FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002488383

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488383
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences551755
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN26070.4724923199608522No Hit
CTTTTATATAAGATACCCGCCTTCCAAAGAGTGTGTAAATAATGTCTCGT21180.38386602749408705No Hit
TCCAAAGAGTGTGTAAATAATGTCTCGTTTGGAAGTAATGATTAACTACA17060.3091952043932542No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT14710.2666038368478763No Hit
GGATAATAAGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTGACATT13930.25246712762004875No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC12210.22129387137407No Hit
ATATGTTCCTGTGGCAATGTACCCCAACTCCCAATTACATATCCCATGAA11260.20407608449402362No Hit
ACTCACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT11200.20298864532265226No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAGTTTTCTAGGGGGAACACCCGTG11180.20262616559886182No Hit
TTATTATCCAGAGCATGTAGTTAATCATTACTTCCAAACGAGACATTATT11020.1997263278085382No Hit
GGAACATATTGTACAAAAAATCAAAATGTGTTTTAGAAAACTTCCTGTAA10870.1970077298801098No Hit
CCCTTACAGTAAATGAAAAAAGGAGACTAAAATTAATTATGCCTGCTAGG10830.19628277043252895No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA9940.18015242272385387No Hit
GTTTACAGGAAGTTTTCTAAAACACATTTTGATTTTTTGTACAATATGTT9570.17344654783373054No Hit
GTTTTATCCCAATGTCACTAAATATTTGCCCTTAGATAAAGGGATCAAAC9550.1730840681099401No Hit
GCACCGGACCATGCAAAACCTGCACAACTCCTGCTCAAGGAACCTCTATG9520.17254034852425443No Hit
CTACAGTACCTTGCTTTAATCCTAAATGGCAAACTCCTTCTTTCCCTGAC9180.16637819321981676No Hit
AGTCTGTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTT9120.16529075404844543No Hit
CAATAGGCCTGTTTACAGGAAGTTTTCTAAAACACATTTTGATTTTTTGT8850.16039727777727433No Hit
GTGTAAATAATGTCTCGTTTGGAAGTAATGATTAACTACATGCTCTGGAT8370.15169776440630353No Hit
GTTGTGAGTATGCCCTGAGCCTGAGGGCTCCACCCCAACAGTCCCCCATG8210.14879792661597993No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTCTCCCAAAAGTAAGA8190.14843544689218946No Hit
GTCCGGTGCTGGTTGTTGATGATCCTGGAATTAGAGGACAAACGGGCAAC8070.14626056854944677No Hit
CTCTTTTATATAAGATACCCGCCTTCCAAAGAGTGTGTAAATAATGTCTC8040.14571684896376108No Hit
GTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTTTTGTC8040.14571684896376108No Hit
GTGCTGGTGAACGCACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC7850.14227329158775182No Hit
TCCTCAGGCCATGCAGTGGAACTCCACCACTTTCCACCAAACTCTTCAGG7590.13756105517847594No Hit
GTGTAAACGGGGCAGCAAACCCCAAAAGACCCACAATTCGTTGACATACT7450.13502369711194281No Hit
GCTCAGAATACTGTATCGGCCATATCGTCAATCTTATCGAAGACTGGGGA7440.1348424572500476No Hit
ATGTGTGGACTCTCTTTTATATAAGATACCCGCCTTCCAAAGAGTGTGTA7400.13411749780246668No Hit
ATATAAGATACCCGCCTTCCAAAGAGTGTGTAAATAATGTCTCGTTTGGA7380.1337550180786762No Hit
CCCTTATCTCCACCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAA7330.1328488187692001No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGTGTAAAC7150.12958650125508606No Hit
CTCTTTGGAAGGCGGGTATCTTATATAAAAGAGAGTCCACACATAGCGCC7050.1277741026361338No Hit
CCTTTATGCCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA6930.1255992242933911No Hit
ATATAAAAGAGAGTCCACACATAGCGCCTCATTTTGCGGGTCACCATATT6920.12541798443149588No Hit
ATGTAATTGGGAGTTGGGGTACATTGCCACAGGAACATATTGTACAAAAA6790.12306186622685793No Hit
GTGTGTAAATAATGTCTCGTTTGGAAGTAATGATTAACTACATGCTCTGG6560.11889334940326775No Hit
CGTTAATATGGGCCTAAAACTCAGACAACTATTGTGGTTTCACATTTCCT6500.1178059102318964No Hit
ATTCTGAGCAGGGCTCACTGTTCCTGAACTGGAGCCACCAGCAGGAAAGT6500.1178059102318964No Hit
GAAAAAAGGAGACTAAAATTAATTATGCCTGCTAGGTTTTATCCCAATGT6410.11617475147483937No Hit
GGGTAAAGGTTCAGATACTGTTTTCTGAGGAAGGTTTTGTAAATTGGCGA6190.11218747451314443No Hit
CCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAACTCCACCACTTT6070.11001259617040171No Hit
GTTGATAGATGTGAGCAATTTGTGGGGCCCCTTACAGTAAATGAAAAAAG5970.10820019755144947No Hit
GCTTTACTGCCTTTATATGCATGTATACAAGCAAAACAGGCTTTTACTTT5930.10747523810386857No Hit
CCTGTAAACAGGCCTATTGATTGGAAAGTATGTCAACGAATTGTGGGTCT5930.10747523810386857No Hit
ATATTTGCCCTTAGATAAAGGGATCAAACCTTATTATCCAGAGCATGTAG5820.10548159962302109No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT5750.10421292058975451No Hit
GCATTAAAGCAGGATATCCACATTGTGTAAACGGGGCAGCAAACCCCAAA5720.10366920100406884No Hit
ACTACATGCTCTGGATAATAAGGTTTGATCCCTTTATCTAAGGGCAAATA5670.10276300169459271No Hit
GTGTGGACTCTCTTTTATATAAGATACCCGCCTTCCAAAGAGTGTGTAAA5650.10240052197080225No Hit
ATATTAACGTTGACATAGCCGACTACTGCTTCCCTGGATGCTGGATCTTC5600.10149432266132613No Hit
CCCTTAACTTCATGGGATATGTAATTGGGAGTTGGGGTACATTGCCACAG5540.10040688348995479No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)