FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002488396

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488396
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences476750
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATATGTTCAGG36870.7733613004719454No Hit
CTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGATCATGCCTGCTAGG31030.6508652333508127No Hit
ATCCTAACCTTACCAAATATTTGCCCTTAGATAAAGGCATCAAACCTTAT27180.5701101206082853No Hit
GGATATTATCAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAACG25820.541583639223912No Hit
GTAGTAAGCTATGTCAATGTTAATATGGGCCTAAAAATCAGACAACTATT20130.42223387519664396No Hit
GGTTAGGATAGAACCTAGCAGGCATGATCAATTTTAATCTCCTTTTTTCG17320.3632931305715784No Hit
GGATAATAAGGTTTGATGCCTTTATCTAAGGGCAAATATTTGGTAAGGTT16810.3525957000524384No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTAGATAAAGGCATCAAAC14060.29491347666491874No Hit
GTTCAGGATAATAAGGTTTGATGCCTTTATCTAAGGGCAAATATTTGGTA13740.28820136339800734No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA13660.28652333508127953No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATA13290.2787624541164132No Hit
CTACTGTACCTGTCTTTAATCCTGAATGGCAAACTCCCTCTTTTCCTCAC13260.27813319349764026No Hit
CTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT13180.2764551651809124No Hit
CCCTTTACACAATGTGGATACCCAGCATTAATGCCTTTGTATGCATGTAT12740.2672260094389093No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA12690.26617724174095436No Hit
CTATATGTTCAGGATAATAAGGTTTGATGCCTTTATCTAAGGGCAAATAT12570.2636601992658626No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN12570.2636601992658626No Hit
GTGTAAAGGGAGCGGCAAAACCCAAAAGACCCACAATTCTTTGACATACT12210.25610907184058734No Hit
ATATGTTCCTGTGGCAAGGTACCCCAACTTCCAATTACATATCCCATGAA12120.2542212899842685No Hit
CATTTAAAAGAGGATATTATCAATAGATGTCAACAATATGTGGGCCCTCT11750.2464604090194022No Hit
TATCAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAACGAAAAAA11720.24583114840062925No Hit
ACTATATGTTCAGGATAATAAGGTTTGATGCCTTTATCTAAGGGCAAATA11640.24415312008390141No Hit
GAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATATGTTCA10940.22947037231253278No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC10830.22716308337703198No Hit
CTCTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT10720.2248557944415312No Hit
GGATAGAACCTAGCAGGCATGATCAATTTTAATCTCCTTTTTTCGTTAAC10710.2246460409019402No Hit
GTTTGGAAGTAATGATTAACTATATGTTCAGGATAATAAGGTTTGATGCC10180.21352910330361824No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTGATAATATCCTCTTTT10160.21310959622443626No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAGATC10020.21017304667016257No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG9970.20912427897220764No Hit
CAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAACGAAAAAAGGA9770.20492920818038804No Hit
ATTATCAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAACGAAAA9650.20241216570529627No Hit
CATTTAAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT9570.20073413738856843No Hit
CTCATAAGGTGGGAAACTTTACGGGACTTTATTCTTCTACTGTACCTGTC9390.19695857367593078No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCC9100.19087572102779235No Hit
ATTTATAGGAAGTTTTCTAAAACATTGTTTGATTTTTTGTACAATATGTT8590.18017829050865233No Hit
GTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACGGGACTTTATTC8100.16990036706869427No Hit
ATAATAAGGTTTGATGCCTTTATCTAAGGGCAAATATTTGGTAAGGTTAG7820.16402726796014683No Hit
CCTATAAATAGACCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGTCT7730.162139486103828No Hit
ATGTAAGACCTTGGGCAAGACCTGGCGGGCGTTCACGGTGGTCTCCATGC7730.162139486103828No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACCTTGCCACAG7710.16171997902464605No Hit
ATCTAAGGGCAAATATTTGGTAAGGTTAGGATAGAACCTAGCAGGCATGA7600.15941269008914524No Hit
GCTTACTACTGCTTCCCTGGATGCCGGGTCTTCCAAATTACTTCCCACCC7570.15878342947037233No Hit
GTATACATGCATACAAAGGCATTAATGCTGGGTATCCACATTGTGTAAAG7550.15836392239119035No Hit
ACTTTATTCTTCTACTGTACCTGTCTTTAATCCTGAATGGCAAACTCCCT7480.15689564761405347No Hit
ATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTTGGA7260.15228106974305192No Hit
ATTTACATACTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACT7200.15102254850550603No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACGGGACTTTATTCTTCTACTG7130.14955427372836916No Hit
CCTTTATCTAAGGGCAAATATTTGGTAAGGTTAGGATAGAACCTAGCAGG7070.14829575249082327No Hit
CTTTTAAATGAATGTGAGGAAAAGAGGGAGTTTGCCATTCAGGATTAAAG7060.14808599895123228No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA7000.14682747771368643No Hit
GATTAACTATATGTTCAGGATAATAAGGTTTGATGCCTTTATCTAAGGGC6850.1436811746198217No Hit
