FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002488363

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488363
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences368680
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATAGAGTGTGTAAATAGTGTCTAGTTTGGAAGTAATGATTAACTAGA16050.4353368775089509No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC15580.42258869480308126No Hit
CTCTATGGAAGGCGGGTATCTTATATAAGAGAGAAACAACACATAGCGCC14130.383259194965824No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG13530.3669849191710969No Hit
TTGTACACCTCACCATACTGCACTCAGGCAAGCAATTCTTTGCTGGGGGG11810.3203319952262124No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC9970.27042421612238254No Hit
CTCTAGAACTGTATCTCTTCCAAAGGTGAGACAAGAAATGTGAAACCACA9440.25604860583704026No Hit
CTATTTACACACTCTATGGAAGGCGGGTATCTTATATAAGAGAGAAACAA9400.2549636541173918No Hit
GTATAAACAATACCTGAACCTTTACCCCGTTGCCCGGCAACGGCCAGGTC9240.2506238472387979No Hit
GTTCTAGAGTATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCTCC9240.2506238472387979No Hit
GTGTAAATAGTGTCTAGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT8570.23245090593468593No Hit
ATGTAAGACCTTGGGCAAGCTTCGATGGGCGTTCACGGTGGTCTCCATGC8130.22051643701855267No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACCTTCCACCAAACTCTGCAAG7990.21671910599978303No Hit
CCCTTATCCAATGGTAAATATTTGGTAACCTTTGGATAAAACCTAGCAGG7940.21536291635022242No Hit
CTACTGTACCTGTCTTTAATCCTCATTGGAAAACTCCATCCTTTCCTAAT7570.205327112943474No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN7060.19149397851795594No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCAGAGAGTCCAA6940.18823912335901052No Hit
GGATAAGGGTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACT6840.18552674405988934No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA6810.18471303027015298No Hit
ATTTTGTGGGTCACCATATTCTTGGGAACAAGATCTACAGCATGGGGCAG6710.1820006509710318No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAGTTTGTAGGCCCACT6690.18145817511120754No Hit
CATTTAAACCCTAACAAAACAAAGAGATGGGGTTACTCTCTAAATTTCAT6530.17711836823261365No Hit
ACCGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCAGTACG6320.17142237170445915No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC6200.16816751654551373No Hit
GTGTAAATGTATATTAGGAAAGGATGGAGTTTTCCAATGAGGATTAAAGA6090.16518389931648042No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGCTTCGATGGGCGTTC5980.1622002820874471No Hit
GCCTGAGGATGAGTGTTTCTCAAAGGTGGAGACAGCGGGGTAGGCTGCCT5940.16111533036779863No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCCTCAACCACC5930.16084409243788653No Hit
CCTTATGAGTCCAAGGAATACTAACATTGAGATTCCCGAGATTGAGATCT5700.15460562004990777No Hit
GTTTAATACCCTTATCCAATGGTAAATATTTGGTAACCTTTGGATAAAAC5670.15379190626017142No Hit
GCCTAATCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC5560.1508082890311381No Hit
CCTTTACCCCGTTGCCCGGCAACGGCCAGGTCTATGCCAAGTGTTTGCTG5390.14619724422263208No Hit
CCTCCTCCTTATAGACCACCAAATGCCCCTATCCTATCAACACTTCCGGA5330.14456981664315938No Hit
GTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACT5290.14348486492351092No Hit
GGGTTTACTGTTCCTGAACTGGAGCCACCAGCAGGGAAATATAGGCCTCT5260.14267115113377457No Hit
ACATAGAGGTTCCTTGAGCAGTAGTCATGCAGGTGCGGCATGGTCCCGTG5250.14239991320386242No Hit
GAATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGA5240.1421286752739503No Hit
CAGTAAACCCTGTTCCGACTACTGCCTCTCCCTTATCGTCAATCTTCTCG5210.14131496148421394No Hit
GTATGGATCGGCAGAGGATCCAGAAAGGTTCCACGCATGCGCTGATGGCC5170.14023000976456548No Hit
ATATAGGCCTCTCACTCTGGGATCTTGCAGAGTTTGGTGGAAGGTTGTGG5040.13670391667570794No Hit
GGCTTTCACTTTCTCGCCAACTTACAAGGCCTTTCTGTATAAACAATACC5040.13670391667570794No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG5010.1358902028859716No Hit
ATATTTCCCTGCTGGTGGCTCCAGTTCAGGAACAGTAAACCCTGTTCCGA4900.13290658565693828No Hit
GGATAGGGGCATTTGGTGGTCTATAAGGAGGAGGAGTGCGAATCCACACT4810.13046544428772922No Hit
TTGTTCAGCTCACCATACTGCACTCAGGCAAGCAATTCTTTGCTGGGGGG4780.12965173049799283No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT4780.12965173049799283No Hit
CCTCTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGC4740.12856677877834435No Hit
GTGTGAAAGGGGCAGCAAAACCTAAAAGACCCACAATTCGTTGACATACT4700.1274818270586959No Hit
CTCTGGGATCTTGCAGAGTTTGGTGGAAGGTTGTGGAGTTCCACTGCATG4650.1261256374091353No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA4610.1250406856894868No Hit
TGTTTATACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGCTT4600.1247694477595747No Hit
CTAATCTCCTCCCCCAACTCCTCCCAGTCTTTGAACAAACAGTCTTTGAA4580.12422697189975046No Hit
CCCCTATCCTATCAACACTTCCGGAGACTACTGTTGTTAGACGACGAGGC4560.12368449603992622No Hit
GGGTAAAGGTTCAGGTATTGTTTATACAGAAAGGCCTTGTAAGTTGGCGA4560.12368449603992622No Hit
GGTCTATGCCAAGTGTTTGCTGACGCAACCCCCACTGGCTGGGGCTTGGT4540.123142020180102No Hit
GAACATGGAGAACATCACATCAGGATTCCTAGGACCCCTTCTCGTGTTAC4490.12178583053054139No Hit
CTACAAACTTTGCCAGCAAATCCACCTCCTGCCTCCACCAATCGCCAGTC4420.11988716502115655No Hit
CCTTATAGACCACCAAATGCCCCTATCCTATCAACACTTCCGGAGACTAC4360.11825973744168386No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGAAGAGGA4340.11771726158185959No Hit
CTCCAGACCTGCTGCGAGCAAAACAAGCGGCTAGGAGTTCCGCAGTATGG4300.11663230986221113No Hit
GGCCTATTGATTGGAAAGTATGTCAACGAATTGTGGGTCTTTTAGGTTTT4230.1147336443528263No Hit
ATATAAGAGAGAAACAACACATAGCGCCTCATTTTGTGGGTCACCATATT4180.1133774547032657No Hit
CTTCTGGACTATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATC4160.11283497884344146No Hit
GTTTGTAGGCCCACTCACAGTTAATGAGAAAAGAAGGTTGCAATTGATTA4130.1120212650537051No Hit
AATATACATCGTTTCCATGGCTGCTAGGCTGTACTGCCAACTGGATCCTG4120.11175002712379299No Hit
CTCTCATATGGGCCTAAAGTTTAGGCAACTCTTGTGGTTTCACATTTCTT4070.1103938374742324No Hit
GGATAACCACATTGTGTGAAAGGGGCAGCAAAACCTAAAAGACCCACAAT4060.11012259954432028No Hit
CCTGTATTCCCATCCCATCATCCTGGGCTTTCGGAAAATTCCTATGGGAG4060.11012259954432028No Hit
CTATAAGGAGGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATACTC4050.10985136161440817No Hit
GGTAAATATTTGGTAACCTTTGGATAAAACCTAGCAGGCATAATCAATTG4050.10985136161440817No Hit
GCTAGATACCGCCTCAGCTCTGTATCGGCAAGCCTTAGAGTCTCCTGAGC4010.10876640989475968No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC4010.10876640989475968No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGAGAGGACAACAGAGT4000.10849517196484756No Hit
GTCTTGGTGTAAATGTATATTAGGAAAGGATGGAGTTTTCCAATGAGGAT4000.10849517196484756No Hit
CCCTTATCGTCAATCTTCTCGAGGATTGGGGACCCTGCACTGAACATGGA3990.10822393403493544No Hit
CTCTTATATAAGATACCCGCCTTCCATAGAGTGTGTAAATAGTGTCTAGT3990.10822393403493544No Hit
ATACTGCACTCAGGCAAGCAATTCTTTGCTGGGGGGAATTAATGACTCTA3980.10795269610502332No Hit
ATATGAGAGTTGACATAACTGACTACTAGGTCTCTAGATGTTGGATCTTC3970.10768145817511121No Hit
ATGTATATTTGCGGGAGAGGACAACAGAGTTATCAGTCCCGATAATGCTT3950.10713898231528697No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT3930.10659650645546273No Hit
TCACCATACTGCACTCAGGCAAGCAATTCTTTGCTGGGGGGAATTAATGA3920.10632526852555062No Hit
CCCCAAAAGGCCTCCGTGCGGTGGGGTGAAACCCAGCCCGAATGCTCCAG3910.1060540305956385No Hit
GCTTTAATGCCCTTGTATGCTTGTATTCAATCTAAGCAGGCTTTCACTTT3910.1060540305956385No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAGTCCCTTTTTACC3880.10524031680590212No Hit
ATCCCAGAGTGAGAGGCCTATATTTCCCTGCTGGTGGCTCCAGTTCAGGA3870.10496907887599001No Hit
TCCTTATAGACCACCAAATGCCCCTATCCTATCAACACTTCCGGAGACTA3810.1033416512965173No Hit
GTACAGTAGAAGAATAAAGCCCGGTAAAGTTCCCCACCTTATGAGTCCAA3810.1033416512965173No Hit
GCACGGGACCATGCCGCACCTGCATGACTACTGCTCAAGGAACCTCTATG3810.1033416512965173No Hit
GTCCCGTGCTGGTGGTTGAGGATCCTGGAATTAGAGGACAAACGGGCAAC3800.10307041336660519No Hit
GTACCAAACCTTCGGACGGAAATTGCACCTGTATTCCCATCCCATCATCC3780.10252793750678095No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCTGCAATGTCAACGACCGACCT3730.10117174785722034No Hit
GGTGGAGACAGCGGGGTAGGCTGCCTTCCTGACTGGCGATTGGTGGAGGC3720.10090050992730823No Hit
GTAGAAGAATAAAGCCCGGTAAAGTTCCCCACCTTATGAGTCCAAGGAAT3710.1006292719973961No Hit
ATTTACACACTCTATGGAAGGCGGGTATCTTATATAAGAGAGAAACAACA3710.1006292719973961No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT3710.1006292719973961No Hit
GTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTAG3690.10008679613757188No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)