FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488400

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488400
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences529075
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN49680.9389973066200443No Hit
TCCATAGAGTGTGTAAATAATGTCTGGTTAGGAAGTAATGATTAACTCGA38100.7201247460189953No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACT18930.3577942635732174No Hit
GGATAAGGGTATTAAACCTTATTATCCAGAACATCGAGTTAATCATTACT17160.32433964938808296No Hit
GTGTAGTTTCTCTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAA14280.26990502291735574No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT14260.26952700467797575No Hit
GTATTAAACCTTATTATCCAGAACATCGAGTTAATCATTACTTCCTAACC14180.26801493172045554No Hit
CATTTAAACCCTAACAAAACAAAAAGGTGGGGTTACTCTTTACATTTTAT13070.24703491943486272No Hit
CTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTA12990.24552284647734254No Hit
GTTTTATCCAAAGGTTACAAAATATTTGCCATTGGATAAGGGTATTAAAC12470.23569437225346124No Hit
GTTTAATACCCTTATCCAATGGCAAATATTTTGTAACCTTTGGATAAAAC12350.23342626281718093No Hit
GTGTAAATAATGTCTGGTTAGGAAGTAATGATTAACTCGATGTTCTGGAT12020.2271889618674101No Hit
AACTCGATGTTCTGGATAATAAGGTTTAATACCCTTATCCAATGGCAAAT12020.2271889618674101No Hit
ATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTAGGA11200.21169021405282804No Hit
CCCTTATCCAATGGCAAATATTTTGTAACCTTTGGATAAAACCTAGCAGG11000.20791003165902758No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC9890.18693001937343479No Hit
ATCCAAAGGTTACAAAATATTTGCCATTGGATAAGGGTATTAAACCTTAT9770.18466190993715448No Hit
CTTTTCACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAA9350.17672352691017343No Hit
GGATAATAAGGTTTAATACCCTTATCCAATGGCAAATATTTTGTAACCTT9250.17483343571327317No Hit
CACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACTCACAGT9250.17483343571327317No Hit
CTCTTCTACTGTTCCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTC8920.16859613476350233No Hit
AATATACATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGG8920.16859613476350233No Hit
CTTCTACTGTTCCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTCCT8470.1600907243774512No Hit
GTGTAAAAGGGGCAGCAAACCCCAAAAGACCCACAATACGTTGACAGACT8370.15820063318055097No Hit
CCAAATACTCTATGACCGTTTCTCTTCCAAAAGTAAGACAAGAAATGTGA8280.15649955110334074No Hit
CTCACAGTCAATGAGAAAAGAAGACTGCAATTGATCATGCCTGCTAGGTT8180.15460945990644048No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG7990.15101828663233002No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACATTCTTTGAGTTTCTGT7950.15026225015356992No Hit
GGTTACAAAATATTTGCCATTGGATAAGGGTATTAAACCTTATTATCCAG7880.14893918631573974No Hit
TAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTAGGAAGT7800.14742711335821954No Hit
GAAAAGAAGACTGCAATTGATCATGCCTGCTAGGTTTTATCCAAAGGTTA7660.14478098568255918No Hit
GTTCTGGATAATAAGGTTTAATACCCTTATCCAATGGCAAATATTTTGTA7650.14459197656286918No Hit
GTGTGTAAATAATGTCTGGTTAGGAAGTAATGATTAACTCGATGTTCTGG7400.13986674857061854No Hit
GTCAATGAGAAAAGAAGACTGCAATTGATCATGCCTGCTAGGTTTTATCC7400.13986674857061854No Hit
GGATAACCACATTGTGTAAAAGGGGCAGCAAACCCCAAAAGACCCACAAT7330.13854368473278836No Hit
CTACAAACCTTGCCAACAAATCCGCCTCCTGCCTCTACCAATCGCCAGTC7200.13608656617681802No Hit
CCTCAAGCTTATAGACCAGCAAATGCCCCTATCTTATCAACACTTCCGGA7020.1326844020223976No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC7010.13249539290270756No Hit
ATGTGTAGTTTCTCTCTTATATAATATACCCGCCTTCCATAGAGTGTGTA7000.1323063837830175No Hit
TTATTATCCAGAACATCGAGTTAATCATTACTTCCTAACCAGACATTATT6960.13155034730425744No Hit
ATATTTGCCATTGGATAAGGGTATTAAACCTTATTATCCAGAACATCGAG6960.13155034730425744No Hit
CTTTAACCCTCATTGGAAAACACCCTCTTTTCCTAATATACATTTACACC6770.12795917403014695No Hit
CATCATACAGAAAATCAAAGAATGTTTTAGAAAACTTCCTGTTAACAGGC6220.11756367244719558No Hit
ATGTAAAGAGTAACCCCACCTTTTTGTTTTGTTAGGGTTTAAATGTATAC6200.11718565420781553No Hit
CACCCTCTTTTCCTAATATACATTTACACCAAGACATTATCAAAAAATGT6120.11567358125029532No Hit
GATCATGCCTGCTAGGTTTTATCCAAAGGTTACAAAATATTTGCCATTGG6100.11529556301091527No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCTTTGTATGCATGTATT6090.11510655389122525No Hit
GGTTTAATACCCTTATCCAATGGCAAATATTTTGTAACCTTTGGATAAAA6020.11378349005339507No Hit
TTCTTTGGGTTACCATATTCTTGGGAACAAGAGCTACAGCATGGGGCAGA5930.11208240797618485No Hit
GTTAGTATTCCTTGGACTCATAAGGTGGGAAACTTTACGGGGCTTTACTC5890.11132637149742475No Hit
TTGATTTTCTGTATGATGTGATCTTGTGGCAATGACCCATAACTTCCAAT5890.11132637149742475No Hit
GGGTATTAAACCTTATTATCCAGAACATCGAGTTAATCATTACTTCCTAA5890.11132637149742475No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA5890.11132637149742475No Hit
CTCTTTACATTTTATGGGCTATGTCATTGGAAGTTATGGGTCATTGCCAC5810.10981429853990454No Hit
AGTCTGTACAGCACCTTGAGTCCCTTTTCACCGCTGTTACCAATTTTCTT5790.10943628030052449No Hit
GTAATGATTAACTCGATGTTCTGGATAATAAGGTTTAATACCCTTATCCA5770.10905826206114445No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTC5720.10811321646269433No Hit
GTATTAAACCTTATTATCCAGAACATCGAGTTAATCATTACTTCCTAACCAGACATTATTTACACACTCTATGGA5630.1064121343854841No Hit
TCCATAGAGTGTGTAAATAATGTCTGGTTAGGAAGTAATGATTAACTCGATGTTCTGGATAATAAGGTTTAATAC5540.10471105230827388No Hit
GGTTTGTAGTATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGGCCTCCGT5500.10395501582951379No Hit
ATCTAGAAGATCTCGTACGGAAGGAAAGAAGTCAGAAGGCAAAAACGAGA5470.1033879884704437No Hit
CTCATAAGGTGGGAAACTTTACGGGGCTTTACTCTTCTACTGTTCCTGTC5440.10282096111137362No Hit
GTGTTGTTTCTCTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAA5420.10244294287199357No Hit
ATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTCACGGTGGTCTCCATGC5350.1011198790341634No Hit
CTTTATACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAA5340.10093086991447336No Hit
CTAATATACATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTA5300.10017483343571328No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)