FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488402

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488402
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences514466
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN45530.8849953155310555No Hit
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA38520.7487375259006426No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT14390.27970750253661075No Hit
GGATAAGGGTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACT13810.2684336768610559No Hit
CTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTT12880.250356680519218No Hit
GTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAAC11810.2295584159108668No Hit
GTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT10650.2070107645597571No Hit
GTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACC10440.20292886215998723No Hit
CAATAGGCCTATTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTCTGT10260.19943008867447024No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC10120.19670882040795698No Hit
CATTTAAACCCTAACAAAACAAGAAGGTGGGGTTACTCTTTACATTTTAT9750.18951689713217199No Hit
GGATAATAAGGTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATT9580.1862124999514059No Hit
ATCCAAATGTTACCAAATATTTGCCATTGGATAAGGGTATTAAACCTTAT9260.17999245819937565No Hit
CCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAACCTAGCAGG9220.17921495298037188No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACT9190.17863182406611905No Hit
ATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGA9120.17727118993286242No Hit
GAAGACTGCAATTGATTATGCCTGCTAGGTTTTATCCAAATGTTACCAAA9040.17571617949485485No Hit
GGGACCTAGTAGTCAGTTATGTTAACACTAATATGGGCCTAAAGTTCAGG8930.17357804014259445No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACGTAGCGCC8310.16152670924803583No Hit
GTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC8150.1584166883720207No Hit
GTACCGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC8000.15550104380075652No Hit
TAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGT7670.1490866257439753No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTCTCTAGGGGGAACTACCGTG7640.14850349682972247No Hit
CTACAAACCTTGCCAACAAATCCGCCTCCTGCCTCTACCAATCGCCAGTC7470.1451990996489564No Hit
CTCTAAGACCGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA7220.14033969203018276No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT6630.12887149004987697No Hit
GATTATGCCTGCTAGGTTTTATCCAAATGTTACCAAATATTTGCCATTGG6300.12245707199309576No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC6150.11954142742183158No Hit
GGTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAA5990.11643140654581643No Hit
GTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGG5920.11507077241255982No Hit
GCTTTAGGGCATGGACATTGATCCTTATAAAGAATTTGGAGCTTCTGTGG5900.11468201980305794No Hit
TTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACCAGACATTATT5840.11351576197455227No Hit
CACTAATATGGGCCTAAAGTTCAGGCAACTATTGTGGTTTCACATTTCTT5720.11118324631754091No Hit
GTTCTGGATAATAAGGTTTAATACCCTTATCCAATGGCAAATATTTGGTA5710.11098887001278995No Hit
GTCTTAGAGTATTTAGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCACC5670.11021136479378618No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAAAGCCTTTGTATGCCTGTATT5670.11021136479378618No Hit
CGAGCAGATGAGGCATAGCAGCAGGATGAAGAGGAATATGATAAAACGCC5510.10710134391777104No Hit
CTCATAAGGTGGGAAACTTTACGGGGCTTTACTCTTCTTCTGTTCCTGTC5390.1047688282607597No Hit
ATCTAGAAGATCTCGTATGGAAGGAAAGAAGTCATGAGGCAAAAACGAGA5310.10321381782275214No Hit
GTTTTATCCAAATGTTACCAAATATTTGCCATTGGATAAGGGTATTAAAC5280.1026306889084993No Hit
ATGCTGTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAG5250.10204755999424647No Hit
CATTTACACCAAGACATTATCGAAAAATGTGAACAATTTGTAGGCCCACT5250.10204755999424647No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)