FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488380

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488380
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences592866
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN57960.9776239487506451No Hit
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTACA29040.48982400744856347No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC13470.22720142494256712No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACGTAGCGCC12700.21421366716930976No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC12210.2059487304045096No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAAACT11510.19414167788336656No Hit
AGATAAGGGTATTAAACCTTATTATCCAGAACATGTAGTTAATCATTACT10230.17255163898756212No Hit
CCCTTATCTAATGGCAAATATTTGGTAACATTTGGATAAAATCTGGCAGG9850.16614209619037018No Hit
GTACAACATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC9230.15568442110021488No Hit
GTTTAATACCCTTATCTAATGGCAAATATTTGGTAACATTTGGATAAAAT9100.15349168277485975No Hit
ATACAAACCTTGCCAGCAAATCCGCCTCCTGCATCTACCAATCGTCAGTC8790.14826284522978211No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT8730.14725081215654126No Hit
GTATTAAACCTTATTATCCAGAACATGTAGTTAATCATTACTTCCAAACC8650.1459014347255535No Hit
CACTTACAGTCAATGAAAAACGAAGACTGCAATTAATTATGCCTGCCAGA8290.1398292362861085No Hit
GGATAATAAGGTTTAATACCCTTATCTAATGGCAAATATTTGGTAACATT8210.1384798588551207No Hit
CTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTT7880.13291367695229614No Hit
CTTACAGTCAATGAAAAACGAAGACTGCAATTAATTATGCCTGCCAGATT7560.12751616722834502No Hit
GTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTACATGTTCTGGAT7450.12566077326073682No Hit
ATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAG7370.12431139582974905No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACCTTCCACCAAACTCTGCAAG7170.12093795225227962No Hit
GTACCAAACCTTCGGACGGAAATTGCACCTGTATTCCCATCCCATCATCC7120.12009459135791224No Hit
GTCATAGAGTATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCAGC6920.1167211477804428No Hit
GTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTCTGTCTGATGTGAT6880.11604645906494891No Hit
TAATTATGCCTGCCAGATTTTATCCAAATGTTACCAAATATTTGCCATTA6750.11385372073959377No Hit
CATTTAAACCCAAACAAAACTAAACGCTGGGGTTACTCTTTAAATTTTAT6710.11317903202409987No Hit
ATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTCACGGTGGTCTCCATGC6700.11301035984522641No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT6600.11132363805649169No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCCTTGTATGCCTGTATT6370.10744417794240181No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTC6200.1045767509015528No Hit
TTATTATCCAGAACATGTAGTTAATCATTACTTCCAAACCAGACATTATT6120.10322737347056503No Hit
ATATAAGAGAGAAACAACACGTAGCGCCTCATTTTGTGGGTCACCATATT6000.10120330732408336No Hit
GGTTTGTATTATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGGCCTCCGT5960.10052861860858947No Hit
ACATTACAGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAAT5960.10052861860858947No Hit
GCCTAGCAGCCATGGAAATGATGTATATTTGCGGGAGAGAACAACAGAGT5930.10002260207196904No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)