FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488401

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488401
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences529075
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN49660.9386192883806644No Hit
TCCATAGAGTGTGTAAATAATGTCTGGTTAGGAAGTAATGATTAACTCGA37350.7059490620422435No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACT18360.347020743750886No Hit
GGATAAGGGTATTAAACCTTATTATCCAGAACATCGAGTTAATCATTACT17420.32925388650002363No Hit
GTATTAAACCTTATTATCCAGAACATCGAGTTAATCATTACTTCCTAACC15110.28559277985162784No Hit
GTTTAATACCCTTATCCAATGGCAAATATTTTGTAACCTTTGGATAAAAC14300.2702830411567358No Hit
GTGTAGTTTCTCTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAA13990.264423758446345No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT13550.25610735717998395No Hit
CATTTAAACCCTAACAAAACAAAAAGGTGGGGTTACTCTTTACATTTTAT13450.2542172659830837No Hit
GTTTTATCCAAAGGTTACAAAATATTTGCCATTGGATAAGGGTATTAAAC12500.2362613996125313No Hit
CTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTA12290.23229220809904078No Hit
AACTCGATGTTCTGGATAATAAGGTTTAATACCCTTATCCAATGGCAAAT12100.2287010348249303No Hit
GTGTAAATAATGTCTGGTTAGGAAGTAATGATTAACTCGATGTTCTGGAT11520.21773850588290883No Hit
CCCTTATCCAATGGCAAATATTTTGTAACCTTTGGATAAAACCTAGCAGG11120.21017814109530786No Hit
GGTTACAAAATATTTGCCATTGGATAAGGGTATTAAACCTTATTATCCAG10660.2014837215895667No Hit
GGATAATAAGGTTTAATACCCTTATCCAATGGCAAATATTTTGTAACCTT10620.2007276851108066No Hit
ATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTAGGA9870.18655200113405473No Hit
ATCCAAAGGTTACAAAATATTTGCCATTGGATAAGGGTATTAAACCTTAT9570.18088172754335397No Hit
CTTTTCACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAA9390.1774795633889335No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC8920.16859613476350233No Hit
GTGTAAAAGGGGCAGCAAACCCCAAAAGACCCACAATACGTTGACAGACT8810.16651703444691207No Hit
CACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACTCACAGT8770.16576099796815197No Hit
CCAAATACTCTATGACCGTTTCTCTTCCAAAAGTAAGACAAGAAATGTGA8760.16557198884846194No Hit
CTCTTCTACTGTTCCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTC8650.16349288853187166No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG8480.16027973349714122No Hit
CCTCAAGCTTATAGACCAGCAAATGCCCCTATCTTATCAACACTTCCGGA8190.1547984690261305No Hit
AATATACATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGG8160.15423144166706043No Hit
GTTCTGGATAATAAGGTTTAATACCCTTATCCAATGGCAAATATTTTGTA8030.1517743231110901No Hit
CTTCTACTGTTCCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTCCT8000.15120729575202005No Hit
ATATTTGCCATTGGATAAGGGTATTAAACCTTATTATCCAGAACATCGAG7430.14043377592968861No Hit
GTGTGTAAATAATGTCTGGTTAGGAAGTAATGATTAACTCGATGTTCTGG7380.1394887303312385No Hit
CTCACAGTCAATGAGAAAAGAAGACTGCAATTGATCATGCCTGCTAGGTT7330.13854368473278836No Hit
TAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTAGGAAGT7320.13835467561309833No Hit
GGATAACCACATTGTGTAAAAGGGGCAGCAAACCCCAAAAGACCCACAAT7320.13835467561309833No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACATTCTTTGAGTTTCTGT7310.1381656664934083No Hit
CTTTAACCCTCATTGGAAAACACCCTCTTTTCCTAATATACATTTACACC7240.13684260265557813No Hit
GAAAAGAAGACTGCAATTGATCATGCCTGCTAGGTTTTATCCAAAGGTTA7230.13665359353588813No Hit
TTATTATCCAGAACATCGAGTTAATCATTACTTCCTAACCAGACATTATT7130.13476350233898787No Hit
ATGTGTAGTTTCTCTCTTATATAATATACCCGCCTTCCATAGAGTGTGTA7070.1336294476208477No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC6990.1321173746633275No Hit
CTACAAACCTTGCCAACAAATCCGCCTCCTGCCTCTACCAATCGCCAGTC6860.12966025610735718No Hit
CATCATACAGAAAATCAAAGAATGTTTTAGAAAACTTCCTGTTAACAGGC6670.12606908283324672No Hit
GGTTTAATACCCTTATCCAATGGCAAATATTTTGTAACCTTTGGATAAAA6580.12436800075603648No Hit
GTCAATGAGAAAAGAAGACTGCAATTGATCATGCCTGCTAGGTTTTATCC6310.1192647545244058No Hit
GTCTTTGGGTATACATTTAAACCCTAACAAAACAAAAAGGTGGGGTTACT6210.11737466332750554No Hit
GGGTATTAAACCTTATTATCCAGAACATCGAGTTAATCATTACTTCCTAA5900.11151538061711476No Hit
ATGTAAAGAGTAACCCCACCTTTTTGTTTTGTTAGGGTTTAAATGTATAC5870.11094835325804471No Hit
GTATCACCCTGCTGGTGGCTCCAGTTCAGGAACAGTAAACCCTGTTCCGA5820.11000330765959458No Hit
GATCATGCCTGCTAGGTTTTATCCAAAGGTTACAAAATATTTGCCATTGG5800.10962528942021453No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCTTTGTATGCATGTATT5770.10905826206114445No Hit
AGTCTGTACAGCACCTTGAGTCCCTTTTCACCGCTGTTACCAATTTTCTT5750.1086802438217644No Hit
GGTTTGTAGTATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGGCCTCCGT5670.1071681708642442No Hit
CTCTATGACCGTTTCTCTTCCAAAAGTAAGACAAGAAATGTGAAACCACA5630.1064121343854841No Hit
CACCCTCTTTTCCTAATATACATTTACACCAAGACATTATCAAAAAATGT5620.10622312526579407No Hit
CTCTTTACATTTTATGGGCTATGTCATTGGAAGTTATGGGTCATTGCCAC5600.10584510702641402No Hit
CTCATAAGGTGGGAAACTTTACGGGGCTTTACTCTTCTACTGTTCCTGTC5570.10527807966734395No Hit
GTATTAAACCTTATTATCCAGAACATCGAGTTAATCATTACTTCCTAACCAGACATTATTTACACACTCTATGGA5540.10471105230827388No Hit
GTTAGTATTCCTTGGACTCATAAGGTGGGAAACTTTACGGGGCTTTACTC5490.10376600670982375No Hit
TCCATAGAGTGTGTAAATAATGTCTGGTTAGGAAGTAATGATTAACTCGATGTTCTGGATAATAAGGTTTAATAC5420.10244294287199357No Hit
TTCTTTGGGTTACCATATTCTTGGGAACAAGAGCTACAGCATGGGGCAGA5380.10168690639323348No Hit
CTTTATACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAA5320.10055285167509331No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)