FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002488362

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488362
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences368680
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC18640.5055875013561897No Hit
TCCATAGAGTGTGTAAATAGTGTCTAGTTTGGAAGTAATGATTAACTAGA16870.4575783877617446No Hit
CTCTATGGAAGGCGGGTATCTTATATAAGAGAGAAACAACACATAGCGCC15380.41716393620483894No Hit
TTGTACACCTCACCATACTGCACTCAGGCAAGCAATTCTTTGCTGGGGGG13230.35884778127373335No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG11610.3149072366279701No Hit
CTATTTACACACTCTATGGAAGGCGGGTATCTTATATAAGAGAGAAACAA11020.29890419876315505No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC10270.27856135401974613No Hit
CTCTAGAACTGTATCTCTTCCAAAGGTGAGACAAGAAATGTGAAACCACA10080.2734078333514159No Hit
ATGTAAGACCTTGGGCAAGCTTCGATGGGCGTTCACGGTGGTCTCCATGC9700.26310079201475534No Hit
GTTCTAGAGTATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCTCC9390.2546924161874796No Hit
GTATAAACAATACCTGAACCTTTACCCCGTTGCCCGGCAACGGCCAGGTC9220.2500813713789736No Hit
GTGTAAATAGTGTCTAGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT8650.23462080937398286No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCAGAGAGTCCAA8430.22865357491591623No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACCTTCCACCAAACTCTGCAAG8320.22566995768688292No Hit
CTAATCTCCTCCCCCAACTCCTCCCAGTCTTTGAACAAACAGTCTTTGAA8110.21997396115872844No Hit
ATTTTGTGGGTCACCATATTCTTGGGAACAAGATCTACAGCATGGGGCAG7600.20614082673321038No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA7440.20180101985461646No Hit
CCCTTATCCAATGGTAAATATTTGGTAACCTTTGGATAAAACCTAGCAGG7230.19610502332646196No Hit
GTGTAAATGTATATTAGGAAAGGATGGAGTTTTCCAATGAGGATTAAAGA7220.19583378539654986No Hit
CTACTGTACCTGTCTTTAATCCTCATTGGAAAACTCCATCCTTTCCTAAT7200.1952913095367256No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGCTTCGATGGGCGTTC7190.1950200716068135No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN7100.19257893023760445No Hit
GGATAAGGGTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACT7040.1909515026581317No Hit
CATTTAAACCCTAACAAAACAAAGAGATGGGGTTACTCTCTAAATTTCAT6960.18878159921883478No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC6880.18661169577953782No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAGTTTGTAGGCCCACT6720.1822718889009439No Hit
ACATAGAGGTTCCTTGAGCAGTAGTCATGCAGGTGCGGCATGGTCCCGTG6600.17901703374199848No Hit
TGTTTATACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGCTT6210.16843875447542586No Hit
CCTTATGAGTCCAAGGAATACTAACATTGAGATTCCCGAGATTGAGATCT6060.16437018552674407No Hit
ACCGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCAGTACG6000.16274275794727133No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCCTCAACCACC5760.1562330476293805No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG5700.15460562004990777No Hit
GGGTAAAGGTTCAGGTATTGTTTATACAGAAAGGCCTTGTAAGTTGGCGA5700.15460562004990777No Hit