CTTTAATCCTGAATGGCAAACTCCCTCTTTTCCTCACATTCATTTAAAAG6770.14200314630309385No Hit
GTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCCTG6770.14200314630309385No Hit
GCCTAGCAGCCATGGAAAAGAGGTGTATTTCCGAGAGAGGACAACAGAGT6720.14095437860513896No Hit
GGTCTATTTATAGGAAGTTTTCTAAAACATTGTTTGATTTTTTGTACAAT6710.14074462506554797No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTTAAATGTATA6650.13948610382800208No Hit
CTTACAGTTAACGAAAAAAGGAGATTAAAATTGATCATGCCTGCTAGGTT6580.13801782905086524No Hit
GTAAGGAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATACTCAA6500.13633980073413737No Hit
ATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTTGGAAG6450.13529103303618248No Hit
ACAATATGTGGGCCCTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGA6400.13424226533822758No Hit
AGTCTGTACAACATCGTGAAGCCCTTTTTACCGCTATTACCAATTTTCTT6360.13340325117986365No Hit
TTATTATCCTGAACATATAGTTAATCATTACTTCCAAACTAGGCATTATT6220.13046670162558993No Hit
CAATATGTGGGCCCTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGAT6220.13046670162558993No Hit
ATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATATGTTCAGGAT6210.13025694808599897No Hit
GCCTTCCACAGAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAAC6180.129627687467226No Hit
GATTAAAATTGATCATGCCTGCTAGGTTCTATCCTAACCTTACCAAATAT6090.12773990561090717No Hit
GGAACATATTGTACAAAAAATCAAACAATGTTTTAGAAAACTTCCTATAA6040.12669113791295228No Hit
CTCAAGAACAGTTTCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACA6030.1264813843733613No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGAATGGCAAACTCCCTCTTTTC6020.1262716308337703No Hit
CTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGAAGAGAAACTGTTCT6000.12585212375458837No Hit
ATCCTGAACATATAGTTAATCATTACTTCCAAACTAGGCATTATTTACAT5970.1252228631358154No Hit
CTATTGATAATATCCTCTTTTAAATGAATGTGAGGAAAAGAGGGAGTTTG5890.12354483481908758No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCGTG5890.12354483481908758No Hit
CTTCCACAGAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTA5800.12165705296276876No Hit
ATAATATCCTCTTTTAAATGAATGTGAGGAAAAGAGGGAGTTTGCCATTC5730.12018877818563188No Hit
ATATGTGGGCCCTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGATCA5730.12018877818563188No Hit
GTACAACATCGTGAAGCCCTTTTTACCGCTATTACCAATTTTCTTTTGTC5680.11914001048767699No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC5640.11830099632931307No Hit
GCACTAAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATCTGGCCAC5590.11725222863135816No Hit
CTCCATATTCGGTGCAGGGTCCCCAGTCCTCGAGAAGATTGACGATATGG5580.11704247509176718No Hit
GTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC5560.1166229680125852No Hit
CCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGACA5550.11641321447299423No Hit
AAATAGACCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGTCTTTTGG5470.11473518615626639No Hit
GTGAATCCCTTTTTACCGCTATTACCAATTTTCTTTTGTCTTTGGGTATA5450.11431567907708443No Hit
GATAATATCCTCTTTTAAATGAATGTGAGGAAAAGAGGGAGTTTGCCATT5440.11410592553749344No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGCGGTAAAAA5430.11389617199790247No Hit
GTATAGGCCCCTCACTCTGGGATCTAGTAGAGCTTGGTGGAATGTGCTGG5400.11326691137912952No Hit
GATGTCAACAATATGTGGGCCCTCTTACAGTTAACGAAAAAAGGAGATTA5340.11200839014158363No Hit
CAACAATATGTGGGCCCTCTTACAGTTAACGAAAAAAGGAGATTAAAATT5280.11074986890403776No Hit
ATTGACAACAGTACCAGCAGCGCCTCCTCCTGCCTCCACCAATCGGCAGT5250.1101206082852648No Hit
GTTTCTCTCTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATG5230.10970110120608287No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGGCCGGCAGATGAGAAGGCACAG5220.10949134766649188No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAAGATGATGGGATGGGAATACAAG5210.10928159412690089No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGATTTCTTTCCTTCTATTCG4980.10445726271630833No Hit
CTTCTACTGTACCTGTCTTTAATCCTGAATGGCAAACTCCCTCTTTTCCT4980.10445726271630833No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCGTGAA4980.10445726271630833No Hit
CAATAGGTCTATTTATAGGAAGTTTTCTAAAACATTGTTTGATTTTTTGT4940.10361824855794441No Hit
ATTGATAATATCCTCTTTTAAATGAATGTGAGGAAAAGAGGGAGTTTGCC4940.10361824855794441No Hit
GTACAATATGTTCCTGTGGCAAGGTACCCCAACTTCCAATTACATATCCC4920.10319874147876244No Hit
ATCAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAACGAAAAAAG4860.10194022024121657No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGACCTGGCGGGCGTTC4860.10194022024121657No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT4840.1015207131620346No Hit
GTCCAAGGGATACTAACATTGAGATTCCCGAGATTGAGTTCTTCTGCGAC4810.10089145254326168No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)