FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488382

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488382
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences551755
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN26260.4759358773368615No Hit
CTTTTATATAAGATACCCGCCTTCCAAAGAGTGTGTAAATAATGTCTCGT21350.38694710514630587No Hit
TCCAAAGAGTGTGTAAATAATGTCTCGTTTGGAAGTAATGATTAACTACA16970.3075640456361972No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT15180.2751221103569519No Hit
GGATAATAAGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTGACATT14550.2637039990575527No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC13280.24068653659685912No Hit
ACTCACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT12220.22147511123596525No Hit
ATATGTTCCTGTGGCAATGTACCCCAACTCCCAATTACATATCCCATGAA11420.2069759222843472No Hit
TTATTATCCAGAGCATGTAGTTAATCATTACTTCCAAACGAGACATTATT11050.20027004739422388No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA10930.1980951690514812No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAGTTTTCTAGGGGGAACACCCGTG10920.19791392918958595No Hit
GGAACATATTGTACAAAAAATCAAAATGTGTTTTAGAAAACTTCCTGTAA10860.19682649001821462No Hit
CCCTTACAGTAAATGAAAAAAGGAGACTAAAATTAATTATGCCTGCTAGG10570.19157053402325308No Hit
GCACCGGACCATGCAAAACCTGCACAACTCCTGCTCAAGGAACCTCTATG9690.17562142617647325No Hit
CAATAGGCCTGTTTACAGGAAGTTTTCTAAAACACATTTTGATTTTTTGT9530.17272158838614965No Hit
CTACAGTACCTTGCTTTAATCCTAAATGGCAAACTCCTTCTTTCCCTGAC9340.16927803101014036No Hit
GTTTTATCCCAATGTCACTAAATATTTGCCCTTAGATAAAGGGATCAAAC8880.16094099736296003No Hit
CTCTTTTATATAAGATACCCGCCTTCCAAAGAGTGTGTAAATAATGTCTC8710.15785991971074118No Hit
GTTTACAGGAAGTTTTCTAAAACACATTTTGATTTTTTGTACAATATGTT8490.15387264274904625No Hit
GTGCTGGTGAACGCACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC8190.14843544689218946No Hit
GTCCGGTGCTGGTTGTTGATGATCCTGGAATTAGAGGACAAACGGGCAAC7980.14462940979238975No Hit
GTGTAAACGGGGCAGCAAACCCCAAAAGACCCACAATTCGTTGACATACT7920.1435419706210184No Hit
GTTGTGAGTATGCCCTGAGCCTGAGGGCTCCACCCCAACAGTCCCCCATG7890.1429982510353327No Hit
GTGTAAATAATGTCTCGTTTGGAAGTAATGATTAACTACATGCTCTGGAT7890.1429982510353327No Hit
AGTCTGTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTT7830.14191081186396137No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTCTCCCAAAAGTAAGA7660.13882973421174252No Hit
ATGTGTGGACTCTCTTTTATATAAGATACCCGCCTTCCAAAGAGTGTGTA7610.1379235349022664No Hit
ATATAAAAGAGAGTCCACACATAGCGCCTCATTTTGCGGGTCACCATATT7490.1357486565595237No Hit
GTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTTTTGTC7460.13520493697383804No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGTGTAAAC7310.13248633904540966No Hit
TCCTCAGGCCATGCAGTGGAACTCCACCACTTTCCACCAAACTCTTCAGG7260.13158013973593352No Hit
ATGTAATTGGGAGTTGGGGTACATTGCCACAGGAACATATTGTACAAAAA7010.1270491431885529No Hit
GCTCAGAATACTGTATCGGCCATATCGTCAATCTTATCGAAGACTGGGGA6950.12596170401718154No Hit
GCATTAAAGCAGGATATCCACATTGTGTAAACGGGGCAGCAAACCCCAAA6890.12487426484581018No Hit
ATTCTGAGCAGGGCTCACTGTTCCTGAACTGGAGCCACCAGCAGGAAAGT6890.12487426484581018No Hit
GAAAAAAGGAGACTAAAATTAATTATGCCTGCTAGGTTTTATCCCAATGT6780.12288062636496272No Hit
CCCTTATCTCCACCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAA6750.12233690677927703No Hit
GGGTAAAGGTTCAGATACTGTTTTCTGAGGAAGGTTTTGTAAATTGGCGA6710.12161194733169613No Hit
GTTGATAGATGTGAGCAATTTGTGGGGCCCCTTACAGTAAATGAAAAAAG6660.12070574802222No Hit
CCTTTATGCCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA6620.11998078857463912No Hit
ATATAAGATACCCGCCTTCCAAAGAGTGTGTAAATAATGTCTCGTTTGGA6600.11961830885084865No Hit
GTGTGTAAATAATGTCTCGTTTGGAAGTAATGATTAACTACATGCTCTGG6310.11436235285588713No Hit
TGATAGATGTGAGCAATTTGTGGGGCCCCTTACAGTAAATGAAAAAAGGA6260.113456153546411No Hit
CGTTAATATGGGCCTAAAACTCAGACAACTATTGTGGTTTCACATTTCCT6220.11273119409883009No Hit
ACAGTATTCTGAGCAGGGCTCACTGTTCCTGAACTGGAGCCACCAGCAGG6160.11164375492745875No Hit
CCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAACTCCACCACTTT6130.11110003534177307No Hit
CTCTTTGGAAGGCGGGTATCTTATATAAAAGAGAGTCCACACATAGCGCC6110.11073755561798262No Hit
GTGTGGACTCTCTTTTATATAAGATACCCGCCTTCCAAAGAGTGTGTAAA5990.10856267727523991No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGAAT5880.10656903879439243No Hit
TAGATAAAGGGATCAAACCTTATTATCCAGAGCATGTAGTTAATCATTAC5860.106206559070602No Hit
CAGTTATATGGATGATGTGGTTTTGGGGGCCAAGTCTGTACAACATCTTG5790.10493788003733541No Hit
CCCTTAACTTCATGGGATATGTAATTGGGAGTTGGGGTACATTGCCACAG5680.10294424155648793No Hit
GTTATATGGATGATGTGGTTTTGGGGGCCAAGTCTGTACAACATCTTGAG5630.10203804224701181No Hit
GCCTAGCAGCCATGGAAAGGATGTATACTTGCGGGAGAGCACGACAGAAT5610.10167556252322135No Hit
GGATATCCACATTGTGTAAACGGGGCAGCAAACCCCAAAAGACCCACAAT5590.10131308279943091No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)