FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002488381

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488381
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences592866
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN127962.158329200864951No Hit
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTACA25530.4306200726639746No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACGTAGCGCC12320.20780412437211784No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC11860.2000452041439381No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAAACT11090.18705744637068072No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC10990.185370724581946No Hit
AGATAAGGGTATTAAACCTTATTATCCAGAACATGTAGTTAATCATTACT10100.170358900662207No Hit
CCCTTATCTAATGGCAAATATTTGGTAACATTTGGATAAAATCTGGCAGG9780.16496139093825588No Hit
GTTTAATACCCTTATCTAATGGCAAATATTTGGTAACATTTGGATAAAAT9390.15838317596219043No Hit
GTACAACATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC9020.15214230534387196No Hit
GGATAATAAGGTTTAATACCCTTATCTAATGGCAAATATTTGGTAACATT8840.1491062061241495No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT8390.1415159580748432No Hit
GTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTACATGTTCTGGAT8260.13932321974948808No Hit
GTATTAAACCTTATTATCCAGAACATGTAGTTAATCATTACTTCCAAACC8220.1386485310339942No Hit
ATACAAACCTTGCCAGCAAATCCGCCTCCTGCATCTACCAATCGTCAGTC8010.13510641527765127No Hit
CTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTT7300.12313069057763475No Hit
ATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAG6930.11688981995931627No Hit
CACTTACAGTCAATGAAAAACGAAGACTGCAATTAATTATGCCTGCCAGA6890.11621513124382239No Hit
GTACCAAACCTTCGGACGGAAATTGCACCTGTATTCCCATCCCATCATCC6790.11452840945508766No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCCTTGTATGCCTGTATT6690.11284168766635294No Hit
CTTACAGTCAATGAAAAACGAAGACTGCAATTAATTATGCCTGCCAGATT6430.10845621101564266No Hit
GTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC6430.10845621101564266No Hit
GTCATAGAGTATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCAGC6360.10727550576352836No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACCACCGTG6310.106432144869161No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACCTTCCACCAAACTCTGCAAG6210.10474542308042625No Hit
GTTCTGGATAATAAGGTTTAATACCCTTATCTAATGGCAAATATTTGGTA5990.10103463514520988No Hit
GTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTCTGTCTGATGTGAT5990.10103463514520988No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)