FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488404

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488404
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences457369
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN25420.5557875588419854No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG24930.5450741086518763No Hit
CTTATATAGAATGCCCGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT22600.49413055978870457No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGACAAAGGCATTAAACCATAT20880.4565241632030155No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA15470.33823892743058664No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCGCTTACAGTTAATGAAAAA12870.2813920488708242No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT11990.2621515668967508No Hit
CCTCCTGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA11950.2612769995342929No Hit
CTCTTATATAGAATGCCCGCCTTCCACAGAGTATGTAAATAATGCCTAGT11900.2601837903312205No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC10050.21973504981754338No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA9910.2166740640489408No Hit
ACCAAGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGAACATCAACTACC9700.2120825853960369No Hit
ATGTAATTGGATGTTGGGGTACTTTACCGCAAGAACATATTGTACTAAAA9620.21033345067112114No Hit
TGTCTGTTCCGATCAGTTTCGCTCCAGACCGGCTGCGAGCAAAACAAGCT9600.2098961669898922No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC9510.20792839042436195No Hit
CTGACATACTTTCCAATCAATAGGTCTATTTACAGGGAGTTTTCGAAAAC8920.19502852182810815No Hit
GAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCA8820.19284210342196345No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC8710.19043704317520427No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTATTACATAACCT8680.18978111765336086No Hit
GAACCGACAACTCTGTTGTCCTCTCTCGGAAATACACCTCCTTTCCATGG8500.18584556452230036No Hit
GTCTATTTACAGGGAGTTTTCGAAAACATTGCTTGATTTTTAGTACAATA8280.18103544402878202No Hit
GGATAATATGGTTTAATGCCTTTGTCCAAGGGCAAATATTTGGTAAGGTT8170.17863038378202284No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTGGACAAAGGCATTAAAC8140.17797445826017944No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG8080.17666260721649257No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC8050.17600668169464917No Hit
ATCCTAACCTAACCAAATATTTGCCCTTGGACAAAGGCATTAAACCATAT8000.17491347249157682No Hit
GGGTATACATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTC7730.1690101427949861No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA7660.1674796499106848No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTACTGTGGTTTCACATTTCC7590.16594915702638352No Hit
GGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCATTAAC7510.16420002230146774No Hit
GTATACATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTCAT7480.16354409677962434No Hit
CTATTAATAATGTCCTCCTGTAAATGAATGTGAGGAAAGGAGGGAGTTTG7460.16310681309839536No Hit
ATATTATCCTGAACATGCAGTTAATCATTACTTCAAAACTAGGCATTATT7270.15895261812672043No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG7250.1585153344454915No Hit
CAATAGGTCTATTTACAGGGAGTTTTCGAAAACATTGCTTGATTTTTAGT7060.15436113947381655No Hit
CATTTACAGGAGGACATTATTAATAGATGTCAACAATATGTGGGCCCGCT7060.15436113947381655No Hit
GTGTAAAAGGGGCAGCAAAGCCCAACAGACCCACAATCCTCTGACATACT7050.15414249763320206No Hit
ATACCTTCCTGCTGGTGGCTCCAGTTCCGGAACAGTAAACCCTGTTCCGA7040.1539238557925876No Hit
ATTTACAGGGAGTTTTCGAAAACATTGCTTGATTTTTAGTACAATATGTT6990.15283064658951523No Hit
ATTGACAACAGTGCCAGTAGCACCTCCTCCTGCCTCCACCAATCGGCAGT6940.15173743738644288No Hit
CTTATATAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTTTCCA6840.14955101898029818No Hit
GCAGATGAAGAAGGGGACGGTAGAGTCCCAAACGGCCCCGAGACGGGTCG6650.14539682400862322No Hit