GTTTAATACCCTTATCCAATGGTAAATATTTGGTAACCTTTGGATAAAAC5550.15053705110122598No Hit
ATATAGGCCTCTCACTCTGGGATCTTGCAGAGTTTGGTGGAAGGTTGTGG5480.14863838559184117No Hit
GCCTGAGGATGAGTGTTTCTCAAAGGTGGAGACAGCGGGGTAGGCTGCCT5390.14619724422263208No Hit
GTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACT5380.14592600629271996No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT5340.1448410545730715No Hit
GTATGGATCGGCAGAGGATCCAGAAAGGTTCCACGCATGCGCTGATGGCC5300.14375610285342302No Hit
CCTTTACCCCGTTGCCCGGCAACGGCCAGGTCTATGCCAAGTGTTTGCTG5250.14239991320386242No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGAAGAGGA5230.1418574373440382No Hit
CCTCCTCCTTATAGACCACCAAATGCCCCTATCCTATCAACACTTCCGGA5150.13968753390474126No Hit
GGCTTTCACTTTCTCGCCAACTTACAAGGCCTTTCTGTATAAACAATACC5150.13968753390474126No Hit
TTGTTCAGCTCACCATACTGCACTCAGGCAAGCAATTCTTTGCTGGGGGG5140.13941629597482913No Hit
GCCTAATCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC5050.13697515460562004No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT5030.13643267874579582No Hit
GTGTGAAAGGGGCAGCAAAACCTAAAAGACCCACAATTCGTTGACATACT5010.1358902028859716No Hit
GGGTTTACTGTTCCTGAACTGGAGCCACCAGCAGGGAAATATAGGCCTCT4980.13507648909623524No Hit
CCTCTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGC4960.13453401323641098No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC4960.13453401323641098No Hit
GGATAGGGGCATTTGGTGGTCTATAAGGAGGAGGAGTGCGAATCCACACT4910.13317782358685037No Hit
GGTCTATGCCAAGTGTTTGCTGACGCAACCCCCACTGGCTGGGGCTTGGT4860.1318216339372898No Hit
TCACCATACTGCACTCAGGCAAGCAATTCTTTGCTGGGGGGAATTAATGA4740.12856677877834435No Hit
GCTGAACAATGCTCAGGAGACTCTAAGGCTTGCCGATACAGAGCTGAGGC4730.12829554084843225No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA4700.1274818270586959No Hit
CCTTATAGACCACCAAATGCCCCTATCCTATCAACACTTCCGGAGACTAC4620.12531192361939894No Hit
CTACAAACTTTGCCAGCAAATCCACCTCCTGCCTCCACCAATCGCCAGTC4600.1247694477595747No Hit
CAGTAAACCCTGTTCCGACTACTGCCTCTCCCTTATCGTCAATCTTCTCG4560.12368449603992622No Hit
GGCCTATTGATTGGAAAGTATGTCAACGAATTGTGGGTCTTTTAGGTTTT4550.1234132581100141No Hit
GAATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGA4530.12287078225018987No Hit
GTTTGTAGGCCCACTCACAGTTAATGAGAAAAGAAGGTTGCAATTGATTA4400.11934468916133233No Hit
CCTGTATTCCCATCCCATCATCCTGGGCTTTCGGAAAATTCCTATGGGAG4380.11880221330150809No Hit
ATATAAGAGAGAAACAACACATAGCGCCTCATTTTGTGGGTCACCATATT4360.11825973744168386No Hit
ATGTATATTTGCGGGAGAGGACAACAGAGTTATCAGTCCCGATAATGCTT4350.11798849951177173No Hit
GAACATGGAGAACATCACATCAGGATTCCTAGGACCCCTTCTCGTGTTAC4340.11771726158185959No Hit
GATACATAGAGGTTCCTTGAGCAGTAGTCATGCAGGTGCGGCATGGTCCC4310.11690354779212324No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAGTCCCTTTTTACC4230.1147336443528263No Hit
ATATTTCCCTGCTGGTGGCTCCAGTTCAGGAACAGTAAACCCTGTTCCGA4210.11419116849300207No Hit
CTCTTATATAAGATACCCGCCTTCCATAGAGTGTGTAAATAGTGTCTAGT4190.11364869263317781No Hit
