FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002488395

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488395
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences425756
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTAAACTTAGTCATGCCTGCTAGATTTTTTCCTATCTCTACGAAATAT31590.7419742763460762No Hit
CTACTATACCTGTCTTTAATCCTAACTGGAAAACTCCATCTTTTCCTGAT24370.5723935775420663No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN24090.565817040746343No Hit
TCCATAGGGTATGTAAATAGTGTCTGGTTTGGAAGTAATGATTAACCACA19720.46317609147023175No Hit
ATGTAAATAGTGTCTGGTTTGGAAGTAATGATTAACCACATTATCCGGAT16080.37768111312582797No Hit
ACTATACCTGTCTTTAATCCTAACTGGAAAACTCCATCTTTTCCTGATAT15850.3722789579007694No Hit
GTTTTATACCTTTCTCTAGGGGCAAATATTTCGTAGAGATAGGAAAAAAT13250.3112111162261953No Hit
AGTTCAACCTTCGTTTTTCATTTACTGTTAGAGGACCTACAAATTGTTCA12650.2971185373782166No Hit
AGTTTAATCTTCGTTTTTCATTTACTGTTAGAGGACCTACAAATTGTTCA12630.29664878474995066No Hit
GGTTGAACTTAGTCATGCCTGCTAGATTTTTTCCTATCTCTACGAAATAT12620.29641390843581766No Hit
TGTAAATAGTGTCTGGTTTGGAAGTAATGATTAACCACATTATCCGGATA11510.2703426375670572No Hit
TTCTTGGACTGTCCCTCTCGAATGGGGGAAGAATCATTCCACCACCAATC11350.2665846165409296No Hit
GTCCAAGAAAGCCCCATGATGTAGCTCTTGTTCCCAAGAATAAGGTGACC11170.26235684288653593No Hit
GTTCAACCTTCGTTTTTCATTTACTGTTAGAGGACCTACAAATTGTTCAC10590.24873401666682324No Hit
CTCTTCTACTATACCTGTCTTTAATCCTAACTGGAAAACTCCATCTTTTC10130.2379297062167063No Hit
GTATGTAAATAGTGTCTGGTTTGGAAGTAATGATTAACCACATTATCCGG9690.22759514839485526No Hit
CTTTAATCCTAACTGGAAAACTCCATCTTTTCCTGATATTCATTTGCACC9500.2231324984263287No Hit
CTTTATACCTCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAA9440.22172324054153086No Hit
CTTCTACTATACCTGTCTTTAATCCTAACTGGAAAACTCCATCTTTTCCT8280.19447758810210541No Hit
GTGTAAAAGGGGCAGCAAAACCCAAGAGACCCACAATTCTTTGACATACT8270.19424271178797245No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC8240.19353808284557353No Hit
CTGTTAGAGGACCTACAAATTGTTCACATTTGTTAATAATGTCCTGGTGC8060.18931030919117992No Hit
CAATCTCCAGCTTCCCAATGTTAGTATTCCTTGGACTCACAAGGTGGGAA7900.1855522881650523No Hit
GCTTTACTCTTCTACTATACCTGTCTTTAATCCTAACTGGAAAACTCCAT7750.18202914345305762No Hit
GGGTATGTAAATAGTGTCTGGTTTGGAAGTAATGATTAACCACATTATCC7730.18155939082479167No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCTCCCGTG7680.18038500925412676No Hit
CCTTTATACCTCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA7420.17427822508666935No Hit
TCCAAAGGGTATGTAAATAGTGTCTGGTTTGGAAGTAATGATTAACCACA7020.16488317252135026No Hit
CTATTTACATACCCTATGGAAGGCGGGCATCTTATATAAAAGAGAAACTA6920.16253440938002048No Hit
ACATTATCCGGATAATAAGGTTTTATACCTTTCTCTAGGGGCAAATATTT6900.16206465675175452No Hit
CCTACAAATTGTTCACATTTGTTAATAATGTCCTGGTGCAAATGAATATC6810.15995076992455773No Hit
ATTTGTAGGTCCTCTAACAGTAAATGAAAAACGAAGATTAAACTTAGTCA6770.15901126466802581No Hit
ACTGAGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCAGTTAG6590.15478349101363223No Hit
TTATTAACAAATGTGAACAATTTGTAGGTCCTCTAACAGTAAATGAAAAA6420.1507905936733716No Hit
GTCTTTAATCCTAACTGGAAAACTCCATCTTTTCCTGATATTCATTTGCA6270.14726744896137695No Hit
