FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488375

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488375
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences608011
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN72101.185833808927799No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACT20210.33239530205867984No Hit
TCCACAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA18610.30607998868441527No Hit
GTTTAATACCCTTATCTAATGGCAAATATTTGGTAACATTTGGATAAAAC17550.2886460935739649No Hit
CTTATATAATATACCCGCCTTCCACAGAGTGTGTAAATAATGTCTGGTTT15690.2580545417763823No Hit
CCCTTATCTAATGGCAAATATTTGGTAACATTTGGATAAAACCTAGCAGG15250.2508178305984596No Hit
CTCTTATATAATATACCCGCCTTCCACAGAGTGTGTAAATAATGTCTGGT14920.2453902972150175No Hit
GTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACC14660.2411140587916995No Hit
GTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT14090.23173922840211775No Hit
GGATAATAAGGTTTAATACCCTTATCTAATGGCAAATATTTGGTAACATT14070.2314102869849394No Hit
AGATAAGGGTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACT13880.2282853435217455No Hit
CTCTATGACCGTTTGACTTCCAAAAGTGAGACAAGAAATGTGAAACCACA12790.21035803628552774No Hit
ATATAATATACCCGCCTTCCACAGAGTGTGTAAATAATGTCTGGTTTGGA11970.1968714381812171No Hit
AGTCTGTACAGCACCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT11880.19539120180391473No Hit
GTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGG11820.1944043775523798No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG11090.1823980158253716No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC10850.1784507188192319No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC10480.1723653026014332No Hit
CACTAATATGGGCCTAAAGTTCAGGCAATTATTGTGGTTTCACATTTCTT10460.1720363611842549No Hit
CACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACTTACAAT10430.17154294905848744No Hit
CATTTAAACCCTAACAAAACAAAAAGATGGGGTTACTCTTTAAATTTCAT10150.1669377692179911No Hit
ATCCAAATGTTACCAAATATTTGCCATTAGATAAGGGTATTAAACCTTAT9990.16430623788056467No Hit
CTTACAATTAATGAGAAAAGACGACTGCAATTGATTATGCCTGCTAGGTT9960.16381282575479722No Hit
TTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACCAGACATTATT9370.15410905394803714No Hit
GTTCTGGATAATAAGGTTTAATACCCTTATCTAATGGCAAATATTTGGTA8700.14308951647256382No Hit
CAATAGGCCTGTTAACAGGAAGTTTCCTAAAACATTCTTTGATTTTCTGT8630.14193822151243976No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCTTTGTATGCCTGTATT8320.13683962954617596No Hit
TAATATACCCGCCTTCCACAGAGTGTGTAAATAATGTCTGGTTTGGAAGT8160.13420809820874952No Hit
TTCTTCTACTGTACCTGTCTTTAACCCACATTGGAAAACACCCTCTTTTC8010.1317410375799122No Hit
GTGTAAAAGGGGCAGCAAACCCCAAAAGACCCACAATACGTTGACAGACT7980.13124762545414476No Hit
GGTTTAATACCCTTATCTAATGGCAAATATTTGGTAACATTTGGATAAAA7450.12253067789891958No Hit
CATTTGGATAAAACCTAGCAGGCATAATCAATTGCAGTCGTCTTTTCTCA7310.12022808797867145No Hit
AATATACATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGG7260.11940573443572568No Hit
GGCATAATCAATTGCAGTCGTCTTTTCTCATTAATTGTAAGTGGGCCTAC6870.11299137680074868No Hit
CTACTGTACCTGTCTTTAACCCACATTGGAAAACACCCTCTTTTCCTAAT6810.11200455254921375No Hit
ATCTAGAAGATCTCGTACTGAAGGAAAGAAGTCAGTAGGCAAAAAAGAGA6810.11200455254921375No Hit
CAATTAATGAGAAAAGACGACTGCAATTGATTATGCCTGCTAGGTTTTAT6790.11167561113203545No Hit
GTCTTTGGGTGTACATTTAAACCCTAACAAAACAAAAAGATGGGGTTACT6770.11134666971485713No Hit
GTTAACAGGAAGTTTCCTAAAACATTCTTTGATTTTCTGTTTGATGTGAT6680.10986643333755475No Hit
GTACAGCACCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC6550.10772831412589574No Hit
GTGTAAATGTATATTAGGAAAAGAGGGTGTTTTCCAATGTGGGTTAAAGA6540.10756384341730658No Hit
GTCATAGAGTATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCAGC6530.10739937270871744No Hit
ATCCAGAACATCTAGTTAATCATTACTTCCAAACCAGACATTATTTACAC6500.10690596058294999No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAAACTTCCACCAAACTCTGCAAG6300.1036165464111669No Hit
ATCCAGCATCTAGGGACCTAGTAGTCAGTTATGTCAACACTAATATGGGC6300.1036165464111669No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA6220.10230078074245368No Hit
ATAATATACCCGCCTTCCACAGAGTGTGTAAATAATGTCTGGTTTGGAAG6130.1008205443651513No Hit
GATTATGCCTGCTAGGTTTTATCCAAATGTTACCAAATATTTGCCATTAG6100.10032713223938382No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)