FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002488365

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488365
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences438813
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN17270.3935617221914574No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC17070.3890039720792228No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA16570.3776095967986363No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG13770.31380109522735194No Hit
GTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTGCATGTTCAGG12500.28485938201466227No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT10520.2397376559035398No Hit
CAACTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGAAGAGAAACTGT9900.2256086305556125No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC9870.22492496803877735No Hit
GTATACACGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA9770.22264609298266003No Hit
CTATATAAGAGAGAAACTACACGCAGCGCTTCATTTTGTGGGTCACCATA9520.2169489053423668No Hit
GACTTTCTCTTAGAGGTGGAGAGATGGGAGTAGGCTGTCTTCCTGACTGC9510.21672101783675504No Hit
GTCCACAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGAAGAGGA9350.2130748177469674No Hit
CTAAACAGGCTTTCACTTTCTCGCCAACTTACAAGGCCTTTCTGTGTCAA9330.21261904273574395No Hit
CATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA8990.2048708675449451No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG8810.20076889244393398No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT8600.19598325482608767No Hit
GAGACAGTCATCCTCAGGCCATGCAGTGGAACTCCACAACATTCCACCAA8580.19552747981486418No Hit
ATATAAGAGAGAAACTACACGCAGCGCTTCATTTTGTGGGTCACCATATT8560.19507170480364072No Hit
GTACTAAAACTCAAGCAATGTTTTCGGAAACTGCCTGTAAATAGACCTAT8330.18983029217457095No Hit
ATTGACAACAGTGCCAGCAGCCCCTCCTCCTGCCTCCACCAATCGGCAGT8070.18390521702866597No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG7700.17547337932103196No Hit
GTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTAT7510.17114351671440908No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACA7490.17068774170318565No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA7410.1688646416582918No Hit
GTACAAAAACTCAAGCAATGTTTTCGGAAACTGCCTGTAAATAGACCTAT7370.16795309163584488No Hit
CTATTAATAATGTCCTCCTGTAAATGAATGTTAGGAAAGGAGGGAGTTTG7350.16749731662462142No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAACTAATTATGCCTGCTAGG7300.16635787909656277No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG6770.1542798412991411No Hit
CTTATATAAGAGCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCA6640.15131730372618862No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA6320.14402490354661324No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT6240.1422018035017194No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCCTCCTGT6210.14151814098488424No Hit
GTTTTAGTACAATATGTTCCTGTGGTAAAGTACCCCAACTACCAATTACA6040.13764405338948482No Hit
CTTCTGGACTACCAAGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGAAC5960.135820953344591No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG5900.1344536283109206No Hit
GGGTAAAGGTGCAGGTATTGTTGACACAGAAAGGCCTTGTAAGTTGGCGA5890.13422574080530886No Hit
GGAATCGTGCAGGTCTTGCATGGTCCCGTGCTGGTAGTTGATGTTCCTGG5730.1305795407155212No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTGTTTGCTG5710.13012376570429773No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG5480.12488235307522794No Hit
CCCGTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTT5410.12328714053594585No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA5350.12191981550227546No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTG5320.12123615298544027No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC5280.12032460296299335No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTACCAATTACATATCCCATGAA5230.1191851654349347No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCTTAT5220.11895727792932297No Hit
TTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACAC5180.11804572790687605No Hit
CCCTTAACTTCATGGGATATGTAATTGGTAGTTGGGGTACTTTACCACAG5180.11804572790687605No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG5150.11736206539004086No Hit
ATTTAGTAAGGTTAGGATAAAACCTAGCAGGCATAATTAGTTTTAATCTC5150.11736206539004086No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGTTACTCCCTTAACTTCAT5060.11531107783953529No Hit
CCTGCTTAGATTGTATACACGCATATAAAGGCATCAAGGCAGGATAGCCA4970.11326009028902971No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGCAGGGCTTTCCCCCACTGTT4960.11303220278341798No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCCGCTTACAGACCACCAAATGCC4940.11257642777219452No Hit
GACTGTCTCTTAGAGGTGGAGAGATGGGAGTAGGCTGTCTTCCTGACTGC4900.11166487774974762No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACATTCCACCAAGCTCTGCTAG4880.11120910273852416No Hit
CTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCTTG4850.11052544022168898No Hit
ATATTTTCCTGCTGGTGGCTCCAGTTCCGGAACAGTAAACCCTGTTCCGA4850.11052544022168898No Hit
GGTTAGGATAAAACCTAGCAGGCATAATTAGTTTTAATCTCCTTTTTTCA4830.11006966521046549No Hit
GGGTTCACCCCACCACACGGCGGTCTTTTGGGGTGGAGCCCTCAGGCTCA4820.10984177770485377No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTGCGAACCACTGAACA4770.10870234017679513No Hit
TCCCATGGCTGCTCGGGTGTGCTGCCAACTGGATCCTGCGCGGGACGTCC4760.1084744526711834No Hit
GTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCGGGGCCG4710.10733501514312475No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCCGATGGCC4680.10665135262628955No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT4670.10642346512067782No Hit
CCCCCAACTCCTCCCAGTCTTTAAACAAACAGTCTTTGAAGTATGCCTCA4640.10573980260384264No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC4620.10528402759261918No Hit
GTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAAA4540.10346092754772533No Hit
TTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCTTATTATCCTGAA4460.10163782750283151No Hit
GACCCGTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCC4390.1000426149635494No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)