FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002488394

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488394
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences425756
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTAAACTTAGTCATGCCTGCTAGATTTTTTCCTATCTCTACGAAATAT30750.722244665958906No Hit
CTACTATACCTGTCTTTAATCCTAACTGGAAAACTCCATCTTTTCCTGAT26650.6259453771643853No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN24180.5679309275735398No Hit
TCCATAGGGTATGTAAATAGTGTCTGGTTTGGAAGTAATGATTAACCACA23900.5613543907778163No Hit
ACTATACCTGTCTTTAATCCTAACTGGAAAACTCCATCTTTTCCTGATAT17450.4098591681620458No Hit
ATGTAAATAGTGTCTGGTTTGGAAGTAATGATTAACCACATTATCCGGAT16900.3969409708847321No Hit
GTTTTATACCTTTCTCTAGGGGCAAATATTTCGTAGAGATAGGAAAAAAT14260.334933623953626No Hit
AGTTTAATCTTCGTTTTTCATTTACTGTTAGAGGACCTACAAATTGTTCA13550.3182574056501846No Hit
GGTTGAACTTAGTCATGCCTGCTAGATTTTTTCCTATCTCTACGAAATAT12050.283025958530238No Hit
TGTAAATAGTGTCTGGTTTGGAAGTAATGATTAACCACATTATCCGGATA11880.27903306118997734No Hit
AGTTCAACCTTCGTTTTTCATTTACTGTTAGAGGACCTACAAATTGTTCA11690.2745704112214508No Hit
TTCTTGGACTGTCCCTCTCGAATGGGGGAAGAATCATTCCACCACCAATC11100.2607127086876051No Hit
GTCCAAGAAAGCCCCATGATGTAGCTCTTGTTCCCAAGAATAAGGTGACC10630.24967352192335518No Hit
CTCTTCTACTATACCTGTCTTTAATCCTAACTGGAAAACTCCATCTTTTC10550.24779451141029135No Hit
GTTCAACCTTCGTTTTTCATTTACTGTTAGAGGACCTACAAATTGTTCAC10210.2398087167297701No Hit
CTTTAATCCTAACTGGAAAACTCCATCTTTTCCTGATATTCATTTGCACC9700.22783002470898825No Hit
CTTTATACCTCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAA9570.22477663262525951No Hit
GTATGTAAATAGTGTCTGGTTTGGAAGTAATGATTAACCACATTATCCGG9010.2116235590338128No Hit
CTTCTACTATACCTGTCTTTAATCCTAACTGGAAAACTCCATCTTTTCCT8910.209274795892483No Hit
TCCAAAGGGTATGTAAATAGTGTCTGGTTTGGAAGTAATGATTAACCACA8350.19612172230103628No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC8130.19095444339011078No Hit
CAATCTCCAGCTTCCCAATGTTAGTATTCCTTGGACTCACAAGGTGGGAA7940.18649179342158417No Hit
CCTACAAATTGTTCACATTTGTTAATAATGTCCTGGTGCAAATGAATATC7850.18437790659438738No Hit
CCTTTATACCTCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA7830.18390815396612142No Hit
GCTTTACTCTTCTACTATACCTGTCTTTAATCCTAACTGGAAAACTCCAT7780.18273377239545657No Hit
CTGTTAGAGGACCTACAAATTGTTCACATTTGTTAATAATGTCCTGGTGC7710.1810896381965257No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCTCCCGTG7390.17357359614427043No Hit
ACATTATCCGGATAATAAGGTTTTATACCTTTCTCTAGGGGCAAATATTT7360.1728689672018715No Hit
CTCTAACAGTAAATGAAAAACGAAGATTAAACTTAGTCATGCCTGCTAGA7070.16605755409201514No Hit
GGGTATGTAAATAGTGTCTGGTTTGGAAGTAATGATTAACCACATTATCC7050.16558780146374918No Hit
GTGTAAAAGGGGCAGCAAAACCCAAGAGACCCACAATTCTTTGACATACT6990.1641785435789513No Hit
ACTGAGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCAGTTAG6890.16182978043762156No Hit
