FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488374

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488374
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences608011
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN72261.1884653402652254No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACT21170.3481844900832386No Hit
TCCACAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA19260.31677058474271025No Hit
CTTATATAATATACCCGCCTTCCACAGAGTGTGTAAATAATGTCTGGTTT16620.27335031767517365No Hit
CCCTTATCTAATGGCAAATATTTGGTAACATTTGGATAAAACCTAGCAGG16240.2671004307487858No Hit
GTTTAATACCCTTATCTAATGGCAAATATTTGGTAACATTTGGATAAAAC15220.2503244184726921No Hit
CTCTTATATAATATACCCGCCTTCCACAGAGTGTGTAAATAATGTCTGGT14690.24160747091746693No Hit
GTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT13560.2230222808468926No Hit
AGATAAGGGTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACT13160.21644345250332642No Hit
AGTCTGTACAGCACCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT12870.211673801954241No Hit
GTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACC12700.20887779990822536No Hit
GGATAATAAGGTTTAATACCCTTATCTAATGGCAAATATTTGGTAACATT12500.20558838573644225No Hit
ATATAATATACCCGCCTTCCACAGAGTGTGTAAATAATGTCTGGTTTGGA12500.20558838573644225No Hit
CTCTATGACCGTTTGACTTCCAAAAGTGAGACAAGAAATGTGAAACCACA12390.20377920794196158No Hit
CACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACTTACAAT11970.1968714381812171No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC11380.18716766637445703No Hit
GTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGG11120.18289142795113905No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC10940.1799309551965343No Hit
CTTACAATTAATGAGAAAAGACGACTGCAATTGATTATGCCTGCTAGGTT10670.17549024606462713No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG10610.1745034218130922No Hit
CACTAATATGGGCCTAAAGTTCAGGCAATTATTGTGGTTTCACATTTCTT10350.17022718338977422No Hit
ATCCAAATGTTACCAAATATTTGCCATTAGATAAGGGTATTAAACCTTAT10030.16496412071492128No Hit
TAATATACCCGCCTTCCACAGAGTGTGTAAATAATGTCTGGTTTGGAAGT9360.15394458323944796No Hit
CATTTAAACCCTAACAAAACAAAAAGATGGGGTTACTCTTTAAATTTCAT9310.15312222969650222No Hit
GTGTAAAAGGGGCAGCAAACCCCAAAAGACCCACAATACGTTGACAGACT9260.15229987615355642No Hit
TTCTTCTACTGTACCTGTCTTTAACCCACATTGGAAAACACCCTCTTTTC8260.13585280529464105No Hit
TTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACCAGACATTATT8160.13420809820874952No Hit
GTTCTGGATAATAAGGTTTAATACCCTTATCTAATGGCAAATATTTGGTA7960.13091868403696644No Hit
CAATAGGCCTGTTAACAGGAAGTTTCCTAAAACATTCTTTGATTTTCTGT7650.12582009207070266No Hit
AATATACATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGG7540.12401091427622196No Hit
CTACTGTACCTGTCTTTAACCCACATTGGAAAACACCCTCTTTTCCTAAT7510.12351750215045451No Hit
GGCATAATCAATTGCAGTCGTCTTTTCTCATTAATTGTAAGTGGGCCTAC7450.12253067789891958No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCTTTGTATGCCTGTATT7220.11874785160136905No Hit
CATTTGGATAAAACCTAGCAGGCATAATCAATTGCAGTCGTCTTTTCTCA7130.11726761522406667No Hit
ATCTAGAAGATCTCGTACTGAAGGAAAGAAGTCAGTAGGCAAAAAAGAGA6860.1128269060921595No Hit
ATAATATACCCGCCTTCCACAGAGTGTGTAAATAATGTCTGGTTTGGAAG6740.11085325758908968No Hit
GGTTTAATACCCTTATCTAATGGCAAATATTTGGTAACATTTGGATAAAA6650.1093730212117873No Hit
ATCCAGAACATCTAGTTAATCATTACTTCCAAACCAGACATTATTTACAC6640.10920855050319814No Hit
CAATTAATGAGAAAAGACGACTGCAATTGATTATGCCTGCTAGGTTTTAT6590.10838619696025237No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAAACTTCCACCAAACTCTGCAAG6570.10805725554307406No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA6530.10739937270871744No Hit
GTACAGCACCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC6450.1060836070400042No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTC6400.10526125349705844No Hit
TCCTTGGACTCATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACTG6320.1039454878283452No Hit
GTGTAAATGTATATTAGGAAAAGAGGGTGTTTTCCAATGTGGGTTAAAGA6210.10213631003386452No Hit
CTCATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACTGTACCTGTC6180.10164289790809705No Hit
GTCATAGAGTATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCAGC6170.10147842719950789No Hit
GTCTTTGGGTGTACATTTAAACCCTAACAAAACAAAAAGATGGGGTTACT6120.10065607365656214No Hit
CTTTAGGACATGGACATTGACCCTTATAAAGAATTTGGAGCTACTGTGGA6090.10016266153079467No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)