FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488376

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488376
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences470766
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN30120.6398083124099871No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACCGAATGTTCAGG15500.32925062557618856No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGACAAAGGCATTAAACCCTAT14600.31013284731692603No Hit
ATATAAGACCTTGGGCAAGACCTGGCGGGCGTTCACGGTGGTCTCCATGC12540.26637437707905837No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACGAGAAAAACCCC10220.21709299312184824No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC9920.21072040036876072No Hit
ATTGACAACAGTGCCAGTAGCACCTCCTCCTGCCTCCACCAATCGGCAGT9550.20286086930661942No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA9450.2007366717222569No Hit
GTATACATGCATATAAAGGCATTAAGGCAGGATAGCCACATTGTGTAAAA8490.18034437491237684No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG7900.16781160916463805No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTCTGACATACT7240.15379190510784552No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCGCAA7220.15336706559097302No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTGGACAAAGGCATTAAAC6990.14848141114693925No Hit
GTCTTATATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT6830.14508269501195922No Hit
CTGTAAGAGGGCCCACAAATTGTTCACATCTATTAATAATGTCCTCCTGT6670.1416839788769792No Hit
CCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT6650.14125913936010673No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG6560.13934736153418045No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC6490.1378604232251267No Hit
GCTTTAGTTTTTGTACAATATGTTCTTGCGGTAAAGTACCCCAACTTCCA6480.13764800346669046No Hit
CTTATATAGAATTCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT6230.13233750950578418No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA6220.13212508974734793No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT6160.13085057119673044No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACCGAA6090.1293636328876767No Hit
CTCCCATAGGAATCTTACGAAAGCCCAAGATGATGGGATGGGAATACAAG6010.12766427482018666No Hit
GTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC5880.12490281796051542No Hit
AACTCAAGCAATGTTTTCGAAAACTGCCTGTAAATAGACCTATTGATTGG5760.1223537808592804No Hit
TTCTATGGGTCACCATATTCTTGGGAACAAGAGCTACAGCATGGGAGGTT5730.12171652158397166No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG5590.11874264496586413No Hit
GGATAATAGGGTTTAATGCCTTTGTCCAAGGGCAAATATTTGGTAAGGTT5450.11576876834775664No Hit
GGGTAAAGGTTCATATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA5290.11237005221277661No Hit
CCCACGTGTCCTGGCCAAAATTCGCAGTCCCCAACCTCCAATCACTCACC5280.11215763245434036No Hit
ATATGAACCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTG5260.11173279293746788No Hit
ATCTTATCCACACTTCCGGAAACTACTATTGTTAGACGACGAGGCAGGTC5250.11152037317903163No Hit
CTCTTATATAAGACCTTGGGCAAGACCTGGCGGGCGTTCACGGTGGTCTC5210.11067069414528663No Hit
CTTTTACACAATGTGGCTATCCTGCCTTAATGCCTTTATATGCATGTATA5130.1089713360777966No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA5120.10875891631936035No Hit
GGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCATTAAC5120.10875891631936035No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGAGTGGAAAACTCCCTCCTTTC5000.10620987921812536No Hit
ATGTTAGGAAAGGAGGGAGTTTTCCACTCAGGATTAAAGACAGGTACAGT4970.1055726199428166No Hit
AACTAAAGCAATGTTTTCGAAAACTGCCTGTAAATAGACCTATTGATTGG4950.10514778042594411No Hit
CTATTATCCTGAACATTCGGTTAATCATTACTTCAAAACTAGGCATTATT4890.1038732618753266No Hit
TTATTAATAGATGTGAACAATTTGTGGGCCCTCTTACAGTTAATGAAAAA4890.1038732618753266No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGCACACCTCACCATACAG4880.10366084211689035No Hit
GGTTCATATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTG4830.10259874332470909No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTGTTTGCTG4820.10238632356627284No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)