FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002488397

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488397
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences476750
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATATGTTCAGG40670.853067645516518No Hit
CTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGATCATGCCTGCTAGG30280.6351337178814893No Hit
ATCCTAACCTTACCAAATATTTGCCCTTAGATAAAGGCATCAAACCTTAT25540.5357105401153645No Hit
GGATATTATCAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAACG24300.5097011012060828No Hit
GTAGTAAGCTATGTCAATGTTAATATGGGCCTAAAAATCAGACAACTATT19070.4No Hit
GGTTAGGATAGAACCTAGCAGGCATGATCAATTTTAATCTCCTTTTTTCG18770.39370739381227055No Hit
GGATAATAAGGTTTGATGCCTTTATCTAAGGGCAAATATTTGGTAAGGTT17400.3649711588883062No Hit
GTTCAGGATAATAAGGTTTGATGCCTTTATCTAAGGGCAAATATTTGGTA14970.31400104876769797No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA13600.2852648138437336No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTAGATAAAGGCATCAAAC13540.2840062926061877No Hit
CTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT13300.27897220765600417No Hit
CTATATGTTCAGGATAATAAGGTTTGATGCCTTTATCTAAGGGCAAATAT12830.2691137912952281No Hit
ATATGTTCCTGTGGCAAGGTACCCCAACTTCCAATTACATATCCCATGAA12630.26491872050340853No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN12540.2630309386470897No Hit
CTACTGTACCTGTCTTTAATCCTGAATGGCAAACTCCCTCTTTTCCTCAC12540.2630309386470897No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATA12400.26009438909281596No Hit
CCCTTTACACAATGTGGATACCCAGCATTAATGCCTTTGTATGCATGTAT12190.25568956476140536No Hit
GTGTAAAGGGAGCGGCAAAACCCAAAAGACCCACAATTCTTTGACATACT11870.24897745149449396No Hit
GAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATATGTTCA11850.248557944415312No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG11600.2433141059255375No Hit
GGATAGAACCTAGCAGGCATGATCAATTTTAATCTCCTTTTTTCGTTAAC11570.24268484530676457No Hit
ACTATATGTTCAGGATAATAAGGTTTGATGCCTTTATCTAAGGGCAAATA11200.23492396434189824No Hit
GTTTGGAAGTAATGATTAACTATATGTTCAGGATAATAAGGTTTGATGCC10990.23051914001048765No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC10800.22653382275825903No Hit
CTCTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT10780.22611431567907705No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTGATAATATCCTCTTTT10670.22380702674357633No Hit
TATCAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAACGAAAAAA10490.22003146303093862No Hit
CATTTAAAAGAGGATATTATCAATAGATGTCAACAATATGTGGGCCCTCT10260.21520713162034608No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA10190.21373885684320923No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAGATC10080.21143156790770842No Hit
CAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAACGAAAAAAGGA9800.20555846879916098No Hit
CATTTAAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT9300.19507079181961196No Hit
ATTTATAGGAAGTTTTCTAAAACATTGTTTGATTTTTTGTACAATATGTT9190.19276350288411115No Hit
ATTATCAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAACGAAAA8740.18332459360251704No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCC8420.17661248033560567No Hit
GCTTACTACTGCTTCCCTGGATGCCGGGTCTTCCAAATTACTTCCCACCC8260.17325642370214997No Hit
GTATACATGCATACAAAGGCATTAATGCTGGGTATCCACATTGTGTAAAG8210.17220765600419508No Hit
ATCTAAGGGCAAATATTTGGTAAGGTTAGGATAGAACCTAGCAGGCATGA7950.16675406397482956No Hit
CTCATAAGGTGGGAAACTTTACGGGACTTTATTCTTCTACTGTACCTGTC7850.16465652857891977No Hit
ATAATAAGGTTTGATGCCTTTATCTAAGGGCAAATATTTGGTAAGGTTAG7720.16192973256423704No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACCTTGCCACAG7610.15962244362873623No Hit
GTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCCTG7590.15920293654955428No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACGGGACTTTATTCTTCTACTG7500.15731515469323545No Hit
GTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACGGGACTTTATTC7450.15626638699528056No Hit
CCTATAAATAGACCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGTCT7290.15291033036182486No Hit
ATTTACATACTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACT7230.15165180912427897No Hit
ACTTTATTCTTCTACTGTACCTGTCTTTAATCCTGAATGGCAAACTCCCT7050.14787624541164132No Hit
CCTTTATCTAAGGGCAAATATTTGGTAAGGTTAGGATAGAACCTAGCAGG7020.14724698479286838No Hit
ATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTTGGA7010.1470372312532774No Hit
CTTTTAAATGAATGTGAGGAAAAGAGGGAGTTTGCCATTCAGGATTAAAG7000.14682747771368643No Hit
GGTCTATTTATAGGAAGTTTTCTAAAACATTGTTTGATTTTTTGTACAAT6870.14410068169900367No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA6860.14389092815941268No Hit
