FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488386

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488386
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences543394
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN42830.7881942016290207No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG32820.6039816413136693No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG29440.5417799975708234No Hit
CTTATATAGAATGCCAGCCTTCCACAGGGTATGTAAATAATGCCTAGTTT25370.46688038513491126No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA23200.4269461937378771No Hit
CTCTAGAACAGTTTCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACA21940.40375859873314757No Hit
GTTTAGGATAAAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA17740.32646661538404914No Hit
TTCACTTACAGGAGGACATTATTAATAGATGTCAACAATATGTGGGCCCT17640.32462633006621344No Hit
GGGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCA17140.31542490347703506No Hit
ATCCTAAACTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCCTAT16650.30640750541964024No Hit
GGATAATAGGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTAAGTTT15350.2824837962877765No Hit
CTTACAGGAGGACATTATTAATAGATGTCAACAATATGTGGGCCCTCTTA14840.27309834116681453No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGGGTATGTAAATAATGCCTAGT13880.255431602115592No Hit
CAATACTAATATGGGCCTAAAACTCAGACAACTATTGTGGTTTCACATTT13590.25009477469386854No Hit
ATGTTAATATGGGCCTAAAACTCAGACAACTATTGTGGTTTCACATTTCC12570.23132386445194464No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC12320.22672315115735545No Hit
CTCGAGAACAGTTTCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACA12000.22083423814028127No Hit
ATTCTTTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAGTTTCCG10640.19580635781771605No Hit
TCCACAGGGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCA10290.18936535920529118No Hit
GTTCAGGATAATAGGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTA9810.18053198967967996No Hit
GTTTTGAAGTAATGATTAACTGCATGTTCAGGATAATAGGGTTTAATGCC9530.17537919078974004No Hit
CTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGGTT9450.1739069625354715No Hit
ACTCTAGAACAGTTTCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCAC9280.17077847749515085No Hit
ATACTAATATGGGCCTAAAACTCAGACAACTATTGTGGTTTCACATTTCC9250.17022639189980016No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT8940.16452150741450955No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCTCAG8860.163049279160241No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC8710.16028885118348749No Hit
CAATGTTAATATGGGCCTAAAACTCAGACAACTATTGTGGTTTCACATTT8380.15421590963462975No Hit
TTCATTTACAGGAGGACATTATTAATAGATGTCAACAATATGTGGGCCCT7630.14041376975086217No Hit
CTATTATCCTGAACATGCAGTTAATCATTACTTCAAAACTAGGCATTATT7420.13654917058340726No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG7370.13562902792448941No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATACCCACATTGTGTAAAA7310.134524856733788No Hit
ATATGAAGCGCTGCGTGAAGTTTCTCTCTTATATAGAATGCCAGCCTTCC7090.13047622903454953No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCCTCCTGT7040.12955608637563168No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT6980.12845191518493027No Hit
CTCCAAAAGACACCAAATACTCTAGAACAGTTTCTCTTCCAAAAGTAAGA6910.1271637154624453No Hit
TATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAAA6890.12679565839887816No Hit
TACCCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCT6820.12550745867639318No Hit
AATATGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTAAGTTTAGGA6730.12385120189034107No Hit
CCTTTATCCAAGGGCAAATATTTGGTAAGTTTAGGATAAAACCTAGCAGG6610.12164285950893826No Hit
CTACTGTACCTGTCTTTAATCCGGAGTGGCAAACTCCCTCCTTTCCTAAC6480.11925048859575188No Hit
GGGTAAAGGTGCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGACGA6480.11925048859575188No Hit
GTTTAATGCCTTTATCCAAGGGCAAATATTTGGTAAGTTTAGGATAAAAC6430.11833034593683403No Hit
CTGTAATGTCAACGACCGACCTTGAGGCATACTTCAAAGACTGTTTGTTT6410.1179622888732669No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCTGTAATGTCAACGACCGACCT6290.11575394649186412No Hit
TTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAGTTTCCGAAAAC6230.1146497753011627No Hit
ATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAAAG6190.11391366117402843No Hit
GGAACATATTGTACAAAAACTCAAGCAATGTTTTCGGAAACTGCCTGTAA6190.11391366117402843No Hit
CTATATAAGAGAGAAACTTCACGCAGCGCTTCATATTGTGGGTCACCATA6170.1135456041104613No Hit
ATGTGGTATTGGGGGCGAAGTCTGTACAACATCTTGAGTCCCTTTTTACC6160.11336157557867772No Hit
ATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGGAT6140.1129935185151106No Hit
ATATGTTCCTGAGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA6100.11225740438797632No Hit
GTACAACATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC6020.11078517613370778No Hit
GATTAACTGCATGTTCAGGATAATAGGGTTTAATGCCTTTATCCAAGGGC5890.1083928052205214No Hit
CAATATGTGGGCCCTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAAT5880.10820877668873782No Hit
GTTTTATCCTAAACTTACCAAATATTTGCCCTTGGATAAAGGCATTAAAC5820.10710460549803642No Hit
TTCTTCTACTGTACCTGTCTTTAATCCGGAGTGGCAAACTCCCTCCTTTC5790.10655251990268572No Hit
AAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGGATAATA5740.10563237724376788No Hit
ATCCTGAACATGCAGTTAATCATTACTTCAAAACTAGGCATTATTTACAT5710.10508029164841717No Hit
GTCTATTTACAGGCAGTTTCCGAAAACATTGCTTGAGTTTTTGTACAATA5670.1043441775212829No Hit
GGATAAAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCATTAAC5660.10416014898949934No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTC5610.1032400063305815No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCGAAGTCTGTACAACATCTTG5560.10231986367166365No Hit
GAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGGTTTTATCCTAAACT5520.10158374954452938No Hit
ATTTACAGGCAGTTTCCGAAAACATTGCTTGAGTTTTTGTACAATATGTT5480.10084763541739511No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)