FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488372

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488372
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences475775
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTGGTACGCAGACCAATTTATGCCTACAGCCTCCTAATACAAAGACC14720.30938994272502757No Hit
CTTATATAGAATGCCAGCCTTCCAAAGGGTATGCAAATAGTGCCTAGCTT11980.25179969523409174No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN10380.21817035363354528No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTTTCCATGC9840.20682045084336081No Hit
CTCTTACTGTAAACGAAAAAAGGAGATTAAAGTTAGTTATGCCTGCTAGA9800.20597971730334716No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC9750.20492880037833008No Hit
ATTGACAACAGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT9420.1979927486732174No Hit
CTATATAAGAGAGAAACTACACGCAGCGCTTCATTTTGTGGGTCACCATA9180.1929483474331354No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG8760.18412064526299196No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGGCATACC8740.18370027849298512No Hit
GTATAAAGAGTTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG8130.1708790920077768No Hit
CATGTATACAAGCTAAGCAGGCCTTCACTTTCTCGCCAACTTATAAGGCC7760.16310230676265042No Hit
CTTTAACCCTGAGTGGCAAACTCCCTCTTTTCCTGATATACATTTGCAGG7740.16268193999264358No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGGCCGCCGTG7620.16015973937260258No Hit
GTATACATGCATACAAAGGTAGTAATGCAGGATAACCACATTGTGTAAAA7390.15532552151752405No Hit
CTCTTATATAGAATGCCAGCCTTCCAAAGGGTATGCAAATAGTGCCTAGC7270.15280332089748305No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC7210.15154222058746258No Hit
ATCCAGCATCCAGGGAATTAGTAGTCAGCTATGTCAATGTTAATATGGGC7170.1507014870474489No Hit
ATATGATCCTGTGGTAAAGTACCCCAACCTCCAATTACATATCCCATAAA7160.15049130366244548No Hit
GTTCAGGATAATAAGGTTTAATGCCTTTATCTAAGGGCAAATATTTGGTA7130.14986075350743525No Hit
ATATAAGAGAGAAACTACACGCAGCGCTTCATTTTGTGGGTCACCATATT7080.14880983658241817No Hit
GGATAATAAGGTTTAATGCCTTTATCTAAGGGCAAATATTTGGTAAGGTT6730.1414534181072986No Hit
CTTATAAGGCCTTTCTGTGTAAACAATATCTGACCCTTTACCCCGTTGCC6700.14082286795228838No Hit
CTTCTAATGTACCTCACTTTAACCCTGAGTGGCAAACTCCCTCTTTTCCT6690.14061268456728496No Hit
GGATCATATTGTACTGAAAATCAAGCAATGTTTTCGAAAACTCCCTGTTA6500.13661920025222005No Hit
CTTTTACACAATGTGGTTATCCTGCATTACTACCTTTGTATGCATGTATA6450.135568283327203No Hit
CATTTGAACCCTCATAAAACCAAGCGTTGGGGCTACTCCCTTAATTTTAT6430.13514791655719616No Hit
GTCTTTGAAGTACGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA6420.13493773317219274No Hit
CAGTAAGAGGGCCCACAAATTGTTGACATCTATCAATAATGTCCTCCTGC6420.13493773317219274No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTGTTACCAATTTTCTT6340.13325626609216543No Hit
TCCTAATACAAAGACCTTCAACCTAATCTCCTCCCCCAACTCCTCCCAGT6310.13262571593715516No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGATTTCTTTCCTTCTATTCG6300.13241553255215174No Hit
GGGTTAAAGTGAGGTACATTAGAAGAATAAAGCCCAGTAAAGTTTCCCAC6200.1303136987021176No Hit
ACCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAACAGTCTTTG6150.12926278177710052No Hit
TCCAAAGGGTATGCAAATAGTGCCTAGCTTTGAAGTAATGATTAACTATA6150.12926278177710052No Hit
ACATAGAGGTGCCTTGAGCAGGAGTCGTGCAGGTCTTGCATGGTCCCGTG6080.12779149808207663No Hit
CCCTTAATTTTATGGGATATGTAATTGGAGGTTGGGGTACTTTACCACAG5940.12484893069202879No Hit
AATTAAGGGAGTAGCCCCAACGCTTGGTTTTATGAGGGTTCAAATGTATA5590.11749251221690926No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG5490.11539067836687508No Hit
ATGCTGGATCGTCCAAATTACTTCCCACCCAGGTAGCCAGATTCATCAAC5420.11391939467185119No Hit
TATTAACATTGACATAGCTGACTACTAATTCCCTGGATGCTGGATCGTCC5420.11391939467185119No Hit
ATGTTAATATGGGCCTAAAAATTAGACAACTGTTGTGGTTTCACATTTCT5350.11244811097682729No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAG5320.11181756082181704No Hit
ATATTGTACTGAAAATCAAGCAATGTTTTCGAAAACTCCCTGTTAATCGA5310.11160737743681362No Hit
ACTATATGTTCAGGATAATAAGGTTTAATGCCTTTATCTAAGGGCAAATA5210.10950554358677947No Hit
CATATAGGCCATCAAATGCCCCTATCTTATCAACACTTCCGGAAACTACT5180.10887499343176922No Hit
GATATACATTTGCAGGAGGACATTATTGATAGATGTCAACAATTTGTGGG5130.10782407650675213No Hit
GTACAATATGATCCTGTGGTAAAGTACCCCAACCTCCAATTACATATCCC5050.10614260942672482No Hit
ATCTAAGGGCAAATATTTGGTAAGGTTAGGAAAAAATCTAGCAGGCATAA5010.10530187588671115No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTAATGTACCTCAC5010.10530187588671115No Hit
CCTGTATTCCCATCCCATCATCCTGGGCTTTCGCAAAATTCCTATGGGAG5000.10509169250170773No Hit
TTTGATGGCCTATATGCGGGGGGTGTACGGATCCACACCCCGAAGGACAC4950.10404077557669067No Hit
ATCATATTGTACTGAAAATCAAGCAATGTTTTCGAAAACTCCCTGTTAAT4890.10277967526667016No Hit
GGTTAGGAAAAAATCTAGCAGGCATAACTAACTTTAATCTCCTTTTTTCG4860.10214912511165994No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA4860.10214912511165994No Hit
GTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTC4820.10130839157164627No Hit
CTAGTAAACTGAGCCATGAGAAACGGACTGAGGCCCACTCCCATAGGAAT4760.10004729126162576No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)