CTTTAATCCTGAATGGCAAACTCCCTCTTTTCCTCACATTCATTTAAAAG6780.14221289984268484No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTCTA6700.140534871525957No Hit
CAATATGTGGGCCCTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGAT6630.13906659674882013No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTTAAATGTATA6370.13361300471945464No Hit
GTAAGGAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATACTCAA6360.13340325117986365No Hit
AGTCTGTACAACATCGTGAAGCCCTTTTTACCGCTATTACCAATTTTCTT6300.13214472994231777No Hit
CTTACAGTTAACGAAAAAAGGAGATTAAAATTGATCATGCCTGCTAGGTT6240.13088620870477188No Hit
GATTAACTATATGTTCAGGATAATAAGGTTTGATGCCTTTATCTAAGGGC6230.1306764551651809No Hit
CTATTGATAATATCCTCTTTTAAATGAATGTGAGGAAAAGAGGGAGTTTG6210.13025694808599897No Hit
ATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATATGTTCAGGAT6210.13025694808599897No Hit
GGAACATATTGTACAAAAAATCAAACAATGTTTTAGAAAACTTCCTATAA6120.12836916622968012No Hit
CCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGACA6100.12794965915049816No Hit
GCCTAGCAGCCATGGAAAAGAGGTGTATTTCCGAGAGAGGACAACAGAGT6090.12773990561090717No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC6070.12732039853172522No Hit
GCCTTCCACAGAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAAC6070.12732039853172522No Hit
CTCAAGAACAGTTTCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACA6050.12690089145254327No Hit
CTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGAAGAGAAACTGTTCT6010.12606187729417934No Hit
ATATGTGGGCCCTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGATCA6000.12585212375458837No Hit
ATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTTGGAAG5980.1254326166754064No Hit
CTTCCACAGAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTA5950.12480335605663347No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGAATGGCAAACTCCCTCTTTTC5900.12375458835867856No Hit
TTATTATCCTGAACATATAGTTAATCATTACTTCCAAACTAGGCATTATT5880.12333508127949659No Hit
GTTTCTCTCTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATG5860.12291557420031463No Hit
ATCCTGAACATATAGTTAATCATTACTTCCAAACTAGGCATTATTTACAT5820.12207656004195071No Hit
ACAATATGTGGGCCCTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGA5720.1199790246460409No Hit
CTCCATATTCGGTGCAGGGTCCCCAGTCCTCGAGAAGATTGACGATATGG5690.11934976402726796No Hit
GTGAATCCCTTTTTACCGCTATTACCAATTTTCTTTTGTCTTTGGGTATA5610.11767173571054011No Hit
GATAATATCCTCTTTTAAATGAATGTGAGGAAAAGAGGGAGTTTGCCATT5550.11641321447299423No Hit
AAATAGACCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGTCTTTTGG5550.11641321447299423No Hit
GCACTAAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATCTGGCCAC5520.11578395385422129No Hit
GATTAAAATTGATCATGCCTGCTAGGTTCTATCCTAACCTTACCAAATAT5450.11431567907708443No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCGTG5450.11431567907708443No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGATTTCTTTCCTTCTATTCG5430.11389617199790247No Hit
GTACAACATCGTGAAGCCCTTTTTACCGCTATTACCAATTTTCTTTTGTC5340.11200839014158363No Hit
ATAATATCCTCTTTTAAATGAATGTGAGGAAAAGAGGGAGTTTGCCATTC5320.11158888306240168No Hit
GTCCAAGGGATACTAACATTGAGATTCCCGAGATTGAGTTCTTCTGCGAC5300.11116937598321971No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGCGGTAAAAA5270.11054011536444679No Hit
GTACAATATGTTCCTGTGGCAAGGTACCCCAACTTCCAATTACATATCCC5240.10991085474567383No Hit
GTATAGGCCCCTCACTCTGGGATCTAGTAGAGCTTGGTGGAATGTGCTGG5200.10907184058730991No Hit
CCTTTACCCCGTTGCTCGGCAACGGTCAGGTCTTTGCCAAGTGTTTGCTG5090.10676455165180912No Hit
GTTTGGGACTCTACCGTCCCCTTCTTCGTCTGCCGTTCCGACCGACCACG5090.10676455165180912No Hit
GATGTCAACAATATGTGGGCCCTCTTACAGTTAACGAAAAAAGGAGATTA5080.10655479811221813No Hit
CAACAATATGTGGGCCCTCTTACAGTTAACGAAAAAAGGAGATTAAAATT5080.10655479811221813No Hit
CAATAGGTCTATTTATAGGAAGTTTTCTAAAACATTGTTTGATTTTTTGT5030.10550603041426324No Hit
ATGTAAGACCTTGGGCAAGACCTGGCGGGCGTTCACGGTGGTCTCCATGC4970.10424750917671737No Hit
GTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC4950.1038280020975354No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGA4940.10361824855794441No Hit
ATTGACAACAGTACCAGCAGCGCCTCCTCCTGCCTCCACCAATCGGCAGT4890.10256948085998951No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAAGATGATGGGATGGGAATACAAG4830.10131095962244363No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGCCCGTGTG4820.10110120608285264No Hit
GCACGGGACCATGCAAGACCTGCACGACTCCTGCTCAAGGAACCTCTATG4780.10026219192448872No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG4780.10026219192448872No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCGTGAA4770.10005243838489773No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)