FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488392

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488392
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences521227
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATAGAGTGTGTAAATAATGTCTGGTCTGGAAGTAATGATTAACTAGA25300.4853931204638286No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAATACAAAAATC18950.3635652028770574No Hit
CTCTAAGACCGTGTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA18740.3595362481222193No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN18720.359152538145568No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC16850.3232756553286764No Hit
GTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATTTGGGTAAAAC15210.291811437243274No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG14560.27934086300210853No Hit
CATTTACACCAAGACATCATCAAAAAATGTGAACAATTTGTAGGCCCACT12650.2426965602319143No Hit
GTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACTTCCAGACC11180.21449387694804758No Hit
TAATACACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTCTGGAAGT10670.2047092725434408No Hit
GTCTTAGAGTATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCAGC10150.19473281315050833No Hit
CCCTTATCCAATGGCAAATATTTGGTAACATTTGGGTAAAACCTAGCAGG9960.19108756837232146No Hit
CTACCGTTCCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTCCTAAG9770.18744232359413462No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACATAGCGCC9690.1859074836875296No Hit
TTGTCAGGGTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGC9550.18322151385097088No Hit
CTACAAACCTTGCCAGCAAATCCGCCTCCTGCCTCCACCAATCGCCAGTC9180.1761228792829228No Hit
GGATAAGGGTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACT9060.17382061942301533No Hit
GTGTAAAAGGGGCAGCAAACCCCAAAAGACCCACAATACGTTGACAGACT9020.17305319946971282No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACGCTTCCGGA8720.16729754981994407No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT8620.16537899993668784No Hit
ATGTAAGACCTTGGGCAAGAAGTGGTGGGCGTTCACGGTGGTCTCCATGC8380.16077448021687288No Hit
CACTAATATGGGCCTAAAGTTCAGGCAACTATTGTGGTTTCACATTTCTT8230.15789665539198852No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGAGAGAACAACAGAGT7780.14926318091733543No Hit
GTATCAAACCTTATTATCCAGAACATCTAGTTAATCATTACTTCCAGACC7690.14753648602240482No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAAGTGGTGGGCGTTC7530.14446680620919483No Hit
ATATAGAGCTGAGGCGGTATCTAGAAGATCTCGTACTGAAGGAAAGAAGT7410.14216454634928735No Hit
TAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTCTGGAAGT7410.14216454634928735No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC7300.14005414147770548No Hit
ACATTACAGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAAG7240.13890301154775173No Hit
CTCACAGTCAATGAGAAAAGAAGACTGCAATTGATTATGCCTGCTAGGTT7190.13794373660612363No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA7120.13660075168784427No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCCTTGTATGCATGTATT7000.13429849182793677No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCAGTACG6910.13257179693300616No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT6900.13237994194468053No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAA6880.1319962319680293No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC6870.13180437697970365No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG6810.13065324704974993No Hit
ATATTAGTGTTGACATAACTGACTACTAGGTCCCTGGATGTTGGATCTTC6650.12758356723653994No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA6650.12758356723653994No Hit
GGTTTGTAGTATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGGCCTCCGT6510.1248975973999812No Hit
GTACAACATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC6510.1248975973999812No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC6490.12451388742332994No Hit
CTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTCT6460.12393832245835307No Hit
GTCCCGTGCTGGTGGTTGACGATCCTGGAATTAGAGGACAAACGGGCAAC6330.12144420761011995No Hit
CACCAAGACATCATCAAAAAATGTGAACAATTTGTAGGCCCACTCACAGT6320.12125235262179435No Hit
ATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTCTGGA6310.12106049763346872No Hit
GCTTTATTCTTCTACCGTTCCTGTCTTTAACCCTCATTGGAAAACACCCT6250.11990936770351497No Hit
GTATCTCTTGTTCCCAAGAATATGGTGACCCACAGAATGAGGCGCTATGT6240.11971751271518936No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCGTCAACCACC6230.11952565772686372No Hit
TCCTTGGACTCATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACCG6210.11914194775021249No Hit
GTTTGATACCCTTATCCAATGGCAAATATTTGGTAACATTTGGGTAAAAC6160.11818267280858435No Hit
GTGTAAATGTATCTTAGGAAAAGAGGGTGTTTTCCAATGAGGGTTAAAGA6150.11799081782025873No Hit
CTCATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACCGTTCCTGTC6090.116839687890305No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA6070.11645597791365375No Hit
ATTTATGCCTACAGCCTCCTAATACAAAAATCATTAATCTAGTCTCCTCC6070.11645597791365375No Hit
GCTTTAGGGCATGGACATTGATCCTTATAAAGAATTTGGAGCTACTGTGG6060.11626412292532812No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT6010.1153048479837No Hit
CCTGTATTCCCATCCCATCATCTTGGGCTTTCGGAAAATTCCTATGGGAG6010.1153048479837No Hit
TCCACCAAACTCTGCAAGATCCCAGAGTGAAAGGCCTGTATTTCCCTGCT6000.11511299299537439No Hit
ATCTAGAAGATCTCGTACTGAAGGAAAGAAGTCAGAAGGCAAAAACGAGA5890.1130025881237925No Hit
CTTTAACCCTCATTGGAAAACACCCTCTTTTCCTAAGATACATTTACACC5890.1130025881237925No Hit
GCACGGGACCATGCAGAACCTGCACGACTCCTGCTCAAGGAACCTCTATG5880.11281073313546688No Hit
ACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCTGCATGGTCCCGTG5870.11261887814714128No Hit
CTCTAAGGCTTCTCGATATAGAGCTGAGGCGGTATCTAGAAGATCTCGTA5790.11108403824053627No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAG5750.11031661828723377No Hit
GGTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATTTGGGTAAAA5740.11012476329890816No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG5650.10839806840397753No Hit
GTGTAAATAATGTCTGGTCTGGAAGTAATGATTAACTAGATGTTCTGGAT5600.10743879346234943No Hit
GGATAATAAGGTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATT5580.10705508348569817No Hit
GCCTTAGAGTCTCCTGAGCATTGTTCACCTCACCATACTGCACTCAGGCA5570.10686322849737255No Hit
GTCAATGAGAAAAGAAGACTGCAATTGATTATGCCTGCTAGGTTTTACCC5470.10494467861411631No Hit
GTATGGATCGGCAGCGGAGCCGGAAAGGTTCCACGCATGCGCTGATGGCC5440.10436911364913944No Hit
GCCTGAGGATGAGTATTTCTCAAAGGTGGAGACAGAGGGGTAGGCTGCCT5440.10436911364913944No Hit
GCTCTATATCGAGAAGCCTTAGAGTCTCCTGAGCATTGTTCACCTCACCA5380.1032179837191857No Hit
CAATAGGCCGGTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTCTGT5370.10302612873086008No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAG5360.10283427374253443No Hit
GTGCACACGGTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAA5330.10225870877755758No Hit
GAAAAGAAGACTGCAATTGATTATGCCTGCTAGGTTTTACCCAAATGTTA5320.10206685378923194No Hit
TTATGGGTCCTTGCCACAAGATCACATCATACAGAAAATCAAAGAATGTT5280.10129943383592944No Hit
GACCTGGCCGTTGCCGGGCAACGGGGTAAAGGTTCAGGTATTGTTTACAC5280.10129943383592944No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCTGTAATGTCAACGACCGACCT5260.10091572385927819No Hit
AGCTGGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATACTCTAAGA5250.10072386887095258No Hit
CCTCTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGC5240.10053201388262696No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)