAAGAAGAACTCCCTCGCCTCGCAGACGAAGGTCTCAATCG4360.11630171224929112No Hit
CTCTGGGATCTTGCAGAGTTTGGTGGAAGGTTGTGGAGTTCCACTGCATG4360.11630171224929112No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA4360.11630171224929112No Hit
GTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACT4320.1152347240635178No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT4280.11416773587774448No Hit
GGTAAAAAGGGACTCAAGATGCTGTACAGACTTGGCCCCCAATACCACAT4280.11416773587774448No Hit
GTACAGTAGAAGAATAAAGCCCAGTAAAGTTCCCCACCTTATGAGTCCAA4240.11310074769197119No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTAGGACATGGACATTGA4220.11256725359908454No Hit
GTATGTCAACGAATTGTGGGTCTTTTAGGTTTTGCTGCCCCTTTCACACA4160.11096677132042457No Hit
GGATAACCACATTGTGTGAAAGGGGCAGCAAAACCTAAAAGACCCACAAT4160.11096677132042457No Hit
GGGTTTACTGTTCCTGAACTGGAGCCACCAGCAGGGAGATATAGGCCTCT4080.10883279494887793No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT4070.1085660479024346No Hit
AAACTACACATAGCGCCTCATTTTGTGGGTCACCATATTCTTGGGAACAA4060.10829930085599128No Hit
GCACGGGACCATGCCGCACCTGCATGACTACTGCTCAAGGAACCTCTATG4030.1074990597166613No Hit
GGGGCACCACCGTGTGTCTTGGCCAAAATTCGCAGTCCCCAACCTCCAAT4010.10696556562377463No Hit
ATTTCCGTCCGAAGGTTTGGTACAGCAACAGGAGGGATACATAGAGGTTC3930.104831589252228No Hit
GTGTTGATAGGATAGGGGCATTTGGTGGTCTATAAGCTGGAGGAGTGCGA3920.10456484220578467No Hit
ACTACACATAGCGCCTCATTTTGTGGGTCACCATATTCTTGGGAACAAGA3910.10429809515934134No Hit
GTGTAGTTTCTCTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAA3900.10403134811289802No Hit
GAATACAGGTGCAATTTCCGTCCGAAGGTTTGGTACAGCAACAGGAGGGA3890.10376460106645469No Hit
GTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTAG3840.10243086583423805No Hit
GTCTTGGTGTAAATGTATATTAGGAAAAGATGGTGTTTTCCAATGAGGAT3840.10243086583423805No Hit
CGGTGGTGCCCCCTAGAAAATTGAGAGAAGTCCACCACGAGTCTAGACTC3830.10216411878779472No Hit
GTAGTCATGCAGGTGCGGCATGGTCCCGTGCTGGTGGTTGAGGATCCTGG3820.10189737174135138No Hit
GGTGAAACCCAGCCCGAATGCTCCAGCTCCTACCTTGTTGGCGTCTGGCC3780.10083038355557807No Hit
CCTTTATACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA3780.10083038355557807No Hit
GGTCTGGAGCAAGCATTATCGGGACTGATAACTCTGTTGTCCTCTCCCGG3780.10083038355557807No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)