FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488361

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488361
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences553001
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN33390.603796376498415No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC21110.3817352952345475No Hit
TCCATAGAGTGTGTAAATAGTGTCTAGTTTGGAAGTAATGATTAACTAGA18130.3278475084131855No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACATAGCGCC15700.2839054540588534No Hit
GTTCAGTGCAGGGTCCCCAATCCTCGAGAAGATTGACGATAAGGGAGAGG15380.27811884607803605No Hit
GTTTACGCAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGCTTA14390.26021652763738223No Hit
CCTTATGAGTCCAAGGAATACTAACATTGGGATCCCCGAGATTGAGATCT13890.25117495266735507No Hit
CTTCCGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTTTTTCCTTCAGTA13150.2377934217117148No Hit
CCTTATGAGTCCAAGGAATATTAACATTGAGATCCCCGAGATTGAGATCT12060.21808278827705557No Hit
CCCTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGAT11540.20867955030822732No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACCACCGTG11360.2054245833190175No Hit
GTATAATGTGTTCTTGTGGCAAGGACCCATGACATCCAATGACATAACCC10520.19023473736937185No Hit
GCACCGAACATGGAGAACATCACATCAGGATTCCTAGGACCCCTTCTCGT9960.1801081734029414No Hit
GGCCCTGAAGTGGAACTCCACAACCTTCCACCAAACTCTGCAAGATCCCC9820.17757653241133378No Hit
CTCACCAGGTGGGATCCTTTACTGGGCTTTATTCTTCTACTGTACCTGTC9520.1721515874293175No Hit
GTTATAGAGTATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCAGC9360.1692582834389088No Hit
GAATACTAACATTGGGATCCCCGAGATTGAGATCTTCTGCGACGCGGCGA9350.16907745193950824No Hit
CCTGGTGAGTCCAAGGAATATTAACATTGAGATCCCCGAGATTGAGATCT9090.16437583295509411No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTATACTGCACCTGTC9020.16311001245929033No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTCATTGGAAAACACCATCTTTTC8640.15623841548206965No Hit
CCCTTGTAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGAT8620.15587675248326857No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC8570.15497259498626584No Hit
CTGTATAAACAATATCTGAACCTTTACCCCGTTGCCCGGCAACGGCCAGG8190.1481009980090452No Hit
CCTCCAGCTTATAGACCATCAAATGCCCCTATCCTATCAACACTTCCGGA8100.1464735145144403No Hit
CCTGGTGAGTCCAAGGAATACTAACATTGGGATCCCCGAGATTGAGATCT7990.14448436802103432No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC7740.13996358053602073No Hit
CTACAAGGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCCTCAACCA7630.13797443404261475No Hit
ATATAAGAGAGAAACAACACATAGCGCCTCATTTTGTGGGTCACCATATT7600.13743193954441313No Hit
GTGTAAATAGTGTCTAGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT7330.13254948906059844No Hit
CTCTATAACTGTGTCTCTTCCAAAGGTGAGACAAGAAATGTGAAACCACA7320.1323686575611979No Hit
GCCTAATCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC7290.13182616306299627No Hit
CTATCAGGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCCTCAACCA7270.1314645000641952No Hit
GTTTGTAGTATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGGCCTCCGTG7220.13056034256719246No Hit
ACCCTTCTCGGGGTCGCTTGGGACTCTCTCGTCCCCTTCTCCGTCTGCCG7200.13019867956839137No Hit
CTTCAGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTTTTTCCTTCAGTA7100.12839036457438596No Hit
CCCTCACAAAACAAAGAGATGGGGTTACTCTCTAAATTTCATGGGTTATG7080.12802870157558485No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACCTTCCACCAAACTCTGCAAG7060.12766703857678377No Hit
TTAAAGCCCTTGTATGCTTGTATTCAATCTAAGCAGGCTTTCACTTTCTC6950.1256778920833778No Hit
GGATAAGGGTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACT6710.12133793609776473No Hit
CCTCACCATACTGCACTCAGGCAAGCAATTCTTTGCTGGGGGGAACTAAT6700.12115710459836421No Hit
CCCTTATCCAATGGTAAATATTTGGTAACCTTTGGATAAAACCTAGCAGG6610.1195296211037593No Hit
TTCTTATACTGCACCTGTCTTTAATCCTCATTGGAAAACACCATCTTTTC6520.11790213760915441No Hit
GTATAAACAATATCTGAACCTTTACCCCGTTGCCCGGCAACGGCCAGGTC6490.11735964311095279No Hit
GCACTGAACATGGAGAACATCACATCAGGATTCCTAGGACCCCTTCTCGT6470.1169979801121517No Hit
GGCCATGCAGTGGAACTCCACAACCTTCCACCAAACTCTGCAAGATCCCC6270.11338135012414081No Hit
GTATAAGAATAAAGCCCAGTAAAGTTTCCCACCTTATGAGTCCAAGGAAT6240.1128388556259392No Hit
CACTTCTTGATAATGTCTTGGTGTAAATGTATATTAGGAAAAGATGGTGT6240.1128388556259392No Hit
TTATATAAGAGAGAAACAACACATAGCGCCTCATTTTGTGGGTCACCATA6220.11247719262713811No Hit
CTATTTACACACTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAA6220.11247719262713811No Hit
CATCATACAAAAAATCAAAGAATGTTTTCGTAAACTTCCTGTTAACAGGC6130.11084970913253323No Hit
GTGTAAATGTATATTAGGAAAAGATGGTGTTTTCCAATGAGGATTAAAGA5960.10777557364272398No Hit
CCCCTATCCTATCAACACTTCCGGAGACTTGTGTTGTTAGACGACGAGGC5860.10596725864871855No Hit
GTTTATACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGCTTA5830.1054247641505169No Hit
CATCCAGCGATAACCAGGACAAGTTGGAGGACAGGAGGTTGGTGAGTGAT5810.10506310115171581No Hit
CCACAAGTCTAGATTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAA5770.10433977515411366No Hit
GTGGTTCCCCCTAGAAAATTGAGAGAAGTCCACCACGAGTCTAGACTCTG5740.10379728065591201No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCAGAGAGTCCAA5700.10307395465830983No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAGTGTCTAGT5680.10271229165950875No Hit
CACTTTTTGATAATGTCTTGGTGTAAATGTATATTAGGAAAAGATGGTGT5560.10054231366670224No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)