FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002488369

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488369
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences345707
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA15270.44170352350400777No Hit
TCCCATGGCTGCTCGGGTGTGCTGCCAACTGGATCCTACGCGGGACGTCC15020.4344719661447411No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG11090.32079188445706913No Hit
CTCACCAACCTCTTGTCCTCCAATTTGTCCTGGCTATCGCTGGATGTGTC10480.30314688450045846No Hit
GTGTAAAAGGAGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT8800.25455081904618654No Hit
GTATACATGCATATAAAGGCATCAAGGCGGGATAACCACATTGTGTAAAA8580.24818704857003188No Hit
CATGTATACAATCTAAACAGGCTTTCACTTTCTCGCCAACTTACAAGGCC8480.24529442562632517No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC8210.2374843436783172No Hit
CGGTGGTTTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG7700.22273196666541317No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG7510.21723598307237055No Hit
ATCCTAACCTTACCAAATATCTGCCCTTGGATAAAGGCATTAAACCTTAT7240.20942590112436252No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC7020.20306213064820786No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN6690.19351647493397586No Hit
GGTTAGTAAGTGATTGGAGGTTGGGGACTGCGAATTTTGGCCAGGACACG6580.19033458969589856No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA6580.19033458969589856No Hit
ATTGACAACAGTGCCAGCAGCACCTCCTCCTGCCTCCACCAATCGGCAGT6500.1880204913409332No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG6400.18512786839722656No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTTTCCATGC6250.18078893398166654No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCCTCCTGT6170.17847483562670122No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG6010.17384663891677055No Hit
CCTTTTACACAATGTGGTTATCCCGCCTTGATGCCTTTATATGCATGTAT5960.17240032744491723No Hit
GCCACGGGAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGTTGTCAGTC5940.1718218028561759No Hit
CTTATATAAGAGCTTGGGCAAGACCTTGGGCAAGACCTGGTGGGCGTTCA5920.17124327826743455No Hit
GTTTAGATTGTATACATGCATATAAAGGCATCAAGGCGGGATAACCACAT5900.17066475367869324No Hit
ATTCTGTGTTGGGGTGAGTTGATGAATCTGGCCACCTGGGTGGGAAGTAA5620.16256540943631456No Hit
CTCTGTGGAAGGCTGGCATTCTATATAAAAGAGAAACTACACGCAGCGCT5580.1614083602588319No Hit
CTCCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCG5520.15967278649260788No Hit
TCCACAGAGTGTGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCA5460.15793721272638392No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA5430.1570694258432719No Hit
CTATTAATAATGTCCTCCTGTAAATGAATGTTAGGAAAGGAGGGAGTTTG5390.15591237666578925No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG5270.15244122913334124No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTGTTTGCTG5260.1521519668389706No Hit
ATATAAAAGAGAAACTACACGCAGCGCTTCATTTTGTGGGTCACCATATT5200.15041639307274657No Hit
GTGTGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG5190.15012713077837592No Hit
GTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC5120.14810229471778125No Hit
CTATTCTGTGTTGGGGTGAGTTGATGAATCTGGCCACCTGGGTGGGAAGT5100.14752377012903992No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTG5090.14723450783466926No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAAC5010.14492040947970392No Hit
GGGTAAAGGTGCAGGTATTGTTGACACAGAAAGGCCTTGTAAGTTGGCGA4950.1431848357134799No Hit
GCACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG4720.13653180294295458No Hit
ACTTATCGGGACTGACAACTCTGTTGTCCTCTCTCGGAAATACACCTCCT4700.13595327835421325No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC4670.13508549147110124No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA4630.13392844229361858No Hit
GCCATGGGAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGTTGTCAGTC4510.13045729476117057No Hit
ATTTACACACTCTGTGGAAGGCTGGCATTCTATATAAAAGAGAAACTACA4460.12901098328931726No Hit
