FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002488390

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488390
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences440485
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTTATAGGAAGTTTCCGAAAACATTGTTTGATTTTTTGTACAATATGTT25590.5809505431512991No Hit
CTACTGTACCTGTCTTTAACCCTGAATGGAAAACTCCCTCTTTTCCTCAC20920.47493104191970215No Hit
ATTTATGGGAAGTTTCCGAAAACATTGTTTGATTTTTTGTACAATATGTT19620.44541811866465375No Hit
GGATATTATCAATAGATGTCAACAATATGTAGGCCCTCTTACAGTTAATG19300.4381533990941803No Hit
ATTTGTAGGAAGTTTCCGAAAACATTGTTTGATTTTTTGTACAATATGTT17140.38911654199348444No Hit
CCTATCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA15010.3407607523525205No Hit
GGTTAGGATAGAACCTAGCAGGCATGATCAATTTTAATCTCCGTTTTTCA14510.32940962802365575No Hit
CCTATTCTCCTCCCCCAACTCCTCCCAGTCTTTAAATACACAGTCTTTGA13980.3173774362350591No Hit
GCTTAGCTTGTATACATGCATACAAAGGCATTAATGCTGGGTAACCACAT11450.25994074713100335No Hit
CCAAAAGACACCAAATACTCAAGAATAGTTTCTCTTCCAAAAGTAAGACA11360.25789754475180765No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA11300.2565354098323439No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN10910.24768153285582936No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAGATC10860.2465464204229429No Hit
CTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAACCCTAATAAA10850.2463193979363656No Hit
CTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACCTTGCCACAGGA10790.24495726301690182No Hit
AGTCTGTACAACATCTTGAATCCCTTTTTACCGCTATTACCAATTTTCTT10120.22974675641622305No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAA10000.2270224865772955No Hit
GGATAGAACCTAGCAGGCATGATCAATTTTAATCTCCGTTTTTCATTAAC9930.22543332917125442No Hit
ATATGTTCCTGTGGCAAGGTACCCCAACTTCCAATTACATATCCCATGAA9820.22293608181890417No Hit
CTCCAAAAGACACCAAATACTCAAGAATAGTTTCTCTTCCAAAAGTAAGA9670.21953074452024474No Hit
CTATAACCAATTTTCTTTTGTCTTTGGGTATACATTTAAACCCTAATAAA9600.21794158711420367No Hit
CAACTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGAAGAGAAACTAT9230.20954175511084372No Hit
GTCTTTAAATACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA9110.20681748527191618No Hit
GGTACGGTTAGGATAGAACCTAGCAGGCATGATCAATTTTAATCTCCGTT8800.19977978818802003No Hit
CCTACAAATAGACCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGTCT8570.19455827099674222No Hit
GTATACATGCATACAAAGGCATTAATGCTGGGTAACCACATTGGGTAAAG8320.18888270883230984No Hit
CATTTAAACCCTAATAAAACCAAACGTTGGGGTTACTCCCTTAACTTCAT8230.18683950645311417No Hit
GTTAGTATCCCTTGGACTCATAAGGTGGGAAATTTTACTGGGCTTTATTC7860.17843967444975425No Hit
CTCTTACAGTTAATGAAAAACGGAGATTAAAATTGATCATGCCTGCTAGG7830.17775860699002236No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG7780.17662349455713588No Hit
GTACCTGTCTTTAACCCTGAATGGAAAACTCCCTCTTTTCCTCACATTCA7560.17162899985243538No Hit
TTCTTCTACTGTACCTGTCTTTAACCCTGAATGGAAAACTCCCTCTTTTC7560.17162899985243538No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT7390.16776961758062137No Hit
CTCATAAGGTGGGAAATTTTACTGGGCTTTATTCTTCTACTGTACCTGTC7320.1661804601745803No Hit
CTGTAAGAGGGCCTACATATTGTTGACATCTATTGATAATATCCTCTTTC7180.16300214536249816No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACCTTGCCACAG7170.16277512287592086No Hit
GTTACATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAA6750.15324017843967444No Hit
GTCTTTAACCCTGAATGGAAAACTCCCTCTTTTCCTCACATTCATTTGAA6730.15278613346651984No Hit
ATGTAAGACCTTGGGCAAGACCTGGCGGGCGTTCACGGTGGTCTCCATGC6690.15187804352021067No Hit
GGGTAAAGGGAGCGGCAAAGCCCAGAAGACCCACAATTCTTTGACATACT6610.15006186362759233No Hit
CAATGTTAGTATCCCTTGGACTCATAAGGTGGGAAATTTTACTGGGCTTT6600.149834841141015No Hit
GTACAACATCTTGAATCCCTTTTTACCGCTATTACCAATTTTCTTTTGTC6530.14824568373497396No Hit
ACAATATGTAGGCCCTCTTACAGTTAATGAAAAACGGAGATTAAAATTGA6460.1466565263289329No Hit
GCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAACCCTAATAA6420.1457484363826237No Hit
CCCTTTACCCAATGTGGTTACCCAGCATTAATGCCTTTGTATGCATGTAT6400.14529439140946912No Hit
GTTCAGGATAATAAGGTTTAATGCCTTTATCTAAAGGTAAATATTTGGTA6370.14461332394973722No Hit
