FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002488370

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488370
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences324869
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN13880.4272491373445912No Hit
CCTTTAATCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAACAG11960.36814839212113193No Hit
CCTACCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA9110.28042072343005947No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG8580.2641064552173338No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA7940.244406206809514No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC7790.23978896108893125No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG7610.2342482662242319No Hit
CTATATAAGAGAGAAACTACACGCAGCGCTTCATTTTGTGGGTCACCATA7540.23209355155462663No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGC7430.2287075713595326No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT6820.2099307720958294No Hit
TTATTAGGGTTCAAATGTATACCCAAAGACAGAAGAAAATTGGTAATAGA6790.20900732295171284No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT6750.20777605742622413No Hit
GTACTAAAGATCAAGCAATGTTTTCGGAAACTGCCTGTAAATAGACCTAT6600.20315881170564135No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA6250.19238523835761492No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG6180.19023052368800963No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA6140.1889992581625209No Hit
GGTAAAAAGGGACTCAAGATGTTGTACAGACTTGGCCCCCAATACCACAT6050.18622891073017125No Hit
ATATAAGAGAGAAACTACACGCAGCGCTTCATTTTGTGGGTCACCATATT5810.1788413175772388No Hit
ATTGACAACAGTGCCAGCAGCGCCTCCTCCTGCCTCCACCAATCGGCAGT5670.17453188823802826No Hit
TCCTAGAAGTAGAGGACAAACGGGCAACATACCTTGGTAGTCCAGAAGAA5570.1714537244243064No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG5500.1692990097547011No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGGTCAAAGACC5490.16899119337332894No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTTTCGCCAACTTACAAGGCC5370.16529739679686273No Hit
CTCCTACCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCG5340.16437394765274618No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCTTAT5310.16345049850862964No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCCTCCTGT5300.16314268212725747No Hit
ATGACAAGGCACAGACGGGGATACCGCGTAAAGAGAGGTGCGCCCCGTGG5210.1603723346949078No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG5030.15483163983020848No Hit
GCTCTATATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCA4960.1526769251606032No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACA4960.1526769251606032No Hit
CCCTCATAAAACCAAGCGTTGGGGCTACTCCCTTAACTTCATGGGATATG4940.15206129239785882No Hit
GGGTAAAGGTGCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA4920.15144565963511447No Hit
GTTTCCGTCCGAAGGTTTTGTACAGCAACAAGAGGGAAACATAGAGGTTC4910.15113784325374227No Hit
CTATTAATAATGTCCTCCTGTAAATGAATGTTAGGAAAGGAGGGAGTTTG4870.14990657772825353No Hit
CTTTAGTACAATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACAT4800.14775186305864824No Hit
GCACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG4790.14744404667727606No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA4760.14652059753315952No Hit
ATATTAACGTTGACATAGCTGACTACTAATTCCCTGGATGCTGGGTCTTC4630.14251898457532114No Hit
CCTTTTCATCTGCCGGACCGTGTGCACTTCGCTTCACCTCTGCACGTCGC4620.14221116819394894No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTGTTTGCTG4570.14067208628708802No Hit
TCCCATGGCTGCTCGGATGTGCTGCCAACTGGATCCTGCGCGGGACGTCC4480.13790173885473836No Hit
GACAGAAACTGCACTTGTATTCCCATCCCATCATCCTGGGCTTTCGCAAG4470.1375939224733662No Hit
ACCACCAGCACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAAC4450.13697828971062181No Hit
GCCATGGGAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGTTGTCAGTC4440.13667047332924964No Hit
CATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA4390.13513139142238872No Hit
GTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTAT4370.13451575865964435No Hit
