FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002488366

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488366
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences527160
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN26620.5049700280749678No Hit
CTATCAATAATGTCCTCCTGTAAATGAATGTTAGGAAAGGAGGGAGTTTG16510.3131876470141892No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC14710.27904241596479246No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA14020.2659534107291904No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC13920.26405645344866835No Hit
GTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC12780.24243114045071706No Hit
ATTTTGTGGTCTGTAAGCGGGAGGAGTGCGAATCCACACTCCAAAAGACA11460.21739130434782608No Hit
GTTCCGGAACAGTAAACCCTGTTCCGACTACTGCCTCACCCATATCGTCA11350.2153046513392518No Hit
ACGATGCAGGACCCCTAGAAGAAGAACTCCCTCGCCTCGCAGACGAAGGT11320.21473556415509523No Hit
CACCCACGTGTCCTGGCCAAAATTCGCAGTCCCCAACCTCCAATCACTCA11280.2139767812428864No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT10700.20297442901585855No Hit
CAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCTCCGTGAGGTG10410.19747325290234463No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGC10220.19386903406935274No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT10130.1921617725168829No Hit
CTATTAATAATGTCCTCCTGTAAATGAATGTTAGGAAAGGAGGGAGTTTG10100.1915926853327263No Hit
TTTTATGGGTCACCATATTCTTGGGAACAAGAGCTACAGCATGGGAGGTT10060.1908339024205175No Hit
CCACCAAATGCCCCTATCTTATCAACACTTCCGGAAACTACTGTTGTTAG9960.18893694513999545No Hit
GTCCCACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATG9870.1872296835875256No Hit
GTCCTGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC9760.18514303057895135No Hit
GTATGTAAATAATGCCTAGTTTTAAAGTAATGATTAACTGCATGTTCAGG9630.1826769861142727No Hit
TACAAGTGCAGTTTCCGTCCGAAGGTTTTGTACAGCAACAAGAGGGAAAC9570.18153881174595948No Hit
CACTTGAGTGCTCCCCCTAGAAAATTGAGAGAAGTCCACCACGAGTCTAG9260.17565824417634116No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG8700.16503528340541773No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGATGAGAGTCCAA8690.1648455876773655No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACTCAAG8360.15858562865164277No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC8050.15270506108202442No Hit
GCCTCCGCTCTGTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACC8030.15232566962592003No Hit
CTATATAAGAGAGAAACTACACGCAGCGCTTCATTTTGTGGGTCACCATA7940.15061840807345017No Hit
GTTTTAAAGTAATGATTAACTGCATGTTCAGGATAATAAGGTTTAATGCC7710.1462554063282495No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA7630.14473784050383184No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACA7470.14170270885499658No Hit
ATCCTAACCTTACCAAATATTTACCTTTGGATAAAGGCATTAAACCTTAT7310.13866757720616132No Hit
TCCTCAGGCCATGCAGTGGAACTCCACCACATTCCACCAAGCTCTGCTAG7280.1380984900220047No Hit
GTTCCTGAACTGGAGCCACCAGCAGGAAAATATAGGCCCCTCACTCTGGG7210.1367706199256393No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTG7110.13487366264511724No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG7000.132787009636543No Hit
CCTCCCGCTTACAGACCACAAAATGCCCCTATCTTATCAACACTTCCGGA6810.1291827908035511No Hit
GGTTAGGATAAAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA6810.1291827908035511No Hit
CTATTCTGTGTTGGGGTGAGTTGATGAATCTGGCCACCTGGGTGGGAAGT6780.12861370361939448No Hit
GCACCTGTATTCCCATCCCATCATCCTGGGCTTTCGCAAGATTCCTATGG6760.1282343121632901No Hit
ATTCTGTGTTGGGGTGAGTTGATGAATCTGGCCACCTGGGTGGGAAGTAA6670.12652705061082023No Hit
CTTTTACACAATGTGGCTATCCTGCCTTGATGCCTTTATATGCATGTATA6650.12614765915471585No Hit
CTTGAGTGCTCCCCCTAGAAAATTGAGAGAAGTCCACCACGAGTCTAGAC6620.12557857197055922No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT6600.1251991805144548No Hit
GCACGGTTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTGTTGCTGTACA6440.12216404886561956No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT6370.12083617876925412No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTCAAATGTATA6340.1202670915850975No Hit
ATATAAGAGAGAAACTACACGCAGCGCTTCATTTTGTGGGTCACCATATT6260.11874952576067987No Hit
CCTCGGTAGTCCACAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGAT6160.11685256848015782No Hit
CCCTTAACTTCATGGGATATGTAATTGGCAGTTGGGGTACTTTACCACAG6060.1149556111996358No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTC6000.11381743683132256No Hit
CCACAAAATGCCCCTATCTTATCAACACTTCCGGAAACTACTGTTGTTAG5980.11343804537521816No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG5980.11343804537521816No Hit
CTTTAAAACTAGGCATTATTTACATACTCTGTGGAAGGCTGGCATTCTAT5970.11324834964716594No Hit
TATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAAA5920.11229987100690492No Hit
GGGTAAAGGTGCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA5910.11211017527885273No Hit
CCAACTTGTCCTGGCTATCGCTGGATGTGTCTGCGGCGTTTTATCATATT5900.1119204795508005No Hit
GCACGACTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTGTTGCTGTACA5890.11173078382274831No Hit
TTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACAC5820.11040291372638289No Hit
GGGCACATTGACAACAGTGCCAGCAGCGCCTCCTCCTGCCGTCACCAATC5790.10983382654222626No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTGCCAATTACATATCCCATGAA5770.10945443508612186No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCCTCCTGT5720.10850595644586083No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG5720.10850595644586083No Hit
CCCACAAAATGAAGCGCTGCGTGTAGTTTCTCTCTTATATAGAATGCCAG5710.10831626071780864No Hit
GGAACATATTGTACAAAAACTCAAGCAATGTTTTCGGAAATTGCCTGTAA5670.10755747780559982No Hit
CCTTTACCCCGTTGCACGGCAACGGTCAGGTCTCTGCCAAGTGTTTGCTG5620.1066089991653388No Hit
GGTAAATATTTGGTAAGGTTAGGATAAAACCTAGCAGGCATAATTAATTT5550.10528112906897337No Hit
GTCCAAGGGATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGAC5490.10414295470066015No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC5430.10300478033234692No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG5390.1022459974201381No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTCAGGCA5350.10148721450792927No Hit
CATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA5340.10129751877987708No Hit
CACACAATAGCTTGCCTGAGTGCTGTATGGTGAGGTGAACAATGTTCCGG5300.10053873586766827No Hit
ACACACAATAGCTTGCCTGAGTGCTGTATGGTGAGGTGAACAATGTTCCG5300.10053873586766827No Hit
GCACTCAAGTGTCCTGGCCAAAATTCGCAGTCCCCAACCTCCAATCACTC5300.10053873586766827No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)