TCCCGTGGCTGCTCGGGTGTGCTGCCAACTGGATCCTACGCGGGACGTCC4430.12814319640620525No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACATTCCACCAAGCTCTGCTAG4390.1269861472287226No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA4380.1266968849343519No Hit
ATATTTTCCTGCTGGTGGCTCCAGTTCCGGAACAGTAAACCTTGTTCCGA4360.1261183603456106No Hit
GGATTAAAGACAGGTACAGTAGAAGAATAAAGCCCAGTAAAGTTTCCCAC4320.12496131116812792No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT4220.12206868822442125No Hit
CCCCAAAAGACCGCCGTGTGGTGGGGTGAACCCTGGCCCGAATGCTCCCG4090.1183082783976026No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA4080.11801901610323193No Hit
TCCTTTGTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCG4070.11772975380886126No Hit
GGACTCGTGCAGGTCTTGCATGGTCCCGTGCTGGTAGTTGATGTTCCTGG4000.1157049177482666No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTGCGAACCACTGAACA4000.1157049177482666No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCCGATGGCC4000.1157049177482666No Hit
GTCCAAGGGATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGAC3960.11454786857078393No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG3950.11425860627641325No Hit
GTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAAA3940.1139693439820426No Hit
GGGTTCACCCCACCACACGGCGGTCTTTTGGGGTGGAGCCCTCAGGCTCA3940.1139693439820426No Hit
CTCCATGTTCGGTGCAGGGTCCCCAGTCCTCGAGAAGATTGACGATATGG3910.11310155709893059No Hit
GTATTAAAACTCAAACAATGTTTTCGGAAACTGCCTGTAAATAGACCTAT3910.11310155709893059No Hit
GTAGAGGCCCAAACGGCCCCGAGACGGGTCGTCCGCGGGATTCAGCGCCG3900.11281229480455994No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGCAGGGCTTTCCCCCACTGTT3890.11252303251018927No Hit
GGATGGGGCTTGGCCATAGGCCATCGGCGCATGCGTGGAACCTTTGTGGC3840.11107672103833593No Hit
GCCTAATCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC3840.11107672103833593No Hit
CATATAAAGGCATCAAGGCGGGATAACCACATTGTGTAAAAGGAGCAGCA3840.11107672103833593No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC3800.10991967186085327No Hit
AGTTAAGGGAGTAGCCCCAGCGTTTGGTTTTATTAGGGTTCAAATGTATA3800.10991967186085327No Hit
ATCTAAACAGGCTTTCACTTTCTCGCCAACTTACAAGGCCTTTCTGTGTC3780.10934114727211193No Hit
CTTTTATATAGAATGCCAGCCTTCCACAGAGTGTGTAAATAATGCCTAGT3780.10934114727211193No Hit
GAATACAAGTGCAGTTTCCGTCCGAAGGTTTTGTACAGCAACAAGAGGGA3750.10847336038899992No Hit
CCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAGTG3730.1078948358002586No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT3730.1078948358002586No Hit
GGTAATCTCCTCCCCCAACTCCTCCCAGTCCTTAAACAAACAGTCTTTGA3710.10731631121151726No Hit
TTATTATCCTGAACATGCAGTTAATCATTACTTCAAAACTAGGCATTATT3710.10731631121151726No Hit
GAACAAATGGCACTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCAC3700.10702704891714661No Hit
ATCCCAGAGTGAGGGGCCTATATTTTCCTGCTGGTGGCTCCAGTTCCGGA3680.10644852432840526No Hit
CATTTGAACCCTAATAAAACCAAACGCTGGGGCTACTCCCTTAACTTCAT3680.10644852432840526No Hit
GTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCGGGGCCG3670.1061592620340346No Hit
GCACGATTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTGTTGCTGTACA3670.1061592620340346No Hit
ATATAGGCCCCTCACTCTGGGATCTAGCAGAGCTTGGTGGAATGTTGTGG3640.1052914751509226No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG3590.10384516367906928No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA3590.10384516367906928No Hit
GGAGTAGGCTGTCTTCCTGACTGCCGATTGGTGGAGGCAGGAGGAGGTGC3570.10326663909032793No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCCGCTTACAGACCACCAAATGCC3570.10326663909032793No Hit
GTGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGGAT3550.1026881145015866No Hit
CTGCTAGATCCCAGAGTGAGGGGCCTATATTTTCCTGCTGGTGGCTCCAG3520.1018203276184746No Hit
GCCTGAGGATGACTGTCTCTTAGAGGTGGAGAGATGGGAGTAGGCTGTCT3510.10153106532410393No Hit
GATCATTAACCTAATCTCCTCCCCCAACTCCTCCCAGTCCTTAAACAAAC3480.10066327844099193No Hit
CCTCTGCTCTGTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCT3480.10066327844099193No Hit
ACATAGAGGTTCCTTGAGCAGGAATCGTGCAGGTCTTGCATGGTCCCGTG3470.10037401614662127No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)