CTCTAACAGTAAATGAAAAACGAAGATTAAACTTAGTCATGCCTGCTAGA6240.146562820018978No Hit
GGATATCCACATTGTGTAAAAGGGGCAGCAAAACCCAAGAGACCCACAAT6130.14397918056351525No Hit
TTACATAACCCATGAAATTTAGGGAATATCCCCATCTTTTTGTTTTGTTG5990.14069091216565355No Hit
GTCTCTTCCTGCTGGTGGCTCCAGTTCCGGAACAGTGAACCCTGTTCCGA5920.13904677796672274No Hit
CCCTATGGAAGGCGGGCATCTTATATAAAAGAGAAACTACACGTAGCGCC5910.13881190165258975No Hit
GTTAGTATTCCTTGGACTCACAAGGTGGGAAATTTTACTGGGCTTTACTC5910.13881190165258975No Hit
GCACCAGGACATTATTAACAAATGTGAACAATTTGTAGGTCCTCTAACAG5860.13763752008192487No Hit
GTTAGAGGACCTACAAATTGTTCACATTTGTTAATAATGTCCTGGTGCAA5770.13552363325472805No Hit
TGTTAATAATGTCCTGGTGCAAATGAATATCAGGAAAAGATGGAGTTTTC5710.13411437536993018No Hit
TAATAAGGTTTTATACCTTTCTCTAGGGGCAAATATTTCGTAGAGATAGG5610.13176561222860042No Hit
CTTTTATATAAGATGCCCGCCTTCCATAGGGTATGTAAATAGTGTCTGGT5590.13129585960033446No Hit
GGGCCAAGTCTGTACAACATCTTGAGTCCCTTTATACCTCTGTTACCAAT5420.12730296226007384No Hit
GGACATTATTAACAAATGTGAACAATTTGTAGGTCCTCTAACAGTAAATG5330.12518907543287705No Hit
GTTTAATCTTCGTTTTTCATTTACTGTTAGAGGACCTACAAATTGTTCAC5290.12424957017634514No Hit
CATTTGCACCAGGACATTATTAACAAATGTGAACAATTTGTAGGTCCTCT5170.12143105440674941No Hit
GGTGAGAGCTGAGGCGGTATCTAGAAGATCTCTAACTGAAGGAAAGAAGT5110.12002179652195154No Hit
GTATAGTAGAAGAGTAAAGCCCAGTAAAATTTCCCACCTTGTGAGTCCAA5050.11861253863715367No Hit
GAATAAGGTGACCCACAAAATGAGGCGCTACGTGTAGTTTCTCTTTTATA5010.11767303338062177No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC4950.1162637754958239No Hit
CACAAAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGCTTAGATTG4920.11555914655342497No Hit
GTATAAAACCTTATTATCCGGATAATGTGGTTAATCATTACTTCCAAACC4900.11508939392515902No Hit
GTATTTTCCTGCTGGTGGCTCCAGTTCCGGAACAGTGAACCCTGTTCCGA4790.11250575446969625No Hit
TATCCACATTGTGTAAAAGGGGCAGCAAAACCCAAGAGACCCACAATTCT4720.11086162027076543No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGAGGGACGTCCTTTGTCTA4690.11015699132836648No Hit
CTTTAGGACATGGACATTGACCCTTATAAAGAATTTGGAGCTACTGAGGA4680.1099221150142335No Hit
GATAATAAGGTTTTATACCTTTCTCTAGGGGCAAATATTTCGTAGAGATA4660.10945236238596755No Hit
ATGTAGCTCTTGTTCCCAAGAATAAGGTGACCCACAAAATGAGGCGCTAC4660.10945236238596755No Hit
CCATAGGGTATGTAAATAGTGTCTGGTTTGGAAGTAATGATTAACCACAT4570.10733847555877075No Hit
CTTTTACACAATGTGGATATCCTGCTTTAATGCCTCTGTATGCGTGTATT4540.1066338466163718No Hit
ATTCTAATGTGTTCCTGTGGCAATGATCCCCAACTGCCAATTACATAACC4500.1056943413598399No Hit
TAACAAATGTGAACAATTTGTAGGTCCTCTAACAGTAAATGAAAAACGAA4490.10545946504570694No Hit
GTTTTCGAAAACAGTCTTTGATTTTCATTCTAATGTGTTCCTGTGGCAAT4420.10381533084677609No Hit
AGTCTGTACAACATCTTGAGTCCCTTTATACCTCTGTTACCAATTTTCTT4420.10381533084677609No Hit
GCTTAGATTGAATACACGCATACAGAGGCATTAAAGCAGGATATCCACAT4420.10381533084677609No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCTGCAATGTCAACGACCGACCT4390.10311070190437716No Hit
TCCTTGGACTCACAAGGTGGGAAATTTTACTGGGCTTTACTCTTCTACTA4360.10240607296197822No Hit
ACTGTGGAGTTACTCTCGTTTTTTGCCTTCTGACTTCTTTCCTTCAGTTA4300.10099681507718036No Hit
ATCTTCCTCTTCATCCTGCTGCTATGCCTCATCTTCTTGTTGGTTCTTCT4290.10076193876304737No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)