CTATTTACATACCCTATGGAAGGCGGGCATCTTATATAAAAGAGAAACTA6870.1613600278093556No Hit
ATTTGTAGGTCCTCTAACAGTAAATGAAAAACGAAGATTAAACTTAGTCA6870.1613600278093556No Hit
TAATAAGGTTTTATACCTTTCTCTAGGGGCAAATATTTCGTAGAGATAGG6790.15948101729629177No Hit
TTATTAACAAATGTGAACAATTTGTAGGTCCTCTAACAGTAAATGAAAAA6550.15384398575710032No Hit
TTACATAACCCATGAAATTTAGGGAATATCCCCATCTTTTTGTTTTGTTG6390.15008596473097266No Hit
CCCTATGGAAGGCGGGCATCTTATATAAAAGAGAAACTACACGTAGCGCC6170.14491868582004716No Hit
GTCTTTAATCCTAACTGGAAAACTCCATCTTTTCCTGATATTCATTTGCA6080.14280479899285037No Hit
GTTAGTATTCCTTGGACTCACAAGGTGGGAAATTTTACTGGGCTTTACTC5900.13857702533845676No Hit
GTTAGAGGACCTACAAATTGTTCACATTTGTTAATAATGTCCTGGTGCAA5780.13575850956886104No Hit
GGATATCCACATTGTGTAAAAGGGGCAGCAAAACCCAAGAGACCCACAAT5750.13505388062646212No Hit
GGTGAGAGCTGAGGCGGTATCTAGAAGATCTCTAACTGAAGGAAAGAAGT5720.13434925168406317No Hit
CACAAAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGCTTAGATTG5700.1338794990557972No Hit
GGACATTATTAACAAATGTGAACAATTTGTAGGTCCTCTAACAGTAAATG5560.13059123065793551No Hit
CTTTTATATAAGATGCCCGCCTTCCATAGGGTATGTAAATAGTGTCTGGT5520.12965172540140363No Hit
GTATAAAACCTTATTATCCGGATAATGTGGTTAATCATTACTTCCAAACC5370.12612858068940896No Hit
GAATAAGGTGACCCACAAAATGAGGCGCTACGTGTAGTTTCTCTTTTATA5340.12542395174701002No Hit
TGTTAATAATGTCCTGGTGCAAATGAATATCAGGAAAAGATGGAGTTTTC5250.12331006491981324No Hit
GTTTAATCTTCGTTTTTCATTTACTGTTAGAGGACCTACAAATTGTTCAC5240.12307518860568026No Hit
GGGCCAAGTCTGTACAACATCTTGAGTCCCTTTATACCTCTGTTACCAAT5050.11861253863715367No Hit
CATTTGCACCAGGACATTATTAACAAATGTGAACAATTTGTAGGTCCTCT5010.11767303338062177No Hit
GCACCAGGACATTATTAACAAATGTGAACAATTTGTAGGTCCTCTAACAG5010.11767303338062177No Hit
TATCCACATTGTGTAAAAGGGGCAGCAAAACCCAAGAGACCCACAATTCT4990.11720328075235581No Hit
GTCTCTTCCTGCTGGTGGCTCCAGTTCCGGAACAGTGAACCCTGTTCCGA4960.11649865180995687No Hit
AGTCTGTACAACATCTTGAGTCCCTTTATACCTCTGTTACCAATTTTCTT4900.11508939392515902No Hit
GTATAGTAGAAGAGTAAAGCCCAGTAAAATTTCCCACCTTGTGAGTCCAA4770.1120360018414303No Hit
GATAATAAGGTTTTATACCTTTCTCTAGGGGCAAATATTTCGTAGAGATA4740.11133137289903138No Hit
CTTTTACACAATGTGGATATCCTGCTTTAATGCCTCTGTATGCGTGTATT4690.11015699132836648No Hit
CGCATGCGCTGATGGCCTATGGCCAAGCCCCATCCAGTGGGGGTTGCATC4540.1066338466163718No Hit
TAACAAATGTGAACAATTTGTAGGTCCTCTAACAGTAAATGAAAAACGAA4460.10475483610330799No Hit
CAAAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGCTTAGATTGAA4450.10451995978917501No Hit
GACTTCGCCCCCAATACCACATCATCCATATAACTGAAAGCCAGACAGTG4440.10428508347504203No Hit
ATGTAGCTCTTGTTCCCAAGAATAAGGTGACCCACAAAATGAGGCGCTAC4430.10405020716090907No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC4330.10170144401957928No Hit
GTATTTTCCTGCTGGTGGCTCCAGTTCCGGAACAGTGAACCCTGTTCCGA4330.10170144401957928No Hit
GCCTAGCAGCCATGGAAATGATGTATATTTCCGGGAGAGAACGACAGAAT4260.10005730982064845No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)