GGAAAGTATAGGCCCCTCACTCTGGGGTCTAGCAGAGCTTGGTGGAATGT6590.14408497296493641No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTCATTTCAT6490.14189855455879172No Hit
GGTCTATTTACAGGGAGTTTTCGAAAACATTGCTTGATTTTTAGTACAAT6410.14014941983387594No Hit
CCTATTACATAACCTAATCTCCTCCCCCAACTCCTCCCAGTCCTTAAACA6400.13993077799326145No Hit
CTTACATAAGAGGACTCTTGGACTCGCAGCAATGTCAACGACCGACCTTG6380.1394934943120325No Hit
ATATGTAATTGGATGTTGGGGTACTTTACCGCAAGAACATATTGTACTAA6380.1394934943120325No Hit
GTCTAACAACAGTAGTTTCCGGAAGTGTTGATAAGATAGGGGCATTTGGT6340.13861892694957464No Hit
GTATACATGCATATAAAGGCTTTAAGGCAGGATAGCCACATTGTGTAAAA6270.13708843406527335No Hit
CCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT6260.13686979222465886No Hit
GTTTGTAGACGACGAGGCAGGTCCCCTAGAAGAAGAACTCCCTCGCCTCG6250.13665115038404438No Hit
GGATTAAAGACAGGTACAGTAGAAGAATAAAGCCCAGTAAAGTTTCCCAC6250.13665115038404438No Hit
CGCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG6240.13643250854342992No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAG6230.13621386670281543No Hit
GTTGTTAGACGACGAGGCAGGTCCCCTAGAAGAAGAACTCCCTCGCCTCG6030.131841029890526No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCA5970.1305291788468392No Hit
GGGAATTAGTAGTCAGCTATGTCAATGTTAATATGGGCCTAAAAATCAGA5870.1283427604406945No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTCAC5760.1259377001939353No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTCACATTCATTTACAGG5750.12571905835332084No Hit
ATTACATATCCCATGAAATGAAGGGAGTAGCCCCAACGTTTGGTTTTATT5740.12550041651270635No Hit
GTCTTACATAAGAGGACTCTTGGACTCGCAGCAATGTCAACGACCGACCT5710.12484449099086296No Hit
CTTTTTACCTCTATTACCAATTTTCTTTTGTCTCTGGGTATACATTTGAA5590.12222078890348931No Hit
CCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGACA5400.11806659393181435No Hit
AATGTATACCCAGAGACAAAAGAAAATTGGTAATAGAGGTAAAAAGGGAC5260.11500560816321174No Hit
ACCTTGAGGCATACTTCAAAGACTGTTTGTTTAAGGACTGGGAGGAGTTG5250.1147869663225973No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGCGAGTCCAA5240.11456832448198283No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTGTACCTGTC5200.11369375711952492No Hit
GTTTTGAAGTAATGATTAACTGCATGTTCAGGATAATATGGTTTAATGCC5150.11260054791645258No Hit
CTATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATA5130.11216326423522363No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC5080.11107005503215128No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTTTCCATGC5050.11041412951030788No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC5040.1101954876696934No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTG4930.10779042742293421No Hit
ACCTAATCTCCTCCCCCAACTCCTCCCAGTCCTTAAACAAACAGTCTTTG4860.10625993453863292No Hit
GTTCAGGATAATATGGTTTAATGCCTTTGTCCAAGGGCAAATATTTGGTA4850.10604129269801844No Hit
TGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTG4840.10582265085740399No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA4830.10560400901678951No Hit
ATATGGTTTAATGCCTTTGTCCAAGGGCAAATATTTGGTAAGGTTAGGAT4830.10560400901678951No Hit
ATCCTGAACATGCAGTTAATCATTACTTCAAAACTAGGCATTATTTACAT4820.10538536717617504No Hit
ATTTCATATCCCATGAAATGAAGGGAGTAGCCCCAACGTTTGGTTTTATT4820.10538536717617504No Hit
CAATTACATATCCCATGAAATGAAGGGAGTAGCCCCAACGTTTGGTTTTA4780.10451079981371714No Hit
TGTATACCCAGAGACAAAAGAAAATTGGTAATAGAGGTAAAAAGGGACTC4750.10385487429187375No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTCAGGCA4730.10341759061064479No Hit
CCTTCTGACTTCTTTCCTTCTATTCGAGATCTCCTCGACACCGCCTCAGC4680.10232438140757244No Hit
ACCAGGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGAACATCAACTACC4660.1018870977263435No Hit
CCTCTGACATACTTTCCAATCAATAGGTCTATTTACAGGGAGTTTTCGAA4610.10079388852327115No Hit
ATCCTCTGACATACTTTCCAATCAATAGGTCTATTTACAGGGAGTTTTCG4590.1003566048420422No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)