FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002488384

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488384
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences367186
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN52311.4246185856759246No Hit
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC13010.35431634103696763No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTTTCCCAAAAGTAAGA12270.3341630672193384No Hit
CTTTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGCGT10630.28949905497486284No Hit
TCCAAAGAGTGTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTACA10400.28323519959911325No Hit
CATTTATACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA10150.27642666114721154No Hit
CCCTTACAGTAAATGAAAACAGGAGACTAAAATTAATTATGCCTGCTAGG10110.2753372949949072No Hit
CGTTAATATGGGCCTAAAAATCAGACAATTATTGTGGTTTCATATTTCCT10060.27397558730452687No Hit
GGATAATATGGTTTGATCCCTTTATCTAATGGCAAATATTTAGTAACATT9540.2598138273245712No Hit
CCAAAAGACACCAAATATTCAAGAACAGTTTCTTTCCCAAAAGTAAGACA9310.25354997194882156No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA9110.24810314118730017No Hit
GGAACATATTGTACAAAAAATCAAAATGTGTTTTAGGAAACTTCCTGTAA9000.2451073842684634No Hit
AGTCTGTACAACATCTTGAGTCCATTTATACCGCTGTTACCAATTTTCTT8780.23911587043078986No Hit
GTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGTACAATATGTT8050.2192349381512367No Hit
GTACAACATCTTGAGTCCATTTATACCGCTGTTACCAATTTTCTTTTGTC6800.1851922458917279No Hit
ATATGTTCCTGTGGCAAAGTGCCCCAACTCCCAATTACATATCCCATGAA6710.18274117204904328No Hit
CTACATATTCTGGATAATATGGTTTGATCCCTTTATCTAATGGCAAATAT6170.16803472899293545No Hit
AGATAAAGGGATCAAACCATATTATCCAGAATATGTAGTTAATCATTACT6160.16776238745485939No Hit
ATTCTGGATAATATGGTTTGATCCCTTTATCTAATGGCAAATATTTAGTA6030.16422194745987048No Hit
CTGTTTACGTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAAAAGCCTGTT6020.1639496059217944No Hit
ATGGCAAATATTTAGTAACATTGGGATAAAACCTAGCAGGCATAATTAAT5870.15986448285065336No Hit
CAATAGGCCTGTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGT5690.15496233516528407No Hit
CTTCAAAGACTGTGTGTTTACTGAGTGGGAGGAGTTGGGGGAGGAGGTTA5410.1473367720991541No Hit
CCTTTATCTAATGGCAAATATTTAGTAACATTGGGATAAAACCTAGCAGG5390.14679208902300198No Hit
GCTTTATTCTTCTACGGTACCTTGCTTTAATCCTAAATGGCAAACTCCTT5300.14434101518031733No Hit
CATTTAAACCCTCACAAAACAAAACGATGGGGATATGCCCTTAACTTCAT5210.1418899413376327No Hit
GTTGATAGATGTAAGCAATTTGTGGGGCCCCTTACAGTAAATGAAAACAG5120.13943886749494808No Hit
ATATGGTTTGATCCCTTTATCTAATGGCAAATATTTAGTAACATTGGGAT5090.13862184288071985No Hit
GTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTACATATTCTGGAT5080.1383495013426438No Hit
CCCCCTGCATATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA4910.1337196951953506No Hit
TTCAAGAACAGTTTCTTTCCCAAAAGTAAGACAGGAAATATGAAACCACA4780.13017925520036167No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC4750.12936223058613347No Hit
GTATACATGCATATAAAGGCATCAAAGCAGGATATCCACATTGCGTGAAA4670.1271834982815249No Hit
GATGTAAGCAATTTGTGGGGCCCCTTACAGTAAATGAAAACAGGAGACTA4640.1263664736672967No Hit
GTGGATATCCTGCTTTGATGCCTTTATATGCATGTATACAAGCAAAACAG4610.12554944905306847No Hit
TCCAGGGACTTAGTAGTCAGCTATGTCAACGTTAATATGGGCCTAAAAAT4600.1252771075149924No Hit
ATATTAACGTTGACATAGCTGACTACTAAGTCCCTGGATGCTTGATCTTC4540.12364305828653599No Hit
GGGTAAAGGTTCAGATACTGTTTACGTAGAAAGGCCTTGTAAGTTGGCGA4530.1233707167484599No Hit
ATATTATCCAGAATATGTAGTTAATCATTACTTCCAGACGCGACATTATT4420.12037495982962314No Hit
GGATATCCACATTGCGTGAAAGGGGCAGCAAACCCCAAAAGACCCACAAT4370.11901325213924277No Hit
GGATAAAACCTAGCAGGCATAATTAATTTTAGTCTCCTGTTTTCATTTAC4350.11846856906309064No Hit
ATGTAATTGGGAGTTGGGGCACTTTGCCACAGGAACATATTGTACAAAAA4330.11792388598693851No Hit
GTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGC4310.11737920291078635No Hit
CCTGTAAACAGGCCTATTGATTGGAAAGTATGTCAACGAATTGTGGGTCT4290.11683451983463422No Hit
CATATAAAGGCATCAAAGCAGGATATCCACATTGCGTGAAAGGGGCAGCA4290.11683451983463422No Hit
TTGACATACTTTCCAATCAATAGGCCTGTTTACAGGAAGTTTCCTAAAAC4290.11683451983463422No Hit
ATTTCCCATTAGATAAAGGGATCAAACCATATTATCCAGAATATGTAGTT4130.1124770552254171No Hit
ACGTTAATATGGGCCTAAAAATCAGACAATTATTGTGGTTTCATATTTCC4100.11166003061118888No Hit
ACTTAGTAGTCAGCTATGTCAACGTTAATATGGGCCTAAAAATCAGACAA4080.11111534753503673No Hit
ACTCACAACTGTGCCAGCGGCTCCTCCTCCTGCCTCCACCAATCGGCAGT3940.10730256600197174No Hit
GGCAAATATTTAGTAACATTGGGATAAAACCTAGCAGGCATAATTAATTT3830.10430680908313497No Hit
CACCAATTTATGCCTACAGCCTCCTAGTACAAAGATCATTAACCTAACCT3790.10321744293083068No Hit
TCTATATGCAGGGGGAGTGCGAATCCACACTCCAAAAGACACCAAATATT3760.10240041831660249No Hit
GTGTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTACATATTCTGG3750.10212807677852642No Hit
CAGTAAATGAAAACAGGAGACTAAAATTAATTATGCCTGCTAGGTTTTAT3710.10103871062622215No Hit
CCCCAAAAGACCCACAATTCGTTGACATACTTTCCAATCAATAGGCCTGT3690.10049402755006999No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)