TCCTCAGGCCATGCAGTGGAACTCCACCACATTCCACCAAGCTCTGCTAG4290.1320532276086669No Hit
GATGACAAGGCACAGACGGGGATACCGCGTAAAGAGAGGTGCGCCCCGTG4290.1320532276086669No Hit
GTGCTGGTGCACAGACCAATTTATGCCTACAGCCTCCTAGGTCAAAGACC4280.13174541122729472No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAAC4250.13082196208317814No Hit
GTCTTTAAACAAACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA4250.13082196208317814No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG4210.12959069655768943No Hit
ATATTTTCCTGCTGGTGGCTCCAGTTCCGGAACAGTAAACCCTGTTCCGA4140.12743598188808414No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTC4000.12312655254887354No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA3960.1218952870233848No Hit
CTCCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCG3950.12158747064201263No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT3940.12127965426064044No Hit
CTTTTACACAATGTGGCTATCCTGCCTTGATGCCTTTATATGCATGTATA3900.12004838873515171No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA3890.11974057235377951No Hit
TTCTATTCGTGATCTCCTCGACACCGCCTCTGCTCTATATCGGGAGGCCT3880.11943275597240734No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTG3880.11943275597240734No Hit
GTTTTATTAGGGTTCAAATGTATACCCAAAGACAGAAGAAAATTGGTAAT3810.11727804130280205No Hit
GTATGGATCGGTAGAGGAGCCATAAAGGTTCCACGCATGCGCCGATGGCC3790.11666240854005767No Hit
GTGTAAACAATATCTGCACCTTTACCCCGTTGCCCGGCAACGGTCAGGTC3780.1163545921586855No Hit
ACATAGAGGTTCCTTGAGCAGGAATCGTGCAGGTCTTGCATGGTCCCGTG3770.11604677577731332No Hit
GGTTAGGATAAAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA3710.11419987748908021No Hit
GTACAATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCC3700.11389206110770803No Hit
GTAGAGGAGCCATAAAGGTTCCACGCATGCGCCGATGGCCTATGGCCAAG3680.11327642834496367No Hit
TCCTTTGTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCG3680.11327642834496367No Hit
GAACAAATGGCACTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCAC3670.11296861196359148No Hit
CCACCAGCACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACC3660.11266079558221931No Hit
CGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCTT3560.10958263176849745No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT3560.10958263176849745No Hit
CCCAAAGACAGAAGAAAATTGGTAATAGAGGTAAAAAGGGACTCAAGATG3530.1086591826243809No Hit
CCTAGGTCAAAGACCTTTAATCTAATCTCCTCCCCCAACTCCTCCCAGTC3520.10835136624300873No Hit
CTTTAATACAATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACAT3520.10835136624300873No Hit
GCTTGTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGT3500.10773573348026434No Hit
CTTTTTACCTCTATTACCAATTTTCTTCTGTCTTTGGGTATACATTTGAA3490.10742791709889216No Hit
CTCCATGTTCGGTGCAGGGTCCCCAGTCCTCGAGAAGATTGACGATATGG3480.10712010071751998No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT3480.10712010071751998No Hit
GCCTAATCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC3470.1068122843361478No Hit
GCTGTTGGTTGATGATCCTGGAAGTAGAGGACAAACGGGCAACATACCTT3470.1068122843361478No Hit
GGAAAATATAGGCCCCTCACTCTGGGATCTAGCAGAGCTTGGTGGAATGT3440.10588883519203125No Hit
GGATGGGGCTTGGCCATAGGCCATCGGCGCATGCGTGGAACCTTTATGGC3420.10527320242928687No Hit
ATATAGGCCCCTCACTCTGGGATCTAGCAGAGCTTGGTGGAATGTGGTGG3390.10434975328517034No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTCAGGCA3390.10434975328517034No Hit
GGATTAAAGACAGGTACAGTAGAAGAATAAAGCCCAGTAAAGTTTCCCAC3350.10311848775968159No Hit
TTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACAC3310.10188722223419286No Hit
CCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAAGAGTCCTCTTATGT3300.10157940585282067No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAACATTCATTTACAGG3290.1012715894714485No Hit
GGTATAAAGGGACTCAAGATGTTGTACAGACTTGGCCCCCAATACCACAT3290.1012715894714485No Hit
GTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCCGCCTGTAACACG3270.10065595670870411No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTGCGAACCACTGAACA3270.10065595670870411No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)