FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488403

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488403
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences514466
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN45350.8814965420455385No Hit
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA38500.7483487732911407No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT14410.28009625514611264No Hit
GTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAAC13010.2528835724809803No Hit
GGATAAGGGTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACT12060.23441782352964044No Hit
CTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTT12040.23402907092013855No Hit
GTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT11970.23266843678688195No Hit
GTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACC10450.2031232384647382No Hit
CATTTAAACCCTAACAAAACAAGAAGGTGGGGTTACTCTTTACATTTTAT10080.1959313151889532No Hit
CCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAACCTAGCAGG10060.19554256257945132No Hit
ATCCAAATGTTACCAAATATTTGCCATTGGATAAGGGTATTAAACCTTAT9550.1856293710371531No Hit
GGATAATAAGGTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATT9300.18076996341837945No Hit
GTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC9110.17707681362811148No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC9000.17493867427585108No Hit
CAATAGGCCTATTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTCTGT8990.1747442979711001No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTCTCTAGGGGGAACTACCGTG8890.17280053492359068No Hit
ATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGA8830.171634277095085No Hit
GAAGACTGCAATTGATTATGCCTGCTAGGTTTTATCCAAATGTTACCAAA8760.17027364296182837No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACGTAGCGCC8200.15938856989577543No Hit
GTACCGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC8180.15899981728627355No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACT8110.1576391831530169No Hit
CTCTAAGACCGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA7570.14714286269646584No Hit
GGGACCTAGTAGTCAGTTATGTTAACACTAATATGGGCCTAAAGTTCAGG7470.1451990996489564No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT7280.14150594985868842No Hit
TAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGT7050.13703529484941668No Hit
CTACAAACCTTGCCAACAAATCCGCCTCCTGCCTCTACCAATCGCCAGTC6720.13062087679263548No Hit
GGTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAA6600.12828836113562414No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC6580.12789960852612223No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAAAGCCTTTGTATGCCTGTATT6520.12673335069761654No Hit
GTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGG6280.12206831938359386No Hit
GCTTTAGGGCATGGACATTGATCCTTATAAAGAATTTGGAGCTTCTGTGG6150.11954142742183158No Hit
GTCTTAGAGTATTTAGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCACC6020.11701453546006928No Hit
TTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACCAGACATTATT5910.11487639610780888No Hit
GATTATGCCTGCTAGGTTTTATCCAAATGTTACCAAATATTTGCCATTGG5890.11448764349830699No Hit
GTTCTGGATAATAAGGTTTAATACCCTTATCCAATGGCAAATATTTGGTA5890.11448764349830699No Hit
CTCATAAGGTGGGAAACTTTACGGGGCTTTACTCTTCTTCTGTTCCTGTC5620.10923948327003145No Hit
CACTAATATGGGCCTAAAGTTCAGGCAACTATTGTGGTTTCACATTTCTT5520.10729572022252198No Hit
GTTTTATCCAAATGTTACCAAATATTTGCCATTGGATAAGGGTATTAAAC5480.10651821500351823No Hit
CTATAAGGTGGAGGAGTGCGAATCCACACTCCGAAAGACACTAAATACTC5380.10457445195600876No Hit
CTCTTTACATTTTATGGGCTATGTCATTGGATGTTATGGGTCATTGCCAC5260.10224193629899742No Hit
CCTCCACCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA5200.10107567847049174No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)