FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488377

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488377
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences470766
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN30060.6385337938593696No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACCGAATGTTCAGG16790.35665277441446497No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGACAAAGGCATTAAACCCTAT14260.3029105755300935No Hit
ATATAAGACCTTGGGCAAGACCTGGCGGGCGTTCACGGTGGTCTCCATGC10540.22389042539180826No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACGAGAAAAACCCC9920.21072040036876072No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA9420.20009941244694815No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC8860.18820390597451814No Hit
ATTGACAACAGTGCCAGTAGCACCTCCTCCTGCCTCCACCAATCGGCAGT8720.1852300293564106No Hit
GTATACATGCATATAAAGGCATTAAGGCAGGATAGCCACATTGTGTAAAA8670.18416793056422937No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG8380.1780077575695781No Hit
GTCTTATATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT7650.16250111520373178No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTCTGACATACT7320.15549126317533551No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG7110.15103044824817424No Hit
CCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT6970.14805657163006675No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCGCAA6970.14805657163006675No Hit
CTGTAAGAGGGCCCACAAATTGTTCACATCTATTAATAATGTCCTCCTGT6660.14147155911854298No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTGGACAAAGGCATTAAAC6490.1378604232251267No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG6310.13403686757327418No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC6310.13403686757327418No Hit
CTCCCATAGGAATCTTACGAAAGCCCAAGATGATGGGATGGGAATACAAG6240.13254992926422043No Hit
GCTTTAGTTTTTGTACAATATGTTCTTGCGGTAAAGTACCCCAACTTCCA6210.13191266998891168No Hit
ATATGAACCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTG6030.1280891143370592No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT5960.12660217602800541No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA5930.12596491675269667No Hit
CTTATATAGAATTCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT5910.12554007723582417No Hit
TTCTATGGGTCACCATATTCTTGGGAACAAGAGCTACAGCATGGGAGGTT5700.1210792623086629No Hit
GTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC5560.1181053856905554No Hit
AACTCAAGCAATGTTTTCGAAAACTGCCTGTAAATAGACCTATTGATTGG5550.11789296593211913No Hit
GGGTAAAGGTTCATATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA5230.11109553366215912No Hit
GGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCATTAAC5230.11109553366215912No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGCACACCTCACCATACAG5180.11003343486997785No Hit
GGATAATAGGGTTTAATGCCTTTGTCCAAGGGCAAATATTTGGTAAGGTT5180.11003343486997785No Hit
GCTTTAGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCAGTGG5130.1089713360777966No Hit
GTATAAAGAATTTGGAGCTTCAGTGGAGTTACTCTCTTTTTTGCCTTCTG5000.10620987921812536No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACCGAA4970.1055726199428166No Hit
TTATTAATAGATGTGAACAATTTGTGGGCCCTCTTACAGTTAATGAAAAA4930.1047229409090716No Hit
GGTTCATATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTG4870.10344842235845408No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGAGTGGAAAACTCCCTCCTTTC4860.10323600260001785No Hit
AACTAAAGCAATGTTTTCGAAAACTGCCTGTAAATAGACCTATTGATTGG4830.10259874332470909No Hit
GTCCAAGGGATACTAACATTGAGATCCCCGAGATTGAGATCTTCTGCGAC4810.1021739038078366No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTGTTTGCTG4770.10132422477409159No Hit
ATCTTATCCACACTTCCGGAAACTACTATTGTTAGACGACGAGGCAGGTC4750.10089938525721909No Hit
CTTTTACACAATGTGGCTATCCTGCCTTAATGCCTTTATATGCATGTATA4720.10026212598191032No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)