GACGGGGAGTCCGCGTAAAGAGAGGTGCGCCCCGTGGTCGGTCGGAACGG4190.11364869263317781No Hit
TCCTTATAGACCACCAAATGCCCCTATCCTATCAACACTTCCGGAGACTA4180.1133774547032657No Hit
GGTAAATATTTGGTAACCTTTGGATAAAACCTAGCAGGCATAATCAATTG4170.11310621677335359No Hit
CCCCTATCCTATCAACACTTCCGGAGACTACTGTTGTTAGACGACGAGGC4150.11256374091352934No Hit
CTCTGGGATCTTGCAGAGTTTGGTGGAAGGTTGTGGAGTTCCACTGCATG4140.11229250298361723No Hit
GCTAGATACCGCCTCAGCTCTGTATCGGCAAGCCTTAGAGTCTCCTGAGC4130.1120212650537051No Hit
ATACTGCACTCAGGCAAGCAATTCTTTGCTGGGGGGAATTAATGACTCTA4110.11147878919388088No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAG4100.11120755126396875No Hit
GTCTTGGTGTAAATGTATATTAGGAAAGGATGGAGTTTTCCAATGAGGAT4060.11012259954432028No Hit
GAATACAGGTGCAATTTCCGTCCGAAGGTTTGGTACAGCAACAGGAGGGA4050.10985136161440817No Hit
GATCTACAGCATGGGGCAGAATCTTTCCACCAGCAATCCTCTGGGATTCT3980.10795269610502332No Hit
GTCCCGTGCTGGTGGTTGAGGATCCTGGAATTAGAGGACAAACGGGCAAC3970.10768145817511121No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGAGAGGACAACAGAGT3960.10741022024519908No Hit
CTATAAGGAGGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATACTC3950.10713898231528697No Hit
ATATGAGAGTTGACATAACTGACTACTAGGTCTCTAGATGTTGGATCTTC3950.10713898231528697No Hit
CCCTTATCGTCAATCTTCTCGAGGATTGGGGACCCTGCACTGAACATGGA3930.10659650645546273No Hit
GCACGGGACCATGCCGCACCTGCATGACTACTGCTCAAGGAACCTCTATG3930.10659650645546273No Hit
GGATAATAAGGTTTAATACCCTTATCCAATGGTAAATATTTGGTAACCTT3910.1060540305956385No Hit
CTCCAGACCTGCTGCGAGCAAAACAAGCGGCTAGGAGTTCCGCAGTATGG3910.1060540305956385No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA3900.10578279266572638No Hit
CACCAAGACATTATCAAAAAATGTGAACAGTTTGTAGGCCCACTCACAGT3880.10524031680590212No Hit
CTTCTGGACTATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATC3840.10415536508625366No Hit
GTACAGTAGAAGAATAAAGCCCGGTAAAGTTCCCCACCTTATGAGTCCAA3830.10388412715634154No Hit
GTCCAAGGAATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGAC3830.10388412715634154No Hit
ATGTGGTTATCCTGCTTTAATGCCCTTGTATGCTTGTATTCAATCTAAGC3820.10361288922642942No Hit
GCTTTAATGCCCTTGTATGCTTGTATTCAATCTAAGCAGGCTTTCACTTT3810.1033416512965173No Hit
TAACCACATTGTGTGAAAGGGGCAGCAAAACCTAAAAGACCCACAATTCG3810.1033416512965173No Hit
ATTTACACACTCTATGGAAGGCGGGTATCTTATATAAGAGAGAAACAACA3790.10279917543669306No Hit
GACATAACCCATGAAATTTAGAGAGTAACCCCATCTCTTTGTTTTGTTAG3790.10279917543669306No Hit
GGATAACCACATTGTGTGAAAGGGGCAGCAAAACCTAAAAGACCCACAAT3770.10225669957686884No Hit
GTTTATACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGCTTA3750.1017142237170446No Hit
GAATACAAGCATACAAGGGCATTAAAGCAGGATAACCACATTGTGTGAAA3730.10117174785722034No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTG3720.10090050992730823No Hit
GCCTTAGAGTCTCCTGAGCATTGTACACCTCACCATACTGCACTCAGGCA3710.1006292719973961No Hit
CTCATAAGGTGGGGAACTTTACCGGGCTTTATTCTTCTACTGTACCTGTC3700.10035803406748399No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)