FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002488379

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488379
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences598662
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC17420.29098222369216686No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN16610.2774520514079731No Hit
CTCTACGACTGTTTCTCTTCCAAAAGTGAGACAAGAAATATGAAACCACA15550.25974590002371956No Hit
ATAATATGCCCGCCTTCCATAGGGTATGTAAATAGTGTCTGGTTTGGAAG15450.25807550838369564No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT13680.22850957635527225No Hit
ATGTAAATAGTGTCTGGTTTGGAAGTAATGATTAACTACATTATCTGGAT13580.22683918471524833No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTTTTGTCAACGAGAAAAACCCC12940.21614867821909525No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTCGGGGGAGCTCCCGTG12420.20746264169097087No Hit
CTACTATACCTGTCTTTAATCCTAACTGGAAAACGCCATCTTTTCCTGAT12320.20579225005094695No Hit
TCCATAGGGTATGTAAATAGTGTCTGGTTTGGAAGTAATGATTAACTACA12280.20512409339493737No Hit
CCTCCAGCCTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA11030.1842441978946384No Hit
GTCTTATATAAGAGGACTATTGGACTCTCTGCAATGTCAACGACCGACCT10000.167039164002392No Hit
CTTTATACCTCTGTGACCAATTTTCTTTTGTCTTTGGGTATACATTTAAA9980.16670508567438722No Hit
ATACTAATATGGGCTTAAAGTTCAGACAATTATTGTGGTTTCATATTTCT9860.16470061570635852No Hit
CTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAATCCCAACAAA9520.1590212841302772No Hit
ACATTATCTGGATAATAAGGTTTTATACCTTTCTCTAGGGGTAAATATTT9500.1586872058022724No Hit
TGTAAATAGTGTCTGGTTTGGAAGTAATGATTAACTACATTATCTGGATA9370.15651569667024132No Hit
GTATGTAAATAGTGTCTGGTTTGGAAGTAATGATTAACTACATTATCTGG9050.15117044342216476No Hit
ATTTGTAGGTCCTCTAACAGTAAATGAAAAACGAAGATTAAACTTAGTCA8950.14950005178214085No Hit
GTTTTATACCTTTCTCTAGGGGTAAATATTTCGTAGAGATGGGAAAAAAC8900.1486648559621289No Hit
ATATAAGACCTTGGGCAAGAATTGGTGGGCGTTCACGGTGGTTTCCATGC8650.14448887686206907No Hit
GGATATCCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAAT8560.14298552438604756No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGAGGGACGTCCTTTGTCTA8560.14298552438604756No Hit
CTTATATAATATGCCCGCCTTCCATAGGGTATGTAAATAGTGTCTGGTTT8320.13897658444999014No Hit
ACTATACCTGTCTTTAATCCTAACTGGAAAACGCCATCTTTTCCTGATAT8250.1378073103019734No Hit
CTTTTACACAATGTGGATATCCTGCTTTAATGCCTCTGTATGCGTGTATT8240.137640271137971No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTCCGGGAGAGAACGACAGAAT8030.13413244869392077No Hit
CTCTTATATAATATGCCCGCCTTCCATAGGGTATGTAAATAGTGTCTGGT7960.13296317454590403No Hit
CTACTATACCTGTCTTTAATCCTAACTGGAAAACTCCATCTTTTCCTGAT7830.13079166541387294No Hit
GTCTTTGGGTATACATTTAAATCCCAACAAAACAAAAAGATGGGGATATT7590.12678272547781552No Hit
TATCCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCT7480.12494529467378922No Hit
ACTGTGCAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCAGTAAA7440.12427713801777965No Hit
ATACCTCTGTGACCAATTTTCTTTTGTCTTTGGGTATACATTTAAATCCC7380.1232749030337653No Hit
GGATAATAAGGTTTTATACCTTTCTCTAGGGGTAAATATTTCGTAGAGAT7360.12294082470576051No Hit
CCTAATAGTCAGTTATGTCAATACTAATATGGGCTTAAAGTTCAGACAAT7320.12227266804975094No Hit
CTCCAAAAGACACCAAATACTCTACGACTGTTTCTCTTCCAAAAGTGAGA7270.121437472229739No Hit
TTATTGACAAATGTGAACAATTTGTAGGTCCTCTAACAGTAAATGAAAAA7120.11893188476970311No Hit
GGAATATGATAAAACGCCGCAGACACATCCAGCGATAGCCAGGACAAATT7100.11859780644169832No Hit
ATTGACAAATGTGAACAATTTGTAGGTCCTCTAACAGTAAATGAAAAACG6940.11592517981766005No Hit
CTCTAACAGTAAATGAAAAACGAAGATTAAACTTAGTCATGCCTGCTAGG6890.11508998399764808No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA6890.11508998399764808No Hit
CATTTAAATCCCAACAAAACAAAAAGATGGGGATATTCCCTAAATTTCAT6820.11392070984963135No Hit
TTGACATACTTTCCAATCAATAGGCCGGTTAACAGGGAGTTTTCTAAAAC6760.11291847486561699No Hit
GGGTATGTAAATAGTGTCTGGTTTGGAAGTAATGATTAACTACATTATCT6760.11291847486561699No Hit
GTACGGGACCCTGCCGAACCTGCACGACTCTTGCTCAAAGAACCTCTATG6720.11225031820960743No Hit
GTATAAAACCTTATTATCCAGATAATGTAGTTAATCATTACTTCCAAACC6660.11124808322559307No Hit
ACTATACCTGTCTTTAATCCTAACTGGAAAACTCCATCTTTTCCTGATAT6550.10941065242156676No Hit
ATGTGGATATCCTGCTTTAATGCCTCTGTATGCGTGTATTCAATCTAAGC6550.10941065242156676No Hit
GTATGCGTGTATTCAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACA6550.10941065242156676No Hit
GCCTAATCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC6540.10924361325756436No Hit
GTTAGAGGACCTACAAATTGTTCACATTTGTCAATAATGTCCTGGTGCAA6540.10924361325756436No Hit
GTGTAAACAATACCTGAACCTTTACCCCGTTGCCCGGCAACGGCCAGGTC6500.10857545660155479No Hit
CTATTTACATACCCTATGGAAGGCGGGCATATTATATAAGAGAGAAACTA6460.10790729994554524No Hit
ATATAAGAGAGAAACTACGCGTAGCGCCTCATTTTGTGGGTCACCTTATT6420.10723914328953567No Hit
CTGTTAGAGGACCTACAAATTGTTCACATTTGTCAATAATGTCCTGGTGC6310.10540171248550935No Hit
GCTTTAATGCCTCTGTATGCGTGTATTCAATCTAAGCAGGCTTTCACTTT6130.10239500753346631No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCAGAGAGTCCAA6040.10089165505744477No Hit
TCCCAATGTTAGTATTCCTTGGACTCACAAGGTGGGGAATTTTACGGGGC6000.10022349840143521No Hit
GTGGATATCCTGCTTTAATGCCTCTGTATGCGTGTATTCAATCTAAGCAG5990.1000564592374328No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)