FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002488399

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488399
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences509834
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN76691.504215097463096No Hit
CATTTACAGGAGGACATTATTAATAGATGTCAACAATATGTGGGCCCTCT30000.588426821279083No Hit
GTATGTAAATAATGCCTGGTTTTGAAGTAATGATTAACTGCATGTTCAGG25680.503693359014895No Hit
GGATAATAAGGTTTAATGCCTTTCTCCAAGGGCAAATATTTGGTAAGGTT18640.36560919828807026No Hit
GAGTATGTAAATAATGCCTGGTTTTGAAGTAATGATTAACTGCATGTTCA17750.34815253592345746No Hit
CTTTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTGGT15240.29892082520977414No Hit
GGATAGTAAGGTTTAATGCCTTTCTCCAAGGGCAAATATTTGGTAAGGTT13680.2683226305032618No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC13500.2647920695755873No Hit
ATTTAAAGGAGGACATTATTAATAGATGTCAACAATATGTGGGCCCTCTT13230.25949622818407564No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGCTAATGAAAAA12120.2377244357967495No Hit
ATTTTAATCTCCTTTTTTCATTAGCTGTAAGAGGGCCCACATATTGTTGA11930.23399773259531534No Hit
CCCACAAAATGAAGCGCTGCGTGTAGTTTCTCTTTTATATAGAATGCCAG11330.22222919616973366No Hit
ATAATAAGGTTTAATGCCTTTCTCCAAGGGCAAATATTTGGTAAGGTTAG11220.22007163115837702No Hit
GACTCAAATTAATTATGCCTGCTAGGTTCTATCCTAACCTTACCAAATAT9940.19496542011713616No Hit
GTATACATGCATATAAAGGCTTCAAGGCAGGATAGCCACATTGTGTAAAA9620.18868886735682594No Hit
GTGTAAAAGGGGCAGCAAAGCCCAACAGACCCACAATTCTTTGACATACT9280.18202003004899633No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC9070.17790104230004275No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC9050.17750875775252337No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTGGAGAAAGGCATTAAAC9050.17750875775252337No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGAGAAAGGCATTAAACCTTAT9000.17652804638372488No Hit
TTTTTTGGGTCACCATATTCTTGGGAACAAGAGCTACAGCATGGGAGGTT8030.15750224582903455No Hit
GGGCCTCTACCGTCCCCTTCTTCATCTGCCGTTCCGGCCGACCACGGGGC7760.15220640443752279No Hit
GCTTAATTTTTAATACAATATGTTCCTGTGGTAAAGTACCCCAACTTCCA7730.1516179776162437No Hit
TCCACAGAGTATGTAAATAATGCCTGGTTTTGAAGTAATGATTAACTGCA7670.15044112397368556No Hit
CATTTAAAGGAGGACATTATTAATAGATGTCAACAATATGTGGGCCCTCT7630.14965655487864676No Hit
GGATTAAAGACAGGTACAGTAGAAGAATAAAGCCCAGTAAAGTTTCCCAC7610.14926427033112738No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGAGAAAGGCATTAAACCTTAC7610.14926427033112738No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA7560.1482835589623289No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG7550.14808741668856923No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA7450.14612599395097228No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTGTACCTGTC7430.14573370940345287No Hit
TTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTGGTTT7430.14573370940345287No Hit
CTTCAATCCTGAGTGGCAAACTCCCTCCTTTCCTAACATTCATTTAAAGG7350.14416457121337534No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAACATTCATTTACAGG7340.14396842893961564No Hit
GCCAAACAGTGGGGGAAAGCCCTGCGAACCACTGAACAAATGGCACTAGT7260.14239929074953808No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA7060.13847644527434422No Hit
GTCCACAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGAAGAGGA6950.13631888026298755No Hit
CTTTTACACAATGTGGCTATCCTGCCTTGAAGCCTTTATATGCATGTATA6810.13357288843035184No Hit