TTATAGGAAGTTTCCGAAAACATTGTTTGATTTTTTGTACAATATGTTCC6310.14325118903027345No Hit
CAATAGATGTCAACAATATGTAGGCCCTCTTACAGTTAATGAAAAACGGA6280.14257012157054158No Hit
ATGTCAATATGGGCCTAAAACTCAGACAAATATTGTGGTTTCACATTTCC6250.14188905411080968No Hit
GCTTTATTCTTCTACTGTACCTGTCTTTAACCCTGAATGGAAAACTCCCT6210.14098096416450048No Hit
GAAATTAAGGGAGTAACCCCAACGTTTGGTTTTATTAGGGTTTAAATGTA6140.1393918067584594No Hit
CCCTTGGACTCATAAGGTGGGAAATTTTACTGGGCTTTATTCTTCTACTG6090.13825669432557294No Hit
CTCCTATCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCG6060.13757562686584104No Hit
GGTTCCTTGAGCAGGAATCGTGCAGGTCTTGCATGGTCCCGTGCTGGTAG5940.1348513570269135No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAATCCCTTTTTACC5800.1316730422148314No Hit
CATAAAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGCTTAGCTTG5700.12940281734905842No Hit
GTATTGGGGGCCAAGTCTGTACAACATCTTGAATCCCTTTTTACCGCTAT5690.12917579486248112No Hit
CCTATCGCTTACAGACCACCAAATACTCAAGAATAGTTTCTCTTCCAAAA5590.12690556999670818No Hit
GTTTCGCTCCAGACCGGCTGCGAGCAAAACAAGCTGCTAGGAGTTCCGCA5510.12508939010408981No Hit
CTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTTGTTT5500.12486236761751252No Hit
ACATATTGTTGACATCTATTGATAATATCCTCTTTCAAATGAATGTGAGG5460.12395427767120334No Hit
ATCTAAAGGTAAATATTTGGTACGGTTAGGATAGAACCTAGCAGGCATGA5460.12395427767120334No Hit
CCCATAAATAGACCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGTCT5450.12372725518462603No Hit
GCTGGTATTTTATATAAGAGAGAAACTACTCGCAGCGCCTCATTTTGTGG5400.12259214275173956No Hit
ATGTAGGCCCTCTTACAGTTAATGAAAAACGGAGATTAAAATTGATCATG5360.12168405280543038No Hit
GGTAAATATTTGGTACGGTTAGGATAGAACCTAGCAGGCATGATCAATTT5340.12123000783227579No Hit
TATTAACATTGACATAGCTTACTACTAATTCCCTGGATGCTGGGTCTTCC5300.12032191788596659No Hit
CATCTATTGATAATATCCTCTTTCAAATGAATGTGAGGAAAAGAGGGAGT5280.11986787291281202No Hit
TATCAATAGATGTCAACAATATGTAGGCCCTCTTACAGTTAATGAAAAAC5270.11964085042623471No Hit
GTATGTAAATAATGCCTTGTTTGGAAGTAATGATTAACTATATGTTCAGG5260.11941382793965742No Hit
CCCTTAATTTCATGGGATATGTAATTGGAAGTTGGGGTACCTTGCCACAG5250.11918680545308014No Hit
CTATATGTTCAGGATAATAAGGTTTAATGCCTTTATCTAAAGGTAAATAT5220.11850573799334825No Hit
CAGTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTG5100.1157814681544207No Hit
ATTTTAATCTCCGTTTTTCATTAACTGTAAGAGGGCCTACATATTGTTGA5060.11487337820811153No Hit
CCTTTACCCCGTTGCTCGGCAACGGTCAGGTCTTTGCCAAGTGTTTGCTG4960.11260315334233856No Hit
GGTCCCCTAGAAGAAGAACTCCCTCGCCTCGCAGACGAAGGTCTCAATCG4900.11124101842287477No Hit
ATCTAAAGGTAAATATTTGGTAAGGTTAGGATAGAACCTAGCAGGCATGA4830.10965186101683372No Hit
AGTTAAGGGAGTAACCCCAACGTTTGGTTTTATTAGGGTTTAAATGTATA4810.10919781604367912No Hit
GCTATAACCAATTTTCTTTTGTCTTTGGGTATACATTTAAACCCTAATAA4800.10897079355710183No Hit
GGATAATAAGGTTTAATGCCTTTATCTAAAGGTAAATATTTGGTAAGGTT4780.10851674858394723No Hit
CCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAA4750.10783568112421535No Hit
ATCCAAAGGTAAATATTTGGTACGGTTAGGATAGAACCTAGCAGGCATGA4720.10715461366448346No Hit
AGGATATTATCAATAGATGTCAACAATATGTAGGCCCTCTTACAGTTAAT4690.10647354620475158No Hit
TCCCATAAATAGACCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGTC4680.10624652371817428No Hit
GGTTTAATGCCTTTATCTAAAGGTAAATATTTGGTAAGGTTAGGATAGAA4630.1051114112852878No Hit
CGGGAATCTCAATGTTAGTATCCCTTGGACTCATAAGGTGGGAAATTTTA4620.10488438879871051No Hit
CAAAACACCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGG4580.10397629885240134No Hit
CAAGGAACCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGG4540.10306820890609214No Hit
GGTAAATATTTGGTAAGGTTAGGATAGAACCTAGCAGGCATGATCAATTT4540.10306820890609214No Hit
GCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT4530.10284118641951485No Hit
CCTATAAATAGACCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGTCT4500.10216011895978297No Hit
CTTGAATCCCTTTTTACCGCTATTACCAATTTTCTTTTGTCTTTGGGTAT4500.10216011895978297No Hit
GTACAATATGTTCCTGTGGCAAGGTACCCCAACTTCCAATTACATATCCC4460.10125202901347378No Hit
GTTTAATGCCTTTATCTAAAGGTAAATATTTGGTACGGTTAGGATAGAAC4450.10102500652689649No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)