FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488387

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488387
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences543394
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN42390.7800969462305436No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG34940.6429956900517856No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG28300.5208007449474966No Hit
CTTATATAGAATGCCAGCCTTCCACAGGGTATGTAAATAATGCCTAGTTT23590.4341233064774363No Hit
CTCTAGAACAGTTTCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACA22450.41314405385410957No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA20710.38112308932376876No Hit
GTTTAGGATAAAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA19790.36419246439968056No Hit
GGGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCA16860.31027210458709514No Hit
ATCCTAAACTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCCTAT16730.3078797336739088No Hit
TTCACTTACAGGAGGACATTATTAATAGATGTCAACAATATGTGGGCCCT14950.27512265501643374No Hit
GGATAATAGGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTAAGTTT14500.26684137108617323No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGGGTATGTAAATAATGCCTAGT13220.24328571901787652No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC12240.2252509229030869No Hit
CAATACTAATATGGGCCTAAAACTCAGACAACTATTGTGGTTTCACATTT12240.2252509229030869No Hit
CTTACAGGAGGACATTATTAATAGATGTCAACAATATGTGGGCCCTCTTA11650.2143932395278564No Hit
ATGTTAATATGGGCCTAAAACTCAGACAACTATTGTGGTTTCACATTTCC11560.2127369827418043No Hit
ATTCTTTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAGTTTCCG10400.19138967305491042No Hit
CTCGAGAACAGTTTCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACA10330.19010147333242544No Hit
GTTTTGAAGTAATGATTAACTGCATGTTCAGGATAATAGGGTTTAATGCC10250.1886292450781569No Hit
GTTCAGGATAATAGGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTA9880.18182018940216493No Hit
ATACTAATATGGGCCTAAAACTCAGACAACTATTGTGGTTTCACATTTCC9230.169858334836233No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT9070.1669138783276959No Hit
CTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGGTT9000.16562567860521096No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCTCAG8650.15918467999278607No Hit
TCCACAGGGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCA8520.1567923090795997No Hit
ACTCTAGAACAGTTTCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCAC8350.15366382403927903No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC7710.14188599800513071No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG7230.13305262847951946No Hit
CTATTATCCTGAACATGCAGTTAATCATTACTTCAAAACTAGGCATTATT6970.1282678866531467No Hit
CAATGTTAATATGGGCCTAAAACTCAGACAACTATTGTGGTTTCACATTT6890.12679565839887816No Hit
TATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAAA6870.12642760133531103No Hit
TTCATTTACAGGAGGACATTATTAATAGATGTCAACAATATGTGGGCCCT6520.11998660272288615No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATACCCACATTGTGTAAAA6520.11998660272288615No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCCTCCTGT6480.11925048859575188No Hit
ATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAAAG6440.11851437446861761No Hit
ATATGAAGCGCTGCGTGAAGTTTCTCTCTTATATAGAATGCCAGCCTTCC6430.11833034593683403No Hit
GTTTAATGCCTTTATCCAAGGGCAAATATTTGGTAAGTTTAGGATAAAAC6360.11704214621434907No Hit
CTCCAAAAGACACCAAATACTCTAGAACAGTTTCTCTTCCAAAAGTAAGA6290.11575394649186412No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT6290.11575394649186412No Hit
GTCTATTTACAGGCAGTTTCCGAAAACATTGCTTGAGTTTTTGTACAATA6250.11501783236472984No Hit
CCTTTATCCAAGGGCAAATATTTGGTAAGTTTAGGATAAAACCTAGCAGG6090.11207337585619276No Hit
GTACAACATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC6050.11133726172905849No Hit
ATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGGAT5960.10968100494300637No Hit
GTTTTATCCTAAACTTACCAAATATTTGCCCTTGGATAAAGGCATTAAAC5940.10931294787943924No Hit
ATATGTTCCTGAGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA5850.10765669109338713No Hit
CTATATAAGAGAGAAACTTCACGCAGCGCTTCATATTGTGGGTCACCATA5840.10747266256160355No Hit
TACCCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCT5750.10581640577555144No Hit
GGATAAAGGCATTAAACCCTATTATCCTGAACATGCAGTTAATCATTACT5720.10526432018020074No Hit
AATATGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTAAGTTTAGGA5640.1037920919259322No Hit
TTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAGTTTCCGAAAAC5640.1037920919259322No Hit
GGGTAAAGGTGCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGACGA5640.1037920919259322No Hit
GATTAACTGCATGTTCAGGATAATAGGGTTTAATGCCTTTATCCAAGGGC5620.10342403486236507No Hit
CTACTGTACCTGTCTTTAATCCGGAGTGGCAAACTCCCTCCTTTCCTAAC5610.1032400063305815No Hit
ATGTGGTATTGGGGGCGAAGTCTGTACAACATCTTGAGTCCCTTTTTACC5580.1026879207352308No Hit
CTTTTACACAATGTGGGTATCCTGCCTTGATGCCTTTATATGCATGTATA5490.10103166394917869No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCTGTAATGTCAACGACCGACCT5490.10103166394917869No Hit
CTATTAATAATGTCCTCCTGTAAGTGAATGTTAGGAAAGGAGGGAGTTTG5480.10084763541739511No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)