CCTTTAAATGAATGTTAGGAAAGGAGGGAGTTTGCCACTCAGGATTAAAG6810.13357288843035184No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC6790.13318060388283245No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCTGGTCTCTGCCAAGTGTTTGCTG6790.13318060388283245No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTCAGGCA6720.13180760796651458No Hit
CTTTAGGACATGGACATCGACCCGTATAAAGAATTTGGAGCTTCTGCCGA6600.12945390068139828No Hit
CTTCTGGACTACCAGGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGAAC6580.12906161613387887No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG6530.1280809047650804No Hit
ATATTAACGTTGACATAGCTGACTACTAATTCCCTGGATGCTGGGTCTTC6470.12690405112252223No Hit
GGGTAAAGGTGCAGGTATTGTTGACGCAGAAAGGCCTTGTAAGTTGGCGA6470.12690405112252223No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCCGCTTACAGACCACCAAATGCC6400.1255310552062044No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA6370.12494262838492529No Hit
GTAGACGCCCAAACGGCCCCGAGACGGGTCGTCCGCGGGATTCAGCGCCG6310.12376577474236712No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG6160.1208236406359717No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT6100.11964678699341355No Hit
CGTTAATATGGGCCTAAAAATCAGACAGCTATTGTGGTTTCACATTTCCT6090.11945064471965385No Hit
CCTTTAAATGAATGTTAGGAAAGGAGGGAGTTTGCCACTCAGGATTGAAG6090.11945064471965385No Hit
GATTAAAATTAATTATGCCTGCTAGGTTCTATCCTAACCTTACCAAATAT5960.11690079516077781No Hit
TTTAAAGGAGGACATTATTAATAGATGTCAACAATATGTGGGCCCTCTTA5760.11297794968558393No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGAAACACCCGCG5750.11278180741182424No Hit
CCCCTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTG5720.11219338059054516No Hit
TTATTATCCTGAACATGCAGTTAATCATTACTTCAAAACCAGGCATTATT5700.11180109604302577No Hit
ATTTACAGGAGGACATTATTAATAGATGTCAACAATATGTGGGCCCTCTT5690.11160495376926609No Hit
ATAGTAAGGTTTAATGCCTTTCTCCAAGGGCAAATATTTGGTAAGGTTAG5620.11023195785294822No Hit
CTTCAATCCTGAGTGGCAAACTCCCTCCTTTCCTAACATTCATTTACAGG5580.10944738875790944No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA5570.10925124648414974No Hit
CTCTTACAGCTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG5530.10846667738911096No Hit
AAATCAAGCAATGTTTTCGGAAACTGCCTGTAAATCGACCTATTGATTGG5510.1080743928415916No Hit
CCCAAAAAATGAAGCGCTGCGTGTAGTTTCTCTTTTATATAGAATGCCAG5380.10552454328271554No Hit
AGTTAAGGGAGTAGCCCCAGCGTTTGGTTTTATTAGGGTTCAAATGTATA5340.10473997418767678No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTGAGTCTCCTTTTTTCA5290.1037592628188783No Hit
CTCCATGTTCGGTGCAGGGTCCCCAGTCCTCGAGAAGATTGACGATATGG5290.1037592628188783No Hit
CCTAATCTCCTCCCCCAACTCCTCCCAGTCCTTAAACAAACAGTCTTTGA5290.1037592628188783No Hit
CTGTATGGCCTGAGGATGACTGTCTCTTAGAGGTGGAGAGATGGGAGTAG5210.10219012462880075No Hit
GGATTGAAGACAGGTACAGTAGAAGAATAAAGCCCAGTAAAGTTTCCCAC5200.10199398235504105No Hit
CCTTACTATCCTGAACATGCAGTTAATCATTACTTCAAAACCAGGCATTA5200.10199398235504105No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAACATTCATTTAAAGG5180.10160169780752165No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAAAGAGAAACTACA5180.10160169780752165No Hit
TCCCATGGCTGCTCGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCC5170.10140555553376197No Hit
TTACTATCCTGAACATGCAGTTAATCATTACTTCAAAACCAGGCATTATT5100.1000325596174441No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)